1991 Fiscal Year Annual Research Report

昆虫後期発生におけるタンパク質生合成ならびに機能発現調節機構

Research Project

Project/Area Number	02304009
Research Institution	Tokyo Metropolitan University
Principal Investigator	富野士良東京都立大学, 理学部, 教授 (30101075)
Co-Investigator(Kenkyū-buntansha)	芦田正明北海道大学, 低温科学研究所, 教授 (50012422) 名取俊二東京大学, 薬学部, 教授 (50012662) 大西英爾岡山理科大学, 理学部, 教授 (60022521) 高橋進山口大学, 教養部, 教授 (90022665) 山下興亜名古屋大学, 農学部, 教授 (50023411)
Keywords	ビテロゲニン / 卵特異的タンパク質 / cDNA / 遺伝子クロ-ニング / フェノ-ルオキシダ-ゼ / アリルホリン / 脂質動員ホルモン / 脂肪体
Research Abstract	本総合研究Aの平成3年度の研究成果の概要は以下のとおりである。 1.イエバエのビテロゲニンmRNAレベルの変動をRNAブロツテイング法にて解析した。雌では脂肪体中のビテロゲニンmRNA量の変動は体液中ビテロゲニンと平行関係にあった。雌雄の遊離腹部を用いた実験から、脱皮ホルモンがビテロゲニン合成を誘導することが判明した。カイコ・ビテロゲニン・サブユニットのNー末端の一次構造とcDNAの塩基配列の比較から、H鎖とし鎖は一本のmRNAより翻訳生成されることを確認した。カイコ卵特異的タンパク質の全塩基配列を決定,推定アミノ酸配列は哺乳類リパ-ゼと高い相同性を示した。カイコSP1受容体タンパク質と脂肪体細胞膜上に同定した。 2.カイコ・フェノ-ルオキシダ-ゼの活性化に関与するタンパク質を分離精製し,治性化カスケ-ドを再構築後,反応機構の解析を行った。カイコ・外皮ラッカ-ゼおよびショウジョウバエ・プロフェノ-ルオキシダ-ゼのアイソザイムを分離精製した。ショウジョウバエではプロパノ-ルによってもフェノ-ルオキシダ-ゼが活性化されることを確認。 3.ニクバエ・アリルホリン遺伝子5´上流域の塩基配列ACCACAACAに特異的に結合するタンパク質を幼虫脂肪体より精製した。このタンパク質は40Kと42Kの2種のサブユニットより構成され、ニクバエ胚由来の培養細胞核より調製した無細胞転写系でアリルホリン遺伝子の正確な転写を促進した。 4.トノサマバッタにおける脂質動員ホルモン(AKH)と長距離飛翔との関連性について解析した。AKHの分泌により血中トレハロ-スが減少し、トレハロ-スによるジグリセリドの積み込みが解除され,脂肪体から飛翔筋への大量の脂質輸送が開始されることが判明した。

Research Products

(22 results)

All Other

All Publications (22 results)

[Publications] Mori,S.: "Structure and organization of genes for major plasma proteins of Bombyx mori." J.Mol.Biol.218. 7-12 (1991)
[Publications] Mori,S.: "Complete nucleotide sequence of major plasma protein genes of Bombyx mori." Biochim.Biophys.Acta. 1090. 129-132 (1991)
[Publications] Agui,N: "Hormonal control of vitellogenin mRNA level in the male andfemale housefly,Musca domestica." J.Insect Physiol.37. 383-390 (1991)
[Publications] Itoh,M.: "Cloning and sequence analysis of membrane bound alkaline phosphaase cDNA of the silkworm,Bombyx mori." Biochim.Biophys.Acta. 1129. 135-138 (1991)
[Publications] Yaginuma.T.: "Does an enzyme activity capable of phosphorylating sorbitol control utilization of sorbitol at the termination of diapause in eggs of the silkworm Bombyx mori." Comp.Biochem.Biophys.98B. 135-141 (1991)
[Publications] Sato,Y.: "Synthesis and secretion of eggーspecific protein from follicle cells of the silkworm,Bombyx mori." Insect Biochem.21. 233-238 (1991)
[Publications] Ikeda,M.: "cDNA cloning,sequencing and temporal expression of the protease responsible for vitellin degradation in the silkworm,Bombyx mori." Comp.Biochem.Pysiol.99B. 405-411 (1991)
[Publications] Ikeda,M.: "Developmental profile and tissue localization of the protease responsible for vitellin degradation in the silkworm,Bombyx mori." J.Sci.Jpn.60. 178-185 (1991)
[Publications] Imai,K: "Isolation and structure of diapause hormone of the silkworm,Bombyx mori." Proc.Japan Acad.67Ser.B. 98-101 (1991)
[Publications] Fujiwara,Y.: "A larval serum protein of the silkworm,Bombyx mori:cDNA sequence and developmental specificity of the transcript." Insect Biochem.21. 735-742 (1991)
[Publications] Kajiura Z.: "Induction of metamorphosis of the methopreneinduced dauer larvae of the silkworm,Bombyx mori,by ecdysteroids." Comp.Biochem.Physiol.101A. 277-280 (1991)
[Publications] Kageyama,T.: "Purification and characterization of a cysteine proteinase from silkworm eggs." Eur.J.Biochem.193. 203-210 (1991)
[Publications] Kobayashi,H.: "Cyclic GMPーdependent protein phosphorylation in relation to muscarinic ganglionic transmission in mammals." J.Autonom.Nervous System.33. 162-163 (1991)
[Publications] Takahashi,S.Y.: "Phosphorylation of follicle proteins from the teleost,Oryzias latipes." Biomed.Res.12. 231-239 (1991)
[Publications] Sugiyama,H: "A novel mechanismof Sarcophaga lectin gene expression" Euro J.Biochem.200. 495-500 (1991)
[Publications] Yonemura,M.: "Involvement of a serine protease in the activation of prophenoloxidase in Drosophila melanogaster." Zool.Sci.8. 865-867 (1991)
[Publications] Yamasaki,H.: "Activation of laccaseーtype proーphenoloxidse in the cuticle of insect.VIII Prolaccase in larval cuticle of silkworm,Bombyx mori." Zool.Sci.8. 1144 (1991)
[Publications] Agai,N.: "Effect of juvenile hormone analogue on ovarian development of the reprodactiveーdiapause parasitic fly,Melinda pusilla." Jpn.J.Sanit Zool. 42. 311-317 (1991)
[Publications] "Use of nitrocellulose membrane to activate and measure insect phenoloxidase." Anal.Biochem.194. 359-364 (1991)
[Publications] Kim,J.W.: "Purification of stageーspecific and sequencespecific DNAーbinding protein for the arylphorin gene of sarcophagaperegrina." Biochim.Biophys Acta. 1089. 21-26 (1991)
[Publications] Jomori,T.: "Molecular cloning of cDNA for lipopolysaccharidebinding protein from the hemolymph of the American cockroach,Periplaneta americana." J.Biol.Chem.266. 13318-13323 (1991)
[Publications] Komano,H.: "Involvement of sapein in embryonic cell prolification of Sarcophaga peregrina." FEBS Letts.289. 167-170 (1991)

1991 Fiscal Year Annual Research Report

昆虫後期発生におけるタンパク質生合成ならびに機能発現調節機構

Principal Investigator

富野 士良 東京都立大学, 理学部, 教授 (30101075)

Research Products

[Publications] Mori,S.: "Structure and organization of genes for major plasma proteins of Bombyx mori." J.Mol.Biol.218. 7-12 (1991)

[Publications] Mori,S.: "Complete nucleotide sequence of major plasma protein genes of Bombyx mori." Biochim.Biophys.Acta. 1090. 129-132 (1991)

[Publications] Agui,N: "Hormonal control of vitellogenin mRNA level in the male andfemale housefly,Musca domestica." J.Insect Physiol.37. 383-390 (1991)

[Publications] Itoh,M.: "Cloning and sequence analysis of membrane bound alkaline phosphaase cDNA of the silkworm,Bombyx mori." Biochim.Biophys.Acta. 1129. 135-138 (1991)

[Publications] Yaginuma.T.: "Does an enzyme activity capable of phosphorylating sorbitol control utilization of sorbitol at the termination of diapause in eggs of the silkworm Bombyx mori." Comp.Biochem.Biophys.98B. 135-141 (1991)

[Publications] Sato,Y.: "Synthesis and secretion of eggーspecific protein from follicle cells of the silkworm,Bombyx mori." Insect Biochem.21. 233-238 (1991)

[Publications] Ikeda,M.: "cDNA cloning,sequencing and temporal expression of the protease responsible for vitellin degradation in the silkworm,Bombyx mori." Comp.Biochem.Pysiol.99B. 405-411 (1991)

[Publications] Ikeda,M.: "Developmental profile and tissue localization of the protease responsible for vitellin degradation in the silkworm,Bombyx mori." J.Sci.Jpn.60. 178-185 (1991)

[Publications] Imai,K: "Isolation and structure of diapause hormone of the silkworm,Bombyx mori." Proc.Japan Acad.67Ser.B. 98-101 (1991)

[Publications] Fujiwara,Y.: "A larval serum protein of the silkworm,Bombyx mori:cDNA sequence and developmental specificity of the transcript." Insect Biochem.21. 735-742 (1991)

[Publications] Kajiura Z.: "Induction of metamorphosis of the methopreneinduced dauer larvae of the silkworm,Bombyx mori,by ecdysteroids." Comp.Biochem.Physiol.101A. 277-280 (1991)

[Publications] Kageyama,T.: "Purification and characterization of a cysteine proteinase from silkworm eggs." Eur.J.Biochem.193. 203-210 (1991)

[Publications] Kobayashi,H.: "Cyclic GMPーdependent protein phosphorylation in relation to muscarinic ganglionic transmission in mammals." J.Autonom.Nervous System.33. 162-163 (1991)

[Publications] Takahashi,S.Y.: "Phosphorylation of follicle proteins from the teleost,Oryzias latipes." Biomed.Res.12. 231-239 (1991)

[Publications] Sugiyama,H: "A novel mechanismof Sarcophaga lectin gene expression" Euro J.Biochem.200. 495-500 (1991)

[Publications] Yonemura,M.: "Involvement of a serine protease in the activation of prophenoloxidase in Drosophila melanogaster." Zool.Sci.8. 865-867 (1991)

[Publications] Yamasaki,H.: "Activation of laccaseーtype proーphenoloxidse in the cuticle of insect.VIII Prolaccase in larval cuticle of silkworm,Bombyx mori." Zool.Sci.8. 1144 (1991)

[Publications] Agai,N.: "Effect of juvenile hormone analogue on ovarian development of the reprodactiveーdiapause parasitic fly,Melinda pusilla." Jpn.J.Sanit Zool. 42. 311-317 (1991)

[Publications] "Use of nitrocellulose membrane to activate and measure insect phenoloxidase." Anal.Biochem.194. 359-364 (1991)

[Publications] Kim,J.W.: "Purification of stageーspecific and sequencespecific DNAーbinding protein for the arylphorin gene of sarcophagaperegrina." Biochim.Biophys Acta. 1089. 21-26 (1991)

[Publications] Jomori,T.: "Molecular cloning of cDNA for lipopolysaccharidebinding protein from the hemolymph of the American cockroach,Periplaneta americana." J.Biol.Chem.266. 13318-13323 (1991)

[Publications] Komano,H.: "Involvement of sapein in embryonic cell prolification of Sarcophaga peregrina." FEBS Letts.289. 167-170 (1991)

富野士良東京都立大学, 理学部, 教授 (30101075)