1990 Fiscal Year Annual Research Report

シンク機能発現と物質変換

Research Project

Project/Area Number	02261101
Research Institution	Toyama Medical and Pharmaceutical University
Principal Investigator	西荒介富山医科薬科大学, 薬学部, 教授 (50019098)
Co-Investigator(Kenkyū-buntansha)	吉原照彦北海道大学, 農学部, 助教授 (90002071) 南川隆雄東京都立大学, 理学部, 教授 (30087001) 橋本隆京都大学, 農学部, 助手 (80180826) 茅野充男東京大学, 農学部, 教授 (10007677) 大山莞爾京都大学, 農学部, 教授 (40135546)
Keywords	物質集積 / シンク器官 / 貯臓物質 / 二次代謝 / 遺伝情報
Research Abstract	本研究は2つのグル-プの協力によって進められた。第1のグル-プは植物のシンク機能に関係する遺伝子の構造と発現調節を調べ、第2のグル-プは同機能の発現に影響する生理活性物質の解明を目的としている。第1グル-プの大山は光合成細菌の形質転換系を用い、ゼニゴケ葉緑体の未知遺伝子と相同性を示す遺伝子のクロ-ニングを行った。たん白や酵素遺伝子の発現調節機構を検討するため、南川はマメの種子の数種のたん白や酵素について、新名は西洋ワサビのペルオキシダ-ゼについて、それぞれの遺伝子の構造を解析すると共に、そのプロモ-タ-領域をレポ-タ-遺伝子につないで異種植物に導入し発現の様子を調べた。小林はシロイヌナズナの組織やカルスを用い、光合成遺伝子の転写活性とDNAのメチル化の関係を調べた。。庄野は細菌型インド-ル酢酸合成経路の少くとも後半の部分は植物にも存在することを確め、その生理機能の検討を進めている。第2のグル-プで吉原はキクイモからツベロン酸外数種の塊茎形成物質を単離、またタマネギの鱗茎形成物質を追求している。一方、山根は鱗茎形成抑制物質と考えられるジベレリンの作用を検討するため、その分布と変動を調べた。橋本はヒヨシアミン水酸化酵素の抗体を用い、細胞免疫化学的に同酵素の分布を調べ、根の内鞘での特異的発現を認めた。茅野はダイズ貯臓たん白の遺伝子を得て、それを異種植物に導入し、栄養による発現調節がダイズと同様におこることを確めた。西はニンジンのファイトアレキシンの生合成経路を明らかにし、またエリシタ-の刺激伝達に関係するとみられる各種の要因を調べた。上野もエンバクのファイトアレキシンに関し、その化学合成に成功すると共に組織内分布を調べ、抵抗性との関係を検討している。坂神はエンドウで種子のみに存在するオ-キシン、4ーCLーIAAの量的変化を種子の成熟段階を追って測定し、その集積機能における役割を調べた。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Kurosaki,F.: "Comparison of enzyme activities involved in the biosynthesis of carrot phytoalexin 6ーmethoxymellein" Phytochemistry.
[Publications] Ueno,T.: "Seconday metabolites related to host selection by plant pathogenic fungi" Pure & Appl.Chem.62. 1347-1352 (1990)
[Publications] Hamada,N.: "Lecanolic acid from the mycobiont of the lichen Stereocaulon curtatum" Phytochemistry. 29. 678-679 (1990)
[Publications] Kitajima,Y.: "Revised structure of the peptide lactone antibiotic,TLー119/or Aー3302ーB" FEBS Letters. 270. 139-142 (1990)
[Publications] Ogura,Y.: "Gene encoding a putative zinc finger protein in Synechocystis PCC6803"
[Publications] Ngernprasirtsiri,J.: "Application of an efficient strategy with a phage λ vector for constructing a physical map of the amyloplast genome of sycamore(Acer pseudoplatanus)" Arch.Biochem.Biophys.276. 172-179 (1990)
[Publications] Ishibashi,M.: "Molecular cloning and characterization of stored mRNA in cotyledons of Vigna unguiculata seeds" Plant & Cell Physiol.31. 39-44 (1990)
[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells" FEBS Lett.260. 127-130 (1990)
[Publications] Ishibashi,M.: "Stored mRNA in cotyledons of Vigna unguiculata seed:nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination" Plant Mol.Biol.15. 59-64 (1990)
[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase(SHーEP)from Vigna mungo" Nucleic Acids Res.18. 1892 (1990)
[Publications] Yamauchi,D.: "Nucleotide sequence of cDNA for αーamylase from cotyledone of germinating Vigna mungo" Nucleic Acids Res.18. 4250 (1990)
[Publications] Haraguchi,H.: "Multiple forms of acid phosphatase in cotyledons of Vigna mungo seedling:immunological detection and quantitation" Plant & Cell Physiol.31. 917-923 (1990)
[Publications] Kawaguchi,M.: "Isolation of Rhizobium leguminosarum biovar viciae variants with indoleー3ーacetamide hydrolase activity" Plant & Cell Physiol.31. 449-455 (1990)
[Publications] Kawaguchi,M.: "The presence of an enzyme that converts indolー3ーacetーamide into IAA in wild and cultivated rice" Plant & Cell Physiol.
[Publications] Fujiyama,K.: "Genomic DNA structure of two horseradish peroxidase genes" Gene. 89. 163-169 (1990)
[Publications] Intapruk,C.: "Nucleotide sequences of two genomic DNAs encoding peroxidase of Arabidopsis thalinana" Gene.
[Publications] Fujiwara,T.: "Electroported protoplasts express seed specific gene promotor" Plant Cell Reports.
[Publications] Hashimoto,T.: "Hyoscyamine 6βーhydroxylase,an enzyme involved in tropane alkaloid biosynthesis,is localized at the pericycle of the root" J.Biol.Chem.

1990 Fiscal Year Annual Research Report

シンク機能発現と物質変換

Principal Investigator

西 荒介 富山医科薬科大学, 薬学部, 教授 (50019098)

Research Products

[Publications] Kurosaki,F.: "Comparison of enzyme activities involved in the biosynthesis of carrot phytoalexin 6ーmethoxymellein" Phytochemistry.

[Publications] Ueno,T.: "Seconday metabolites related to host selection by plant pathogenic fungi" Pure & Appl.Chem.62. 1347-1352 (1990)

[Publications] Hamada,N.: "Lecanolic acid from the mycobiont of the lichen Stereocaulon curtatum" Phytochemistry. 29. 678-679 (1990)

[Publications] Kitajima,Y.: "Revised structure of the peptide lactone antibiotic,TLー119/or Aー3302ーB" FEBS Letters. 270. 139-142 (1990)

[Publications] Ogura,Y.: "Gene encoding a putative zinc finger protein in Synechocystis PCC6803"

[Publications] Ngernprasirtsiri,J.: "Application of an efficient strategy with a phage λ vector for constructing a physical map of the amyloplast genome of sycamore(Acer pseudoplatanus)" Arch.Biochem.Biophys.276. 172-179 (1990)

[Publications] Ishibashi,M.: "Molecular cloning and characterization of stored mRNA in cotyledons of Vigna unguiculata seeds" Plant & Cell Physiol.31. 39-44 (1990)

[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells" FEBS Lett.260. 127-130 (1990)

[Publications] Ishibashi,M.: "Stored mRNA in cotyledons of Vigna unguiculata seed:nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination" Plant Mol.Biol.15. 59-64 (1990)

[Publications] Akasofu,H.: "Nucleotide sequence of the gene for sulfhydrylーendoーpeptidase(SHーEP)from Vigna mungo" Nucleic Acids Res.18. 1892 (1990)

[Publications] Yamauchi,D.: "Nucleotide sequence of cDNA for αーamylase from cotyledone of germinating Vigna mungo" Nucleic Acids Res.18. 4250 (1990)

[Publications] Haraguchi,H.: "Multiple forms of acid phosphatase in cotyledons of Vigna mungo seedling:immunological detection and quantitation" Plant & Cell Physiol.31. 917-923 (1990)

[Publications] Kawaguchi,M.: "Isolation of Rhizobium leguminosarum biovar viciae variants with indoleー3ーacetamide hydrolase activity" Plant & Cell Physiol.31. 449-455 (1990)

[Publications] Kawaguchi,M.: "The presence of an enzyme that converts indolー3ーacetーamide into IAA in wild and cultivated rice" Plant & Cell Physiol.

[Publications] Fujiyama,K.: "Genomic DNA structure of two horseradish peroxidase genes" Gene. 89. 163-169 (1990)

[Publications] Intapruk,C.: "Nucleotide sequences of two genomic DNAs encoding peroxidase of Arabidopsis thalinana" Gene.

[Publications] Fujiwara,T.: "Electroported protoplasts express seed specific gene promotor" Plant Cell Reports.

[Publications] Hashimoto,T.: "Hyoscyamine 6βーhydroxylase,an enzyme involved in tropane alkaloid biosynthesis,is localized at the pericycle of the root" J.Biol.Chem.

西荒介富山医科薬科大学, 薬学部, 教授 (50019098)