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1991 Fiscal Year Final Research Report Summary

Analysis of Functional Membrane Proteins by Planar Phospholiped Bilayers.

Research Project

Project/Area Number 02304067
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionHimeji Institute of Technology, Faculty of Science (1991)
Jichi Medical University (1990)

Principal Investigator

HIRATA Hajime  Himeji Inst. Tech., Faculty Sci., Prof., 理学部, 教授 (40049052)

Co-Investigator(Kenkyū-buntansha) TAKAGI Masayuki  Osaka Univ., Educ. Sch., Assoc. Prof., 教養部, 助教授 (20028145)
SOKABE Masahiro  Nagoya Univ., Med. Sch., Assoc. Prof., 医学部, 助教授 (10093428)
TSUDA Motoyuki  Himeji Inst. Tech., Faculty Sci., Prof., 理学部, 教授 (60045458)
KIRINO Yutaka  Kyushu Univ., Faculty Pharm., Prof., 薬学部, 教授 (10012668)
KASAI Michiki  Osaka Univ., Faculty Eng. Sci., Prof., 基礎工学部, 教授 (40022595)
Project Period (FY) 1990 – 1991
KeywordsPhospholipid Planar Bilayer / Ion Channel / Ion Pump / Signal Transduction / Functional Membrane Protein / Synthetic Peptide / Cryo-Electron Microscopy / Computer Simulation
Research Abstract

1) The Cl^--channels in synaptic vesicle membranes were characterized after reconstituting into planar bilayers and classified into two types of activities. It was also demonstrated that fusion of synaptic vesicles with liposomes was triggered by soiie unknown protein factor (s). 2) A synthetic polyether in combination with quaternary amine compound formed a K^+ specific ion channel in planar bilayers. Chemical modification of gramicidin as well as synthetic peptides was introduced for characterization of ion permeation through ion channel molecules. 3) Amphiphilic peptides composed of 20 amino acids from N-terminal segment of influenza virus were synthesized and their membrane fusion acitvities were characterized. 4) Using planar bilayers as an assay procedure, K^+-channel and Cl^--channel proteins were purified from sarcoplasmic reticulum membranes. 5) Ion channel activities in giant liposomes prepared by various methods were characterized in detail comparing with those in planar bilayers. 6) A new method, a pinching oil layer method, for reconstituting ion channel proteins was developed. 7) Detailed analyses on interaction between rhodopsin and G-protein in octopus photoreceptor cells was carried out. And a cGMP activated cation channel in octopus photoreceptor cells was identified using planar bilayers. 8) Reconstitution of either bacteriorhodopsin or H^+-ATPase into planar bilayers was successfully carried out by newly developed methods ; a direct insertion method or membrane fusion peptide method. 9) Electron microscopic studies on Cl^--channel of frog muscle spindle were carried out and its structure was deduced. 10) A new time resolution cryoelectron microscope c technique was developed for studies of conformational changes of proteins at time resolution of 5 ms. 11) Visualization of diffusion of phospholipid molecules in phospholipid bilayer membranes was performed by computer simulation, and permeation of oxygen molecules were analyzed.

  • Research Products

    (110 results)

All Other

All Publications (110 results)

  • [Publications] S.Oiki: "Bundles of amphipathic transmembrane α-helices as a structural motif for ion-conducting channel proteins:Studies on sodium channels and acetylcholine receptors." Proteins. 8. 226-236 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 老木 成稔: "ペプチドによるイオンチャネル機能の再構成:アセチルコリン受容体ポアの構造と機能" 膜. 16. 284-294 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takumi: "Alteration of channel activities and gating by mutations of slow Isk potassium channel."" J.Biol.Chem.266. 22192-22198 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Oiki: "A dipolar amino acid substitution induces voltage-dependent transitions between two stable conductance states in gramicidin channels." Biophys.J.62. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nunogaki,K.: "How to separate the H^+ and OH^- permeanbilities:Kinetics of intravesicular pH change after medium pH jump." J.Theor.Biol.144. 415-427 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 葛西 道生: "生体電気信号の発生機構" 学術月報. 43. 124-129 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okihara,K.: "Ca^<2+> and voltage-dependent and calmodulin-antagonist-sensitive anion channel in Chara plasmalemma:A patch clamp study." Plant Cell Physiol.32. 593-601 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ide,T.: "Purification of a Cl^--channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system." Biochem.Biophys.Res.Commun.176. 38-44 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ide,T.: "Purification of a K^+-Channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system." Biochim.Biophys.Acta.1067. 213-220 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 葛西 道生: "筋収縮の制御に関連したイオンチャネル" 麻酔と蘇生. 27. 153-161 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: ""A.Simple Method for the Preparation of an Amphiphilic sequential Polypeptide"," Macromolecules. 23,. 361-365 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: ""Channel Forming Activity of an Amphiphilic Sequential Polypeptide in a Cationic Bilayer Membrane"" Bull.Chem.Soc.Jpn.63,. 1916-1920 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: ""Interaction between L-Glutamic Acid Containing Polypeptide and Cu^<2+> ion on a Bilayer Membrane"," Sen-i Gakkaishi. 49,. 384-390 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: ""Interaction between an Anionic Amphiphilic Sequential Polypeptide and Anionic Bilayer Membrane"," Polymer J.23,. 15-22 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anzai,K.: ""Change in Intravesicular Volume of Liposomes by Freeze-Thawing Treatment as Studied by the ESR Stopped-Flow Technique"" Biochim.Biophys.Acta,. 1021,. 21-26 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishie,Y.: ""Measurement of Steady-State Ca-Pump Current Caused by Purified Ca-ATPase of Sarcoplasmic Reticulum Incorporated into a Planar Bilayer Lipid Membrane"." J.Biol.Chem.,. 265,. 2488-2491 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anzai,K.: ""Formation of Ion Channels by Synthetic Basic Peptides in Planar Lipid Bilayer Membrane"" Biochim.Biophys.Acta,. 1064,. 256-266 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirashima,N.: ""Comparative Electrophysiological Study of Reconstituted Membrane Preparations of the Potassium Channel of the Rabbit Skeletal Muscle Sarcoplasmic Reticulum"" Biochim.Biophys.Acta,. 1067,. 235-240 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Agawa,Y.: ""Interaction with Phospholipid Bilayers,Ion Channel Formation,and Antimicrobiral Activity of Basic Amphipathic α-Helical Peptide Having Various Chain Length"" J.Biol.Chem.,. 266. 20218-20222 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sokabe,M.: "The structure and dynamics of patch clamped membranes:a study by using differential interference light microscopy." J.Cell Biol.111. 599-606 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura,K.: "Aminoglycoside blockade of Ca-activated K channels from rat brain synaptosomal membranes incorporated into planar bilayers." J.Membr.Biol.115. 241-251 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sachs,F.: "Stretch activated ion channels and membrane mechanics." Neurosci.Res.12. s1-4 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujitsuka,C.: "Intramembrane particles and responses of sensory axon terminals during reinnervation of the frog muscle spindle." J.Neurocytol.19. 175-186 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: ""筋紡錘では機械的変形をどのように電気信号に変換しているか"" 膜. 8. 121-129 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: ""マルチ計測顕微鏡を用いた機械受容チャネルの研究"" 聴覚研究会資料. Hー90ー56. 1-6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sokabe,M.: "Electrophysiological analysis of structural aspects of voltage-dependent SR K channel." Comp.Biochem.Physiol.98C. 23-30 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sokabe,M.: "Quantitative videomicroscopy of patch clamped membranes:stress,strain,capacitance,and stretch channel activation." Biophys.J.59. 722-728 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura,K.: "Anion channels from rat brain synaptosomal membranes incorporated into planar bilayers." J.Membr.Biol.124. 53-62 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashino,E.: "Hair cell damage and recovery following chronic application of kanamycin in the chick cochlea." Hearing Res.52. 356-368 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: ""神経細胞におけるアナログとデジタル"" Computer Today. 9. 41-43 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: ""マルチ計測超高倍率顕微鏡の開発"" 比較生理生化学. 8. 22-31 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bowman,C.L.: "Mechanotransducing ion channeles in astrocytes." Brain Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashino,E.: "Hair cell regeneration in the adult budgerigar after kanamycin ototoxicity." Hearing Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sokabe,M.: "Effects of excision and cytochalasin on the viscoelastic properties of patch clamped membranes in heart and skeletal muscle cells." Biophys.J.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: "膜伸展によって活性化されるイオンチャネル" 心臓. 24. 327-337 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: "Kチャネル" Clinical Neuroscience.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,S.: "Conformation of Membrane Fusion-active 20-Residue Peptides with or without Lipid Bilayers.Implication of α-Helix Formation for Membrane Fusion." Biochemistry. 29. 6257-6264 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murata,M.: "Membrane Fusion Induced by Mutual Interaction of the Two Charge-reversed Amphiphilic Peptides at Neutral pH." J.Biol.Chem.266. 14353-14358 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murata,M.: "Modification of the N-Terminus of Membrane Fusion-active Peptides Blocks the Fusion Activity." Biochem.Biophys.Res.Commun.179. 1050-1055 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murata,M.: "pH-Dependent Membrane Fusion and Vesiculation of Phospholipid Large Unilamellar Vesicles Induced by Amphiphilic and Cationic Pepitides." Biochemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pasenkiewicz-Gierula,M.: "Rotational diffusion of a steroid molecule in phosphatidylcholine-cholesterol membranes:fluid phase microimmiscibility in unsaturated-phosphatidylcholine-cholesterol membranes." Biochemistry. 29. 4059-4069 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Subczynski,W.K.: "Microimmiscibility and three-dimensional structure of unsaturated phosphatidylcholine-cholesterol membranes:translational diffusion of a square-planar copper complex in membranes." Biochemistry. 29. 7936-7945 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusumi,A.: "Development of a streak camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells." Biochemistry. 30. 6517-6527 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Subczynski,W.K.: "Effect of alkyl chain unsaturation and cholesterol intercalation on oxygen transport in membranes:a pulse ESR spin labeling study." Biochemistry. 30. 8578-8590 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pasenkiewicz-Gierula,M.: "Influence of phospholipid unsaturation on the cholesterol distribution in the membrane:a review." Biochimie. 73. 1311-1316 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Subczynski,W.K.: "Oxygen transport parameter in rhodopsin as observed by a pulse ESR spin labeling method." Biophys.J.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda,M.: "Two Distinct Light Regulated G-proteins in Octpus Photoreceptors." Biochim.Biophys.Acta. 1052. 204-210 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda,M.: "Cyclic Nucleotides and GTP Analogus Stimulated Light induced Phosphoryration of Octopus Rhodopsin." FEBS Lett.254. 38-40 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deng,H.: "Resonance Raman Studies of the HOOP Mode in Octopus Bathorhodopsin with Deuterium Labeled Retinal Chromophores." Biochemistry. 30. 4495-4502 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada,Y.: "Total Synthesis of a Gene for Octopus Rhodopsin and Its Preliminary Expression." J.Biochem.110. 501-507 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deng,H.: "A Resonance Raman Study of Octopus Bathorhodopsin with Deuterium Labeled Retinal Chromophores." Photochem.Photobiol.54. 1001-1008 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tokunaga: "Structure and Structural Change of the Myosin Head." Advances in Biophysics. 27. 157-167 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinomiya,H.: "Purification and Characterization of the 65-kDa Protein Phosphorylated in Murine Macrophages by Stimulation with Bacterial Lipopolysaccharide." J.Immunol.146. 3617-3625 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta,T.: "The "Lysine Cluster" in the N-terminal Region of Na^+/K^+-ATPase α-subunit is not Involved in ATPase Activity." Biochim.Biophys.Acta. 1059. 157-164 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisabori,T.: "Single site hydrolysis of 2',3'-0-(2,4,6-trinitrophenyl)ATP by F_1-ATPase from a thermophilic bacterium PS3 is accelerated by the chase of excess ATP" J.Biol.Chem.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Muneyuki,E.: "Heterogeneous hydrolysis of substoichiometric ATP by the F_1-ATPase from Escherichia coli" Biochim.Biophys.Acta. 1058. 304-311 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Odaka,M.: "Aromatic Rings of Tyrosine Residue at Adenine Nucleotide Binding Sites of the β Subunit of F1-ATPase Are Not Necessary for ATPase Activity" Biochem.Biophys.Res.Commun.168. 372-378 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 葛西 道生: "生体膜の分子素子・分子機械.生命科学の基礎6.イオン透過とイオノホア." 学会出版センタ-, 25 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷藤 学: "新生化学実験講座6.生体膜と膜輸送.平面膜法によるチャネル電流の計測." 東京化学同人, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 楠見 明弘: "'限界を超える生物顕微鏡'(木下 一彦、宝谷 紘一編)第8章 分子の集まり具合を見る(時間分解蛍光顕微鏡)" 学会出版センタ-, 17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashino,E.: ""Noise Induced Hearing Loss"" Mosby-Year Book, 9 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 曽我部 正博: ""ブレインサイエンスIV"(佐藤編)"機械刺激感受性イオンチャネルのゲ-ティング機構"" 朝倉書店, 22 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murata,M.: ""Biophysics of the Cell Surface"(Glaser,R.,& Gingell,D.,eds.)Membrane Fusion and Lysis by the Amphiphilic Peptides induced by Charge Neutralization:A Model Study of Virus Membrane Fusion Mechanism." Speinger-Verlag,Berlin, 16 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田 基之: "生命科学の基礎6『生体膜の分子素子・分子機械』(池上 明編)光情報の認識処理" 学会出版センタ-, 24 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirata,H.: ""Bioenergetics",(Kim,C.H.and Ozawa,T.,eds.)Reconstitution of H^+-ATPase into Planar Phospholipid Bilayers and its Kinetic Analysis ." Plenum Press,New York, 8 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田 肇: "「新生化学実験講座第6巻生体膜と膜輸送」平面膜へのFoFlーATPaseの組み込みと解析." 東京化学同人, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田 肇: "「膜学実験シリ-ズ」アミノ酸キャリア." 共立出版, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田 肇: "「膜学実験シリ-ズ」平面膜." 共立出版, (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oiki, S.: "Bundles of amphipathic transmembrane alpha-helices as a structural motif for ion-conducting channel proteins : Studies on sodium channels and acetylcholine receptors." Proteins. 8. 226-236 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takumi, T.: "Alteration of channel activities and gating by mutations of slow Isk potassium channel." J. Biol. Chem.266. 22192-22198 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oiki, S.: "A dipolar amino acid substitution induces voltage dependent transitions between two stable conductance states in gramicidin channels." Biophys. J.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nunogaki, K.: "How to separate the H^+ and OH^- permeabilities : Kinetics of intravesicular pH changes after medium pH jump." J. Theor. Biol.144. 415-427 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okihara, K.: "Ca^<2+> and voltage-dependent and calmodulin-antagonist-sensitive anion channel in Chara plasmalemma : A patch clamp study." Plant Cell Physiol.32. 593-601 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ide, T.: "Purification of a K^+-channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system." Biochim. Biophys. Acta. 1067. 213-220 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higuchi, M.: "A simple method for the preparation of an amphiphilic sequential polypeptide." Macromolecules. 23. 361-365 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higuchi, M.: "Channel forming activity of an amphiphilic sequential polypeptide in a cationic bilayer membrane." Bull. Chem. Soc. Jpn.63. 1916-1920 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higuchi, M.: "Interaction between an anionic amphiphilic sequential polypeptide and anionic bilayer membrane." Polymer J.23. 15-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anzai, K.: "Change in intravesicular volume of liposomes by freeze-thawing treatment as studied by the ESR stopped-flow technique." Biochim. Biophys. Acta. 1021. 21-26 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishie, Y.: "Measurement of steady-state Ca-pump current caused by purified Ca-ATPase of sarcoplasmic reticulum incorporated into a planar bilayer lipid membrane." J. Biol. Chem.265. 2488-2491 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anzai, K.: "Formation of ion channels by synthetic basic peptides in planar lipid bilayer membrane." Biochim. Biophys. Acta. 1064. 256-266 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirashima, N.: "Comparative electrophysiological study of reconstituted membrane preparations of the potassium channel of the rabbit skeletal muscle sarcoplasmic reticulum." Biochim. Biophys. Acta. 1067. 235-240 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agawa, Y.: "Interaction with phospholipid bilayers, ion channel formation, and antimicrobial activity of basic amphipathic alpha-helical peptide having various chain length." J. Biol. Chem.266. 20218-20222 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sokabe, M.: "The structure and dynamics of patch clamped membranes : a study by using differential interference light microscopy." J. Cell Biol.111. 599-606 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, K.: "Aminoglycoside blockade of Ca-activated K channels from rat brain synaptosomal membranes incorporated into planar bilayers." J. Membr. Biol.115. 241-251 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sachs, F.: "Stretch activated ion channels and membrane mechanics." Neurosci. Res.12. S1-4 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujitsuka, C.: "Intramembrane particles and responses of sensory axon terminals during reinnervation of the frog muscle spindle." J. Neurocytol.19. 175-186 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sokabe, M.: "Quantitative video microscopy of patch clamped membranes : stress. strain, capacitance, and stretch channel activation." Biophys. J.59. 722-728 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, K.: "Anion channels from rat brain synaptosomal membranes incorporated into planar bilayers." J. Membr. Biol.124. 53-62 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bowman, C. L.: "Mechanotransducing ion channels in astrocytes." Brain Res.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sokabe, M.: "Effects of excision and cytochalasin on the viscoelastic properties of patch clamped membranes in heart and skeletal muscle cells." Biophys. J.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, S.: "Conformation of membrane fusion-active 20-residue peptides with or without lipid bilayers. Implication of alpha-helix formation for membrane fusion." Biochemistry. 29. 6257-6264 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M.: "Membrane fusion induced by mutual interaction of the two charge-reversed amphiphilic peptides at neutral pH." J. Biol. Chem.266. 14353-14358 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M.: "Modification of the N-terminus of membrane fusion-active peptide blocks the fusion activity." Biochem. Biophys. Res. Commun.179. 1050-1055 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M.: "pH-dependent membrane fusion and vesiculation of phospholipid large unilamellar vesicles induced by amphiphilic and cationic peptides." Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pasenkiewicz-Gierula, M.: "Rotational diffusion of a steroid molecule in phosphatidylcholine-cholesterol membranes : fluid phase microimmiscibility in unsaturated-phosphatidylcholine-cholesterol membranes." Biochemistry. 29. 4059-4069 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Subczynski, W. K.: "Microimmiscibility and three-dimensional structure of unsaturated phosphatidylcholine-cholesterol membranes : translational diffusion of a square-planar copper complex in membranes." Biochemistry. 29. 7936-7945 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusumi, A.: "Development of a streak camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells." Biochemistry. 30. 6517-6527 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Subczynski, W. K.: "Effect of alkyl chain unsaturation and cholesterol intercalation on oxygen transport in membranes : a pulse ESR spin labeling study." Biochemistry. 30. 8578-8590 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Subczynski, W. K.: "Oxygen transport parameter in rhodopsin as observed by a pulse ESR spin labeling method." Biophys. J.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: "Two distinct light regulated G-proteins in octopus photoreceptors." Biochim. Biophys. Acta,. 1052. 204-210 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: "Cyclic nucleotides and GTP analogues stimulated light induced phosphorylation of octopus rhodopsin." FEBS Lett.254. 38-40 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deng, H.: "Resonance raman studies of the HOOP mode in octopus bathorhodopsin with deuterium labeled retinal chromophores." Biochemistry. 30. 4495-4502 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, Y.: "Total synthesis of a gene for octopus rhodopsin and its preliminary expression." J. Biochem.110. 501-507 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deng, H.: "A resonance raman study of octopus bathorhodopsin with deuterium labeled retinal chromophores." Photochem. Photobiol.54. 1001-1008 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, M.: "Structure and structural change of the myosin head." Adv. Biophys.27. 157-167 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinomiya, H.: "Purification and characterization of the 65-kDa protein phosphorylated in murine macrophages by stimulation with bacterial lipopolysaccharide." J. Immunol.146. 3617-3625 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, T.: "The "lysine cluster" in the N-terminal region of Na^+/K^+-ATPase alpha-subunit is not involved in ATPase activity." Biochim. Biophys. Acta. 1059. 157-164 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, M.: "Aromatic rings of tyrosine residue at adenine uncleotide binding sites of the bata-subunit of F1-ATPase are not necessary for ATPase activity." Biochem. Biophys. Res. Commun.168. 372-378 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muneyuki, E.: "Heterogeneous hydrolysis of substoichiometric ATP by the F_1-ATPase from Escherichia coli." Biochim. Biophys. Acta. 1058. 304-311 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisabori, T.: "Single site hydrolysis of 2', 3'-0-(2, 4, 6-trinitrophenyl) ATP by F_1-ATPase from a thermophilic bacterium PS3 is accelerated by the chase of excess ATP." J. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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