1990 Fiscal Year Annual Research Report

タンパク質工学の基盤確立のための多角的アプロ-チ

Research Project

Project/Area Number	02304062
Research Institution	Kyushu University
Principal Investigator	井本泰治九州大学, 薬学部, 教授 (90038282)
Co-Investigator(Kenkyū-buntansha)	崎山文夫大阪大学, 蛋白質研究所, 教授 (40029947) 大塚栄子北海道大学, 薬学部, 教授 (80028836) 太田隆久東京大学, 農学部, 教授 (30011844) 岩永貞昭九州大学, 理学部, 教授 (90029942) 芦田玉一名古屋大学, 工学部, 教授 (10029936)
Keywords	タンパク質工学 / 核酸修復酵素 / Lー乳酸脱水素酵素 / ATPase / キメラタンパク質 / モジュ-ル / Znープロテア-ゼ / ボ-マン・バ-ク型インヒビタ-
Research Abstract	タンパク質工学の基盤を確立する目的で相互に密な連絡を取りながら以下の研究を行った。3種の奄美ハブ毒ホスホリパ-ゼA_2のcDNAを調製した(大野)。IX因子及びVII因子、Protein Zに発見した新規の糖鎖の構造を決定すると共に、同じ糖鎖がスロンボスポシジンにも含まれることを明らかにした(岩永)。ヒト筋型及び肝型アルドラ-ゼのキメラ型酵素を大腸菌で発現させ、活性をもつ酵素を7種類精製した(堀)。T4エンドヌクレア-ゼVの変異酵素をいくつか調製し、合成基質との反応を行った(大塚)。B__ー.<stearothermophilus>___ーのリジルtRNA合成酵素とLーリジン及びATPの結合を解析した(外村)。H^+・ATPaseの活性にはThr156が重要であることを明らかにした(二井)。<Bacillus>___ー <subtilis>___ーの生産する中性プロテア-ゼ(金属酵素)の全一次構造を決定し、活性発現に関与する残基及びZnに配位する残基を明らかにした(鶴)。部位特異的変異により、APIの基質特異性決定基はGlu190ではなくAsp225であることを明かにした(崎山)。遺伝性NADHーシトクロムb_5還元酵素異常症の遺伝子変異(Ser127→Pro)を同定し、同変異酵素の性質を調べた(指吸)。TIMバレル型タンパク質のモジュ-ル構成と機能の相関を明らかにした(郷)。ボ-マン・バ-ク型インヒビタ-の構造をピ-ナッツとミドリマメについて比較し、構造と活性の関係を調べた(小林)。ボ-マン・バ-ク型インヒビタ-の2.3A分解能のX線結晶解析を完了した(芦田)。Lー乳酸脱水素酵素のサブユニットの電荷を変化させることにより、サブユニット間相互作用を調べた(太田)。蛋白質へ糖鎖を導入する新しい方法を開発し、それがリゾチ-ムの熱安定性の改善に有効であることを示した(山崎)。大腸菌に発現させたニワトリリゾチ-ムの試験管内でのN末端のプロセッシングと巻戻しのシステムを開発した(井本)。

Research Products

(45 results)

All Other

All Publications (45 results)

[Publications] Kitajima,Takasaki,Takahasi,& Hori: "Construction and properties of active chimeric enzymes between human aldolases A and B: Analysis of molecular regions which determine isozymeーspecific functions." J.Biol.Chem.265. 17493-17498 (1990)
[Publications] Takasaki,Takahasi,Mukai & Hori: "Human aldolase A of a hemolytic anemia patient with Aspー128ーGly substitution: Characterizations of an enzyme generated in E__ー.<coli>___ー transfected with the expression plasmid pHAAD128G." J.Biochem.108. 153-157 (1990)
[Publications] Takasaki,& Hori: "Construction of rat aldolase C expression plasmid and the hybrid formation between rat aldlase C and human aldolase A or B coーexpressed in <Eschericia>___ー <coli>___ー." Protein Engineering. 3. 273-277 (1990)
[Publications] Kitajima,Matsuhashi,Nishida,Takasaki,TAkahashi,Hisatsugu,& Hori: "Monoclonal antibodies recognizing aminoーterminal and carboxyーterminal regions of human aldolase A: Probes to detect conformational change of the enzyme." J.Biochem.
[Publications] Ishida,Kanamori,Hori,Inaoka,& Otsuka: "In vitro and in vivo activities of T4 endonuclease V mutants altered in the Cーterminal aromatic region." Biochemistry. 29. 3817-3821 (1990)
[Publications] Murata,Iwai,& Otsuka: "Synthesis and chracterization of a substance for T4 endonuclease V containing a phosphorodithioate linkage at the thimine dimer site." Nucleic Acid Reserch. 18. 7279-7286 (1990)
[Publications] Sueyosi T.,et al.: "Cysteine proteinase inhibitor in the ascitric fluid of sarcoma 180 tumorーbearing mice is a lowーmolecularーweight kininogen: Partial COOHーterminal sequence and susceptibility to various glandular kallikrein." J.Biol.Chem.265. 10030-10035 (1990)
[Publications] Hase,S.,et al.: "Structure of (Xyl)2Glcー0ーserー53 found in the first epidermal growth factorーlike domain of bovine blood clotting factor IX." J.Biol.Chem.265. 1858-1861 (1990)
[Publications] Takeya,H.,et al.: "The complete amino acid sequence of the high molecular Mass hemorrhagic protein,HR1B,isolated from the venom of <Trimeresurus>___ー <flavoviridis>___ー." J.Biol.Chem.265. 16068-16073 (1990)
[Publications] Kawano,K.,et al.: "Antimicrobial peptide,tachyplesin I,isolated from hemocytes of the horseshoe crab (<Tachypleus>___ー <tridentatus>___ー): NMR determination of the βーsheet structure." J.Biol.Chem.265. 15365-15367 (1990)
[Publications] Shigenaga,T.,et al.: "Antimicrobial tachyplesin peptide precursor: cDNA cloning and celluar localization in horseshoe crab (<Tachypleus>___ー <tridentatus>___ー)" J.Biol.Chem.265. 21350-21354 (1990)
[Publications] Takeya,H.,et al.: "Primary structure of a hemorrhagic metalloproteinase,HTー2,isolated from the venom of <Crotalus>___ー <ruber>___ー <ruber>___ー." J.Biochem.108. 711-719 (1990)
[Publications] Yoahimoto,Oyama,Takeshita,Higashi,Xu,& Tsuru: "Nucleotide sequence of the neutral protease gene from <Bacillus>___ー <subtilis>___ー <var>___ー.<amylosacchariticus>___ー." J.Ferment.Bioeng.70. 370-375 (1990)
[Publications] 鶴大典: "Bacillus属最近の分泌するプロテア-ゼの比較生化学" 日本農芸化学会誌. 65. 50-55 (1990)
[Publications] Go,M.,et al.: "Protein modules: Structural characterization of modules barnase in solution"
[Publications] Takagi,Omura,& Go: "Evolutionary origin of thyroglobulin by duplication of esterase gene."
[Publications] Go,Tomoda,Honda,& Yutani: "Domain and module structure in α/βーbarrel of tryptophan synthase α subunit."
[Publications] Yoshizumi,Ryu,Miyata,Saida,Ohno,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein I,a lysineー49ーphospholipase A2 with low activity,from the venom of <Trimeresurus>___ー <flavoridis>___ー (Habu snake)." Toxicon. 28. 45-54 (1990)
[Publications] Shinoda,Shoji,Yamane,Suzuki,Ashida,Kihara,& Ohno: "Preliminary crystallographic study of phospholipase A2 from the venom of <Trimeresurus>___ー <flavoridis>___ー (Habu snake)." J.Biochem.107. 84-86 (1990)
[Publications] Ryu,Yoshizumi,Oda,Ohno,Tokunaga,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein II,a lysineー49ーphospholipase A2 with low activity,from <Trimeresurus>___ー <flavoridis>___ー venom." J.Biochem.107. 400-408 (1990)
[Publications] Oda,Ogawa,Ohno,Sasaki,Sakaki,& Kihara: "Cloning and sequence analysis of cDNA for <Trimeresurus>___ー <flavoviridis>___ー phospholipase A2,and consequent revision of the amino acid sequence." J.Biochem.108. 816-821 (1990)
[Publications] Maeda & Futai: "Structural analysis of gastric (H^++K^+)ーATPase." Ann.N.Y.Acad.Sci.402. 204-207 (1990)
[Publications] Iwamoto,Miki,Maeda,& Futai: "H^+ーATPase γ subunit from <Eschericia>___ー <coli>___ー: Role of the conserved carboxy terminal region." J.Biol.Chem.265. 5043-5048 (1990)
[Publications] Maeda,Oshiman,Tamura,& Futai: "Human gastric (H^++K^+)ーATPase gene: Similarity to (H^++K^+)ーATPase genes in exon/intron organization but difference in control region." J.Biol.Chem.265. 9027-9032 (1990)
[Publications] Suzuki,Ashida,Kihara,& Ohno: "The cytotoxic action of diphtheria toxin and its degradation in intact Vero cells are inhibited by bafilomycin A1,a specific inhibitot of vacuolarーtype H^+ーATPase." J.Biol.Chem.265. 21940-21945 (1990)
[Publications] Takeyama,Ihara,Moriyama,Noumi,Ida,Tomochika,itai,Maeda,& Futai: "The glycineーrich sequence of the β subunit of <Eschericia>___ー <coli>___ー H^+ーATPase is i,portant for activity." J.Biol.Chem.265. 21279-21284 (1990)
[Publications] Yamasaki,Eto,Abe,& Yamashita: "<Fluorescene>___ー spectroscopic properties of an active lysozyme derivative: Kyn62ーlysozyme." Agric Biol.Chem.54. 1883-1884 (1990)
[Publications] Yamasaki,Matsuo,Hatakeyama,& Funatsu: "Some properties of ricin D modified with methoxypolyethylene glycol derivative." Agric Biol.Chem.54. 2635-2640 (1990)
[Publications] Kobayashi,Y.,et al.: "Serineーproline replacement at residue 127 of NADHーcytochrome b_5 reductase causes hereditary methemologbinemia,generalized type." Blood. 75. 1408-1413 (1990)
[Publications] Zenno,S.,et al.: "Molecular cloning of a cDNA encoding rat NADHーcytochrome b_5 reductase and the corresponding gene." J.Biochem.107. 810-816 (1990)
[Publications] Yubisui,T.,et al.: "Rapid reduction of methemoglobin in rat bone marrow erythroid cells." Amer.J.Hematology. 34. 5-7 (1990)
[Publications] Yubisui,T.,et al.: "Structural role of Serine 127 in the NADHーbinding site of human NADHーcytochrome b_5 reductase." J.Biol.Chem.266. 66-70 (1991)
[Publications] Ono,Matsuzawa,& Ohta: "Nucleotide sequence and characteristics of the gene for Lーlactate dehydrogenase of <Thermaus>___ー <aquaticus>___ー YTー1 and the deduced amino acid sequence of the enzyme." J.Biochem.107. 21-26 (1990)
[Publications] Koide,Iwata,Matsuzawa,& Ohta: "Crystallization of allosteric Lーlactate dehydrogenase from <Thermus>___ー <caldophilus>___ー and preliminary crystallographic data." J.Biochem.109. 6-7 (1991)
[Publications] Suzuki,A.,et al.: "Crystallization and preliminary crystallographic study of bacterial αーamylase." J.Biochem.108. 379-381 (1990)
[Publications] Suzuki,A.,et al.: "Crystalーstructure of BowmanーBirk inhibitor AII from peanut at 2.3 A resolution." J.Biochem.
[Publications] Ito,Yamada,Nakamura,& Imoto: "Purification,amino acid sequence and some properties of rabbit kidney lysozyme." J.Biochem.107. 236-241 (1990)
[Publications] Yamada,Yamashita,Domoto,& Imoto: "Reaction of hen eggーwhite lysozyme with tetranitromethane: A new side reaction,oxidative bond cleavage at glycine 104,and sequential nitration of three tyrosine residues." J.Biochem.108. 432-440 (1990)
[Publications] Ueda,Yamada,Aoki,& Imoto: "Effect of chemical modifica<tions of>___ー <tryptopha>___ーn residues on the folding of reduced hen eggーwhite lysozyme." J.Biochem.108. 886-892 (1990)
[Publications] Koga,Yamada,Nishimura,Kato,& Imoto: "Comparative study on specificities of rat cathepsin L and papain: Amino acid differences at substrateーbinding sites are involved in their specificities." J.Biochem.108. 976-982 (1990)
[Publications] Takasaki,Kitajima,Takahashi,Sakakibara,Mukai,& Hori: "Structural studies on aldolase isozymes through protein engineering In ISOZYMES: Structure,Function,and Use in Biology and Medicine." Ogita,Z.,& Markert,C.L.(eds.) WileyーLiss,Inc.New York, 19 (1990)
[Publications] 宮田敏行、岩永貞昭: "研究実験講座 XIII 臨床検査法の最前線II.6,凝固因子の分子異常症の検出" 中山書店(代謝27巻、第6号), 14 (1990)
[Publications] 西村仁、岩永貞昭: "臨床科学8,内皮細胞シリ-ズ、26巻第3号" 世界保健通信社, 14 (1990)
[Publications] 鶴大典: "プロテア-ゼとそのインヒビタ-(分担)(金属プロテア-ゼとそのインヒビタ-)" メジカルレビュ-社, 20 (1990)
[Publications] Michiko Go: "In <Evolution>___ー <of>___ー <Life>___ー,ed.S.Osawa“Module organization in proteins and exon Shuffling"" SpringerーVerlag,

1990 Fiscal Year Annual Research Report

タンパク質工学の基盤確立のための多角的アプロ-チ

Principal Investigator

井本 泰治 九州大学, 薬学部, 教授 (90038282)

Research Products

[Publications] Kitajima,Takasaki,Takahasi,& Hori: "Construction and properties of active chimeric enzymes between human aldolases A and B: Analysis of molecular regions which determine isozymeーspecific functions." J.Biol.Chem.265. 17493-17498 (1990)

[Publications] Takasaki,Takahasi,Mukai & Hori: "Human aldolase A of a hemolytic anemia patient with Aspー128ーGly substitution: Characterizations of an enzyme generated in E__ー.<coli>___ー transfected with the expression plasmid pHAAD128G." J.Biochem.108. 153-157 (1990)

[Publications] Takasaki,& Hori: "Construction of rat aldolase C expression plasmid and the hybrid formation between rat aldlase C and human aldolase A or B coーexpressed in <Eschericia>___ー <coli>___ー." Protein Engineering. 3. 273-277 (1990)

[Publications] Kitajima,Matsuhashi,Nishida,Takasaki,TAkahashi,Hisatsugu,& Hori: "Monoclonal antibodies recognizing aminoーterminal and carboxyーterminal regions of human aldolase A: Probes to detect conformational change of the enzyme." J.Biochem.

[Publications] Ishida,Kanamori,Hori,Inaoka,& Otsuka: "In vitro and in vivo activities of T4 endonuclease V mutants altered in the Cーterminal aromatic region." Biochemistry. 29. 3817-3821 (1990)

[Publications] Murata,Iwai,& Otsuka: "Synthesis and chracterization of a substance for T4 endonuclease V containing a phosphorodithioate linkage at the thimine dimer site." Nucleic Acid Reserch. 18. 7279-7286 (1990)

[Publications] Sueyosi T.,et al.: "Cysteine proteinase inhibitor in the ascitric fluid of sarcoma 180 tumorーbearing mice is a lowーmolecularーweight kininogen: Partial COOHーterminal sequence and susceptibility to various glandular kallikrein." J.Biol.Chem.265. 10030-10035 (1990)

[Publications] Hase,S.,et al.: "Structure of (Xyl)2Glcー0ーserー53 found in the first epidermal growth factorーlike domain of bovine blood clotting factor IX." J.Biol.Chem.265. 1858-1861 (1990)

[Publications] Takeya,H.,et al.: "The complete amino acid sequence of the high molecular Mass hemorrhagic protein,HR1B,isolated from the venom of <Trimeresurus>___ー <flavoviridis>___ー." J.Biol.Chem.265. 16068-16073 (1990)

[Publications] Kawano,K.,et al.: "Antimicrobial peptide,tachyplesin I,isolated from hemocytes of the horseshoe crab (<Tachypleus>___ー <tridentatus>___ー): NMR determination of the βーsheet structure." J.Biol.Chem.265. 15365-15367 (1990)

[Publications] Shigenaga,T.,et al.: "Antimicrobial tachyplesin peptide precursor: cDNA cloning and celluar localization in horseshoe crab (<Tachypleus>___ー <tridentatus>___ー)" J.Biol.Chem.265. 21350-21354 (1990)

[Publications] Takeya,H.,et al.: "Primary structure of a hemorrhagic metalloproteinase,HTー2,isolated from the venom of <Crotalus>___ー <ruber>___ー <ruber>___ー." J.Biochem.108. 711-719 (1990)

[Publications] Yoahimoto,Oyama,Takeshita,Higashi,Xu,& Tsuru: "Nucleotide sequence of the neutral protease gene from <Bacillus>___ー <subtilis>___ー <var>___ー.<amylosacchariticus>___ー." J.Ferment.Bioeng.70. 370-375 (1990)

[Publications] 鶴 大典: "Bacillus属最近の分泌するプロテア-ゼの比較生化学" 日本農芸化学会誌. 65. 50-55 (1990)

[Publications] Go,M.,et al.: "Protein modules: Structural characterization of modules barnase in solution"

[Publications] Takagi,Omura,& Go: "Evolutionary origin of thyroglobulin by duplication of esterase gene."

[Publications] Go,Tomoda,Honda,& Yutani: "Domain and module structure in α/βーbarrel of tryptophan synthase α subunit."

[Publications] Yoshizumi,Ryu,Miyata,Saida,Ohno,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein I,a lysineー49ーphospholipase A2 with low activity,from the venom of <Trimeresurus>___ー <flavoridis>___ー (Habu snake)." Toxicon. 28. 45-54 (1990)

[Publications] Shinoda,Shoji,Yamane,Suzuki,Ashida,Kihara,& Ohno: "Preliminary crystallographic study of phospholipase A2 from the venom of <Trimeresurus>___ー <flavoridis>___ー (Habu snake)." J.Biochem.107. 84-86 (1990)

[Publications] Ryu,Yoshizumi,Oda,Ohno,Tokunaga,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein II,a lysineー49ーphospholipase A2 with low activity,from <Trimeresurus>___ー <flavoridis>___ー venom." J.Biochem.107. 400-408 (1990)

[Publications] Oda,Ogawa,Ohno,Sasaki,Sakaki,& Kihara: "Cloning and sequence analysis of cDNA for <Trimeresurus>___ー <flavoviridis>___ー phospholipase A2,and consequent revision of the amino acid sequence." J.Biochem.108. 816-821 (1990)

[Publications] Maeda & Futai: "Structural analysis of gastric (H^++K^+)ーATPase." Ann.N.Y.Acad.Sci.402. 204-207 (1990)

[Publications] Iwamoto,Miki,Maeda,& Futai: "H^+ーATPase γ subunit from <Eschericia>___ー <coli>___ー: Role of the conserved carboxy terminal region." J.Biol.Chem.265. 5043-5048 (1990)

[Publications] Maeda,Oshiman,Tamura,& Futai: "Human gastric (H^++K^+)ーATPase gene: Similarity to (H^++K^+)ーATPase genes in exon/intron organization but difference in control region." J.Biol.Chem.265. 9027-9032 (1990)

[Publications] Suzuki,Ashida,Kihara,& Ohno: "The cytotoxic action of diphtheria toxin and its degradation in intact Vero cells are inhibited by bafilomycin A1,a specific inhibitot of vacuolarーtype H^+ーATPase." J.Biol.Chem.265. 21940-21945 (1990)

[Publications] Takeyama,Ihara,Moriyama,Noumi,Ida,Tomochika,itai,Maeda,& Futai: "The glycineーrich sequence of the β subunit of <Eschericia>___ー <coli>___ー H^+ーATPase is i,portant for activity." J.Biol.Chem.265. 21279-21284 (1990)

[Publications] Yamasaki,Eto,Abe,& Yamashita: "<Fluorescene>___ー spectroscopic properties of an active lysozyme derivative: Kyn62ーlysozyme." Agric Biol.Chem.54. 1883-1884 (1990)

[Publications] Yamasaki,Matsuo,Hatakeyama,& Funatsu: "Some properties of ricin D modified with methoxypolyethylene glycol derivative." Agric Biol.Chem.54. 2635-2640 (1990)

[Publications] Kobayashi,Y.,et al.: "Serineーproline replacement at residue 127 of NADHーcytochrome b_5 reductase causes hereditary methemologbinemia,generalized type." Blood. 75. 1408-1413 (1990)

[Publications] Zenno,S.,et al.: "Molecular cloning of a cDNA encoding rat NADHーcytochrome b_5 reductase and the corresponding gene." J.Biochem.107. 810-816 (1990)

[Publications] Yubisui,T.,et al.: "Rapid reduction of methemoglobin in rat bone marrow erythroid cells." Amer.J.Hematology. 34. 5-7 (1990)

[Publications] Yubisui,T.,et al.: "Structural role of Serine 127 in the NADHーbinding site of human NADHーcytochrome b_5 reductase." J.Biol.Chem.266. 66-70 (1991)

[Publications] Ono,Matsuzawa,& Ohta: "Nucleotide sequence and characteristics of the gene for Lーlactate dehydrogenase of <Thermaus>___ー <aquaticus>___ー YTー1 and the deduced amino acid sequence of the enzyme." J.Biochem.107. 21-26 (1990)

[Publications] Koide,Iwata,Matsuzawa,& Ohta: "Crystallization of allosteric Lーlactate dehydrogenase from <Thermus>___ー <caldophilus>___ー and preliminary crystallographic data." J.Biochem.109. 6-7 (1991)

[Publications] Suzuki,A.,et al.: "Crystallization and preliminary crystallographic study of bacterial αーamylase." J.Biochem.108. 379-381 (1990)

[Publications] Suzuki,A.,et al.: "Crystalーstructure of BowmanーBirk inhibitor AII from peanut at 2.3 A resolution." J.Biochem.

[Publications] Ito,Yamada,Nakamura,& Imoto: "Purification,amino acid sequence and some properties of rabbit kidney lysozyme." J.Biochem.107. 236-241 (1990)

[Publications] Yamada,Yamashita,Domoto,& Imoto: "Reaction of hen eggーwhite lysozyme with tetranitromethane: A new side reaction,oxidative bond cleavage at glycine 104,and sequential nitration of three tyrosine residues." J.Biochem.108. 432-440 (1990)

[Publications] Ueda,Yamada,Aoki,& Imoto: "Effect of chemical modifica<tions of>___ー <tryptopha>___ーn residues on the folding of reduced hen eggーwhite lysozyme." J.Biochem.108. 886-892 (1990)

[Publications] Koga,Yamada,Nishimura,Kato,& Imoto: "Comparative study on specificities of rat cathepsin L and papain: Amino acid differences at substrateーbinding sites are involved in their specificities." J.Biochem.108. 976-982 (1990)

[Publications] Takasaki,Kitajima,Takahashi,Sakakibara,Mukai,& Hori: "Structural studies on aldolase isozymes through protein engineering In ISOZYMES: Structure,Function,and Use in Biology and Medicine." Ogita,Z.,& Markert,C.L.(eds.) WileyーLiss,Inc.New York, 19 (1990)

[Publications] 宮田 敏行、岩永 貞昭: "研究実験講座 XIII 臨床検査法の最前線II.6,凝固因子の分子異常症の検出" 中山書店(代謝27巻、第6号), 14 (1990)

[Publications] 西村 仁、岩永 貞昭: "臨床科学8,内皮細胞シリ-ズ、26巻第3号" 世界保健通信社, 14 (1990)

[Publications] 鶴 大典: "プロテア-ゼとそのインヒビタ-(分担)(金属プロテア-ゼとそのインヒビタ-)" メジカルレビュ-社, 20 (1990)

[Publications] Michiko Go: "In <Evolution>___ー <of>___ー <Life>___ー,ed.S.Osawa“Module organization in proteins and exon Shuffling"" SpringerーVerlag,

井本泰治九州大学, 薬学部, 教授 (90038282)

[Publications] Takasaki,Takahasi,Mukai & Hori: "Human aldolase A of a hemolytic anemia patient with Aspー128ーGly substitution: Characterizations of an enzyme generated in Eー.<coli>_ー transfected with the expression plasmid pHAAD128G." J.Biochem.108. 153-157 (1990)

[Publications] Takasaki,& Hori: "Construction of rat aldolase C expression plasmid and the hybrid formation between rat aldlase C and human aldolase A or B coーexpressed in <Eschericia>_ー <coli>_ー." Protein Engineering. 3. 273-277 (1990)

[Publications] Takeya,H.,et al.: "The complete amino acid sequence of the high molecular Mass hemorrhagic protein,HR1B,isolated from the venom of <Trimeresurus>_ー <flavoviridis>_ー." J.Biol.Chem.265. 16068-16073 (1990)

[Publications] Kawano,K.,et al.: "Antimicrobial peptide,tachyplesin I,isolated from hemocytes of the horseshoe crab (<Tachypleus>_ー <tridentatus>_ー): NMR determination of the βーsheet structure." J.Biol.Chem.265. 15365-15367 (1990)

[Publications] Shigenaga,T.,et al.: "Antimicrobial tachyplesin peptide precursor: cDNA cloning and celluar localization in horseshoe crab (<Tachypleus>_ー <tridentatus>_ー)" J.Biol.Chem.265. 21350-21354 (1990)

[Publications] Takeya,H.,et al.: "Primary structure of a hemorrhagic metalloproteinase,HTー2,isolated from the venom of <Crotalus>_ー <ruber>_ー <ruber>___ー." J.Biochem.108. 711-719 (1990)

[Publications] Yoahimoto,Oyama,Takeshita,Higashi,Xu,& Tsuru: "Nucleotide sequence of the neutral protease gene from <Bacillus>_ー <subtilis>_ー <var>_ー.<amylosacchariticus>_ー." J.Ferment.Bioeng.70. 370-375 (1990)

[Publications] 鶴大典: "Bacillus属最近の分泌するプロテア-ゼの比較生化学" 日本農芸化学会誌. 65. 50-55 (1990)

[Publications] Yoshizumi,Ryu,Miyata,Saida,Ohno,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein I,a lysineー49ーphospholipase A2 with low activity,from the venom of <Trimeresurus>_ー <flavoridis>_ー (Habu snake)." Toxicon. 28. 45-54 (1990)

[Publications] Shinoda,Shoji,Yamane,Suzuki,Ashida,Kihara,& Ohno: "Preliminary crystallographic study of phospholipase A2 from the venom of <Trimeresurus>_ー <flavoridis>_ー (Habu snake)." J.Biochem.107. 84-86 (1990)

[Publications] Ryu,Yoshizumi,Oda,Ohno,Tokunaga,Iwanaga,& Kihara: "Purification and amino acid sequence of basic protein II,a lysineー49ーphospholipase A2 with low activity,from <Trimeresurus>_ー <flavoridis>_ー venom." J.Biochem.107. 400-408 (1990)

[Publications] Oda,Ogawa,Ohno,Sasaki,Sakaki,& Kihara: "Cloning and sequence analysis of cDNA for <Trimeresurus>_ー <flavoviridis>_ー phospholipase A2,and consequent revision of the amino acid sequence." J.Biochem.108. 816-821 (1990)

[Publications] Iwamoto,Miki,Maeda,& Futai: "H^+ーATPase γ subunit from <Eschericia>_ー <coli>_ー: Role of the conserved carboxy terminal region." J.Biol.Chem.265. 5043-5048 (1990)

[Publications] Takeyama,Ihara,Moriyama,Noumi,Ida,Tomochika,itai,Maeda,& Futai: "The glycineーrich sequence of the β subunit of <Eschericia>_ー <coli>_ー H^+ーATPase is i,portant for activity." J.Biol.Chem.265. 21279-21284 (1990)

[Publications] Ono,Matsuzawa,& Ohta: "Nucleotide sequence and characteristics of the gene for Lーlactate dehydrogenase of <Thermaus>_ー <aquaticus>_ー YTー1 and the deduced amino acid sequence of the enzyme." J.Biochem.107. 21-26 (1990)

[Publications] Koide,Iwata,Matsuzawa,& Ohta: "Crystallization of allosteric Lーlactate dehydrogenase from <Thermus>_ー <caldophilus>_ー and preliminary crystallographic data." J.Biochem.109. 6-7 (1991)

[Publications] Ueda,Yamada,Aoki,& Imoto: "Effect of chemical modifica<tions of>_ー <tryptopha>_ーn residues on the folding of reduced hen eggーwhite lysozyme." J.Biochem.108. 886-892 (1990)

[Publications] 宮田敏行、岩永貞昭: "研究実験講座 XIII 臨床検査法の最前線II.6,凝固因子の分子異常症の検出" 中山書店(代謝27巻、第6号), 14 (1990)

[Publications] 西村仁、岩永貞昭: "臨床科学8,内皮細胞シリ-ズ、26巻第3号" 世界保健通信社, 14 (1990)

[Publications] 鶴大典: "プロテア-ゼとそのインヒビタ-(分担)(金属プロテア-ゼとそのインヒビタ-)" メジカルレビュ-社, 20 (1990)

[Publications] Michiko Go: "In <Evolution>_ー <of>_ー <Life>___ー,ed.S.Osawa“Module organization in proteins and exon Shuffling"" SpringerーVerlag,