1990 Fiscal Year Annual Research Report

食品成分による生体防御の機作

Research Project

Project/Area Number	02218102
Research Institution	The University of Tokyo
Principal Investigator	多田富雄東京大学, 医学部(医), 教授 (10009136)
Co-Investigator(Kenkyū-buntansha)	栃倉辰六郎京都大学, 農学部, 教授 (70026524) 鈴木紘一東京都臨床医学総合研究所, 遺伝情報研究部, 部長 (80011948) 高久史磨東京大学, 医学部(病), 教授 (40048955) 上野川修一東京大学, 農学部, 教授 (50011945) 白井俊一順天堂大学, 医学部, 教授 (30115860)
Keywords	生体防御 / 免疫 / 消化管免疫 / 抗体 / リンパ球
Research Abstract	多田班員は、消化管がBおよびT細胞の分化の場として重要であること、ことに胸腺にしか存在しないはずの未分化のCD4^+CD8^+T細胞が消化管に存在することを証明した。白井班員は、自己免疫動物の発症過程で、低脂肪低カロリ-食が自己抗体産生を抑制し、発病率を低下させることを証明した。これはアレルギ-にも応用可能であった。上野川班員は、食品抗原ds_<1ー>カゼイン分子内で、TおよびB細胞を刺激し得るエピト-プの分布を解明した。それぞれのエピト-プの立体的位置関係が、食品アレルギ-成立に関与していることがわかった。また免疫抑制因子を産生するT細胞クロ-ンを得た。村上班員は、食品中に含まれるインタ-フェロン誘導物質を追跡し、比較的単純な多糖類等に顕著な活性を認めた。それらが消化管を通して吸収され、生体防御に関与していることを示した。高久班員は、食品アレルギ-で問題になる好酸球の誘導に働く造血因子としてILー5とGMーCSFの共同作業が存在することを証明した。鈴木班員は、食肉の熟成に関与しているCa^<2+>結合性プロテインキナ-ゼCの基本構造を決定した。さらにカルパインが細胞活性化において転写因子制御していることを証明した。栃倉班員は、ビフィズス菌生着因子、ビフィズス菌由来の酵素の生体防御におよぼす影響を調べた。吉田班員は、解毒に関与するチトクロ-ムP450の生成課程でのRNA転写の生体内条件、ことにアスコルビン酸について解析した。

Research Products

(30 results)

All Other

All Publications (30 results)

[Publications] 長澤敏行: "免疫細胞分化の場としての消化管" 細胞工学. 19. 795-801 (1990)
[Publications] Horiuchi,T.: "Epitope specific regulation of the antibody response against αーlactoalbumins in the mouse." Int.Arch.Allergy Appl.Immunol.80. (1990)
[Publications] Nagasawa,T.: "Thymus dependent CD4^+8^+ intraepithelial lymphocytes induced in response to the bacterial colonization."
[Publications] Shirai,T.: "The expanted population of CD4^ー CD8^ー α/β^+ T cells associated with the <lpr>___ー and <old>___ー mutations are CD2^ー." J.Immunol.144. 3756-3761 (1990)
[Publications] Okada,T.: "Distinct surface pehnotypes of B cells responsible for spontaneous production of IgM and IgG antiーDNA antibodies in autoimmuneーprone NZB×NZW F_1 mice." Autoimmunity. 7. 109-120 (1990)
[Publications] Hasegawa,K.: "Monoclonal antibodies to epitope of CD45R (B220) inhibit interleikin 4ーmediated B cell prolireration and differentiation." Int.Immunol.2. 367-375 (1990)
[Publications] Ogawa,S.: "Nucleotide sequence analysis of MHC class II gene autoimmuneーprone(NZB×NZW)F_1 mice." Immunogenetics. 32. 63-67 (1990)
[Publications] Hirose,S.: "Effects of major histocompatibility complex on autoimmune disease of Hー2ーcongenic mice." Int.Immunol.2. 1091-1095 (1990)
[Publications] Shibata,T.: "Monoclonal antiーerythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms." Int.Immunol.2. 1133-1131 (1990)
[Publications] Enomoto,A.: "Antibodies raised against peptide fragments of bovine αslーcasein crossーreact with the intact protein only when the peptides contain both B and T cell determinants." Molec.Immunol.27. 581-586 (1990)
[Publications] Hachimura,S.: "Relative positioning of the T cell and B cell determinants on an immunogenic peptide:its influence on antibody response." Biochem.Biophs.Res.Commun.169. 803-808 (1990)
[Publications] Hisatsune,T.: "CD8^+ suppressor T cell clone capable of inhibiting the antigenーand antiーT cell receptorーinduced proliferation of Th clones without cytolytic activity." J.Immunol.145. 2421-2426 (1990)
[Publications] Yamada,K.: "Stimulation of proliferation and immunoglobulin M production by lactoferrin in humanーhuman and mouseーmouse hybridomas in serum free culture." Cytotechnology. 3. 123-131 (1990)
[Publications] Toyota,K.: "Murification and characterization of immunoglobulin production stimulating factor derived from human B lymphoblastoid HOー323 cells." Cytotechnology. 3. 189-197 (1990)
[Publications] Yamada,K.: "Effect of immunoglobulin production stimulating factors in fondstufts in immunoglobulin production of human lymphobytes." Agric.Biol.Chem.54. 1087-1089 (1990)
[Publications] Yamada,K.: "Proliferationーstimulating activity of detergents having oleic acid residue in serumーfree culture of mammalian cells." Agric.Biol.Chem.54. 1867-1868 (1990)
[Publications] Yamada,K.: "Stimulation and inhibition of interferon β production of human diploid fibroblasts by food stuffs." Agric.Biol.Chem.55. 829-832 (1991)
[Publications] AKita,Y.: "Expression and properties of two distinct classes of the phordoll ester receptor family,four conventional protein kinase C types,and a novel protein Kinase C." J.Biol.Chem.265. 354-362 (1990)
[Publications] Ohno,S.: "A point mutation of the putative ATPーbinding site of protein Kinase C alpha abolishes the Kinase activity and renders it downーregulation insensitive." J.Biol.Chem.265. 6296-6300 (1990)
[Publications] Suzuki,K.: "Calciumーactivated neutral proteaseーstructureーfunction relationship and functional implications." Cell Structure and Function. 15. 1-6 (1990)
[Publications] Ohno,S.: "Four genes for the calpain family located on four distinct human chromosomes." Cytogenet.Cell Genet.53. 225-229 (1990)
[Publications] Akita,Y.: "Possible role of Ca^<2+>ーindependent protein kinase C epsilon,in thyrotropin releasing hormone stimulated signal transduction." Biochem.Biophys.Res.Commun.172. 184-189 (1990)
[Publications] Sorimachi,H.: "A novel member of calcilumーdependent cysteinr protease family." Biol.Chem.HoppeーSeyler. 371. 171-176 (1990)
[Publications] Oda,H.: "Hyperlipoproteinemia in rats fed polychlorinated biphentyls." J.Nutr.Sci.Vitaninol.36. 117-122 (1990)
[Publications] Nagaoka,S.: "Effects of dietary polychlorinated biphenyls on cholesterol catabolism in rats." Brit.J.Nutrition. 64. 161-169 (1990)
[Publications] Yoshida,A.: "Comparative studies on soy protein and rice protein for tholesterol metabolism in rats." J.Nutr.Sci.Vitaminol.36. 137-139 (1990)
[Publications] Nagaoka,S.: "Effects of excess dietary tyrosine or cholesterol,bile acid metabolism and mixed function oxidase system in rats." J.Nutrition. 130. 1134-1139 (1990)
[Publications] 吉田昭: "生体異物による代謝変動と栄養(総説)" 日本農芸化学会誌. 64. 1343-1348 (1990)
[Publications] 堀尾文彦: "ビタミンCと薬物代謝(ミニレビュ-)" 日本農芸化学会誌. 64. 1854-1857 (1990)
[Publications] 矢野俊博: "ビヒズス菌の選択増殖と関連酸素機構" 酪農科学・食品の研究. 40. (1991)

1990 Fiscal Year Annual Research Report

食品成分による生体防御の機作

Principal Investigator

多田 富雄 東京大学, 医学部(医), 教授 (10009136)

Research Products

[Publications] 長澤 敏行: "免疫細胞分化の場としての消化管" 細胞工学. 19. 795-801 (1990)

[Publications] Horiuchi,T.: "Epitope specific regulation of the antibody response against αーlactoalbumins in the mouse." Int.Arch.Allergy Appl.Immunol.80. (1990)

[Publications] Nagasawa,T.: "Thymus dependent CD4^+8^+ intraepithelial lymphocytes induced in response to the bacterial colonization."

[Publications] Shirai,T.: "The expanted population of CD4^ー CD8^ー α/β^+ T cells associated with the <lpr>___ー and <old>___ー mutations are CD2^ー." J.Immunol.144. 3756-3761 (1990)

[Publications] Okada,T.: "Distinct surface pehnotypes of B cells responsible for spontaneous production of IgM and IgG antiーDNA antibodies in autoimmuneーprone NZB×NZW F_1 mice." Autoimmunity. 7. 109-120 (1990)

[Publications] Hasegawa,K.: "Monoclonal antibodies to epitope of CD45R (B220) inhibit interleikin 4ーmediated B cell prolireration and differentiation." Int.Immunol.2. 367-375 (1990)

[Publications] Ogawa,S.: "Nucleotide sequence analysis of MHC class II gene autoimmuneーprone(NZB×NZW)F_1 mice." Immunogenetics. 32. 63-67 (1990)

[Publications] Hirose,S.: "Effects of major histocompatibility complex on autoimmune disease of Hー2ーcongenic mice." Int.Immunol.2. 1091-1095 (1990)

[Publications] Shibata,T.: "Monoclonal antiーerythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms." Int.Immunol.2. 1133-1131 (1990)

[Publications] Enomoto,A.: "Antibodies raised against peptide fragments of bovine αslーcasein crossーreact with the intact protein only when the peptides contain both B and T cell determinants." Molec.Immunol.27. 581-586 (1990)

[Publications] Hachimura,S.: "Relative positioning of the T cell and B cell determinants on an immunogenic peptide:its influence on antibody response." Biochem.Biophs.Res.Commun.169. 803-808 (1990)

[Publications] Hisatsune,T.: "CD8^+ suppressor T cell clone capable of inhibiting the antigenーand antiーT cell receptorーinduced proliferation of Th clones without cytolytic activity." J.Immunol.145. 2421-2426 (1990)

[Publications] Yamada,K.: "Stimulation of proliferation and immunoglobulin M production by lactoferrin in humanーhuman and mouseーmouse hybridomas in serum free culture." Cytotechnology. 3. 123-131 (1990)

[Publications] Toyota,K.: "Murification and characterization of immunoglobulin production stimulating factor derived from human B lymphoblastoid HOー323 cells." Cytotechnology. 3. 189-197 (1990)

[Publications] Yamada,K.: "Effect of immunoglobulin production stimulating factors in fondstufts in immunoglobulin production of human lymphobytes." Agric.Biol.Chem.54. 1087-1089 (1990)

[Publications] Yamada,K.: "Proliferationーstimulating activity of detergents having oleic acid residue in serumーfree culture of mammalian cells." Agric.Biol.Chem.54. 1867-1868 (1990)

[Publications] Yamada,K.: "Stimulation and inhibition of interferon β production of human diploid fibroblasts by food stuffs." Agric.Biol.Chem.55. 829-832 (1991)

[Publications] AKita,Y.: "Expression and properties of two distinct classes of the phordoll ester receptor family,four conventional protein kinase C types,and a novel protein Kinase C." J.Biol.Chem.265. 354-362 (1990)

[Publications] Ohno,S.: "A point mutation of the putative ATPーbinding site of protein Kinase C alpha abolishes the Kinase activity and renders it downーregulation insensitive." J.Biol.Chem.265. 6296-6300 (1990)

[Publications] Suzuki,K.: "Calciumーactivated neutral proteaseーstructureーfunction relationship and functional implications." Cell Structure and Function. 15. 1-6 (1990)

[Publications] Ohno,S.: "Four genes for the calpain family located on four distinct human chromosomes." Cytogenet.Cell Genet.53. 225-229 (1990)

[Publications] Akita,Y.: "Possible role of Ca^<2+>ーindependent protein kinase C epsilon,in thyrotropin releasing hormone stimulated signal transduction." Biochem.Biophys.Res.Commun.172. 184-189 (1990)

[Publications] Sorimachi,H.: "A novel member of calcilumーdependent cysteinr protease family." Biol.Chem.HoppeーSeyler. 371. 171-176 (1990)

[Publications] Oda,H.: "Hyperlipoproteinemia in rats fed polychlorinated biphentyls." J.Nutr.Sci.Vitaninol.36. 117-122 (1990)

[Publications] Nagaoka,S.: "Effects of dietary polychlorinated biphenyls on cholesterol catabolism in rats." Brit.J.Nutrition. 64. 161-169 (1990)

[Publications] Yoshida,A.: "Comparative studies on soy protein and rice protein for tholesterol metabolism in rats." J.Nutr.Sci.Vitaminol.36. 137-139 (1990)

[Publications] Nagaoka,S.: "Effects of excess dietary tyrosine or cholesterol,bile acid metabolism and mixed function oxidase system in rats." J.Nutrition. 130. 1134-1139 (1990)

[Publications] 吉田 昭: "生体異物による代謝変動と栄養(総説)" 日本農芸化学会誌. 64. 1343-1348 (1990)

[Publications] 堀尾 文彦: "ビタミンCと薬物代謝(ミニレビュ-)" 日本農芸化学会誌. 64. 1854-1857 (1990)

[Publications] 矢野 俊博: "ビヒズス菌の選択増殖と関連酸素機構" 酪農科学・食品の研究. 40. (1991)

多田富雄東京大学, 医学部(医), 教授 (10009136)

[Publications] 長澤敏行: "免疫細胞分化の場としての消化管" 細胞工学. 19. 795-801 (1990)

[Publications] Shirai,T.: "The expanted population of CD4^ー CD8^ー α/β^+ T cells associated with the <lpr>_ー and <old>_ー mutations are CD2^ー." J.Immunol.144. 3756-3761 (1990)

[Publications] 吉田昭: "生体異物による代謝変動と栄養(総説)" 日本農芸化学会誌. 64. 1343-1348 (1990)

[Publications] 堀尾文彦: "ビタミンCと薬物代謝(ミニレビュ-)" 日本農芸化学会誌. 64. 1854-1857 (1990)

[Publications] 矢野俊博: "ビヒズス菌の選択増殖と関連酸素機構" 酪農科学・食品の研究. 40. (1991)