1990 Fiscal Year Annual Research Report

細胞骨格による細胞接着御機構:分子生物学から臨床医学まで

Research Project

Project/Area Number	02305016
Research Institution	Okazaki National Research Institutes
Principal Investigator	月田承一郎岡崎国立共同研究機構, 生理学研究所, 教授 (50155347)
Co-Investigator(Kenkyū-buntansha)	谷口俊一郎九州大学, 生医研, 助教授 (60117166) 西村孝司東海大学, 医学部・免疫, 助教授 (30143001) 志田寿人山梨医科大学, 生物学部, 助教授 (90004663) 月田早智子岡崎国立共同研究機構, 生理学研究所, 助手 (00188517) 尾張部克志名古屋大学, 理学部, 助手 (90109257)
Keywords	細胞接着 / 細胞骨格 / カドヘリン / ラディキシン / アドヘレンスジャンクション / デスモソ-ム
Research Abstract	本総合研究の初年度は、お互いの研究を発表しあい、細胞間接着の制御やシグナル伝達にいかに細胞骨格が重要な役割を果しているかという点について議論を深めた。特に、この班のメンバ-が中心となって小さな国際シンポジウムを開催するなど、総合研究としてきわめて実りの多い初年度であった。各班員の本年度の業績を以下に要約する。月田(承):細胞間AJ(adherens junction)の裏打ち構造に局在するbarbedーendーcapping蛋白質であるradixinのcDNAを単離し、そのアミノ酸配列を決定した。この蛋白質がband4.1蛋白質のファミリ-に属することが明らかとなった。月田(早):バナジン酸還流組織を抗フォスフォチロシン抗体で蛍光抗体染色することにより、細胞間AJの裏打ち構造がきわめてチロシンキナ-ゼ活性の高い場所であることを見い出した。竹市:カドヘリン結合蛋白質の燐酸化がカドヘリンの接着能を低下させることが明らかになった。尾張部:ヘミデスモソ-ムの単離法を確立し、その接着分子と思われる蛋白質の同定に成功した。谷口:癌転移に抑制的に働くβmアクチンの精製の成功し、in vitroの重合能などを明らかにし、癌転移と細胞骨格の関係を分子レベルで議論することを可能にした。西村:T細胞の接着を阻害する新しいモノクロ-ナル抗体を得ることに成功した。このエピト-プはThy1そのものであることが分かり、この分子の接着における機能が明らかになった。北島:表皮細胞における燐酸化と細胞接着の関係を明らかにした。また、種々の皮膚病における細胞膜裏打ち蛋白質の異常を検索した。志田:デスモソ-ムの接着分子の詳しい分布を調べた。

Research Products
(24 results)

All Other

All Publications (24 results)

[Publications] Tsukita,Sh.: "The undercoat of adherens junctions:A key specialized structure in organogenesis and carcinogenesis." Cell Struct.Funct.15. 7-12 (1990)
[Publications] Nishikawa,S.: "Localization of adherens junction proteins along the possible sliding interface between secretory ameloblasts of the rat incisor." Cell Struct.Funct.15. 245-250 (1990)
[Publications] Sato,N.: "Radixin,a barbedーendーcapping actinーmodulating protein,is concentrated at the cleavage furrow during cytokinesis." J.Cell Biol.
[Publications] Muto,E.: "Doubleーrowed organization of inner dynein arms in Chlamydomonas flagella revealed by tiltーseries thinーsection electron microscopy." J.Cell Sci.
[Publications] Tsukita,Sa.: "Specific protoーoncogenic tyrosine kinases of src family are enriched in cellーtoーcell adherens junctions where the level of tyrosine phosphorylation is elevated." J.Cell Biol.
[Publications] Sasase,A.: "Biochemical analysis of metastasisーrelated A^xactin in B16 Mouse melanoma cells after chemical reversional modulation and of tumor progressionーrelated A^xactin in the ontogeny of human malignant melanoma." Pigment Cell Res.2. 493-501 (1990)
[Publications] OkamotoーInoue,M.: "Alteration in expression of smooth muscle αーactin associated with transformation of rat 3Yl cells." J.Cell Sci.96. 631-637 (1990)
[Publications] Sadano,H.: "Newly identified type of βactin reduces invasiveness of mouse B16ーmelanoma." FEBS LETTERS. 271. 23-27 (1990)
[Publications] Kitajima,Y.: "Partial amino acid sequence of an amyloid fibril protein from nodular primary cutaneous amyloidosis showing homology to λimmunoglobulin light chain of variable subgroup III." J.Invest.Dermatol.95. 301-303 (1990)
[Publications] Hayashi,H.: "Monoclonal antibodies specific to a Ca^<2+>ーbound form of lipocortin I distinguish its Ca^<2+>ーdependent phospholipidーbinding ability from its ability to inhibit phospholipase A_2." Biochem.J.269. 709-715 (1990)
[Publications] Schwarz,M.A.: "Desmosomes and himidesmosomes:Constitutive molecular components." Ann.Rev.Cell Biol.6. 461-491 (1990)
[Publications] Owaribe,K.: "The hemidesmosomal plaque:Identification and characterization of a major constituent protein and experimentally induced endocytosis." Differentiation.
[Publications] Owaribe,K.: "Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells." Exp.Cell Res.
[Publications] Nishimura,K.: "Augmentation of mouse immune functions by dietary restriction:An investigation up to 1 year of age." Ann.Nutr.Metab.34. 76-84 (1990)
[Publications] Nishimura,T.: "Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4^+8^+ tymocytes in vitro." J.Immunol.145. 4012-4017 (1990)
[Publications] Nishimura,T.: "T cell receptorーindependent cellーmediated cytotoxicity by nude mouse lymphokineーactivated killer cells." J.J.Cancer Res.
[Publications] Nakamura,Y.: "MacrophageーT cell interaction is essential for the induction of p75 interleukin 2 (ILー2) receptor and ILー2 responsiveness in human CD 4^+ T cell." J.J.Cancer Res.
[Publications] Shida,H.: "A study of protein Aーgold resolution for immunoelectron microscopy." J.Electron Microsc.Tech.
[Publications] Takeichi,M.: "Cadherins:A molecular family important in selective cellーcell adhesion." Ann.Rev.Biochem.59. 237-252 (1990)
[Publications] Nose,A.: "Localization of specificity determining sites in cadherin adhesion molecules." Cell. 61. 147-155 (1990)
[Publications] Winkel,G.K.: "Activation of protein kinase C triggers premature compaction in fourーcellーstage embryo." Dev.Biol.138. 1-15 (1990)
[Publications] Letourneau,P.C.: "Nerve growth cone migration onto Schwann cells involves the calciumーdependent adhesion molecule,Nーcadherin." Dev.Biol.138. 430-442 (1990)
[Publications] Fujimori,T.: "Ectopic expression of Nーcadherin perturbs histogenesis in Xenopus embryos." Development. 110. 97-104 (1990)
[Publications] Takeichi,M.: "Cadherin cell adhesion receptors as a morphogentic regulator." Science.

1990 Fiscal Year Annual Research Report

細胞骨格による細胞接着御機構:分子生物学から臨床医学まで

Principal Investigator

月田 承一郎 岡崎国立共同研究機構, 生理学研究所, 教授 (50155347)

Research Products

[Publications] Tsukita,Sh.: "The undercoat of adherens junctions:A key specialized structure in organogenesis and carcinogenesis." Cell Struct.Funct.15. 7-12 (1990)

[Publications] Nishikawa,S.: "Localization of adherens junction proteins along the possible sliding interface between secretory ameloblasts of the rat incisor." Cell Struct.Funct.15. 245-250 (1990)

[Publications] Sato,N.: "Radixin,a barbedーendーcapping actinーmodulating protein,is concentrated at the cleavage furrow during cytokinesis." J.Cell Biol.

[Publications] Muto,E.: "Doubleーrowed organization of inner dynein arms in Chlamydomonas flagella revealed by tiltーseries thinーsection electron microscopy." J.Cell Sci.

[Publications] Tsukita,Sa.: "Specific protoーoncogenic tyrosine kinases of src family are enriched in cellーtoーcell adherens junctions where the level of tyrosine phosphorylation is elevated." J.Cell Biol.

[Publications] Sasase,A.: "Biochemical analysis of metastasisーrelated A^xactin in B16 Mouse melanoma cells after chemical reversional modulation and of tumor progressionーrelated A^xactin in the ontogeny of human malignant melanoma." Pigment Cell Res.2. 493-501 (1990)

[Publications] OkamotoーInoue,M.: "Alteration in expression of smooth muscle αーactin associated with transformation of rat 3Yl cells." J.Cell Sci.96. 631-637 (1990)

[Publications] Sadano,H.: "Newly identified type of βactin reduces invasiveness of mouse B16ーmelanoma." FEBS LETTERS. 271. 23-27 (1990)

[Publications] Kitajima,Y.: "Partial amino acid sequence of an amyloid fibril protein from nodular primary cutaneous amyloidosis showing homology to λimmunoglobulin light chain of variable subgroup III." J.Invest.Dermatol.95. 301-303 (1990)

[Publications] Hayashi,H.: "Monoclonal antibodies specific to a Ca^<2+>ーbound form of lipocortin I distinguish its Ca^<2+>ーdependent phospholipidーbinding ability from its ability to inhibit phospholipase A_2." Biochem.J.269. 709-715 (1990)

[Publications] Schwarz,M.A.: "Desmosomes and himidesmosomes:Constitutive molecular components." Ann.Rev.Cell Biol.6. 461-491 (1990)

[Publications] Owaribe,K.: "The hemidesmosomal plaque:Identification and characterization of a major constituent protein and experimentally induced endocytosis." Differentiation.

[Publications] Owaribe,K.: "Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells." Exp.Cell Res.

[Publications] Nishimura,K.: "Augmentation of mouse immune functions by dietary restriction:An investigation up to 1 year of age." Ann.Nutr.Metab.34. 76-84 (1990)

[Publications] Nishimura,T.: "Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4^+8^+ tymocytes in vitro." J.Immunol.145. 4012-4017 (1990)

[Publications] Nishimura,T.: "T cell receptorーindependent cellーmediated cytotoxicity by nude mouse lymphokineーactivated killer cells." J.J.Cancer Res.

[Publications] Nakamura,Y.: "MacrophageーT cell interaction is essential for the induction of p75 interleukin 2 (ILー2) receptor and ILー2 responsiveness in human CD 4^+ T cell." J.J.Cancer Res.

[Publications] Shida,H.: "A study of protein Aーgold resolution for immunoelectron microscopy." J.Electron Microsc.Tech.

[Publications] Takeichi,M.: "Cadherins:A molecular family important in selective cellーcell adhesion." Ann.Rev.Biochem.59. 237-252 (1990)

[Publications] Nose,A.: "Localization of specificity determining sites in cadherin adhesion molecules." Cell. 61. 147-155 (1990)

[Publications] Winkel,G.K.: "Activation of protein kinase C triggers premature compaction in fourーcellーstage embryo." Dev.Biol.138. 1-15 (1990)

[Publications] Letourneau,P.C.: "Nerve growth cone migration onto Schwann cells involves the calciumーdependent adhesion molecule,Nーcadherin." Dev.Biol.138. 430-442 (1990)

[Publications] Fujimori,T.: "Ectopic expression of Nーcadherin perturbs histogenesis in Xenopus embryos." Development. 110. 97-104 (1990)

[Publications] Takeichi,M.: "Cadherin cell adhesion receptors as a morphogentic regulator." Science.

月田承一郎岡崎国立共同研究機構, 生理学研究所, 教授 (50155347)