1990 Fiscal Year Annual Research Report

ホルモンによる遺伝子発現調節および病態に関する研究

Research Project

Project/Area Number	02354011
Research Institution	The University of Tokushima
Principal Investigator	市原明徳島大学, 酵素科学研究センター, 教授 (40035374)
Co-Investigator(Kenkyū-buntansha)	森正敬熊本大学, 医学部, 教授 (40009650) 井村裕夫京都大学, 医学部, 教授 (10025570) 黒木登志夫東京大学, 医科研, 教授 (90006073) 大場義樹金沢大学, 薬学部, 教授 (10012634) 市山新浜松医科大学, 医学部, 教授 (90025601)
Keywords	ホルモン / 遺伝子 / 調節領域 / 転写因子 / クロマチン
Research Abstract	ホルモンは生体制御機構の中で重要な役割を果たしており、その劇的効果は生物学の基礎的研究のみならず、医学の臨床面からも知られている。このようなホルモンの生理作用が古くから明らかにされていたのに較べ、その作用機構についてはようやく最近明らかにされようとしている。ここでは、昨年度に引続き、特に遺伝子発現調節ということに注目し、病態と関連させ、ホルモン作用機構を解明するために研究班を組織し、重点領域研究の申請の準備を行った。 1.<研究状況>___ーーグルココルチコイドとグルカゴンの協調作用を肝臓の糖新生および尿素合成酵素の遺伝子について解析した(市原、森、市山)。作用機序の不明瞭なインスリンについては、解糖系酵素の遺伝子の上流にインスリン応答配列が存在することが明らかとなり、その配列に結合する因子を究明しようとしている(田中)。甲状腺ホルモンおよび活性型ビタミンDによる遺伝子発現調節機構とステロイドホルモンの作用機序との類似性が明らかになりつつある(黒木、藤井、尾形)。抗脂血剤がペルオシゾ-ムの脂肪酸代謝酵素遺伝子群を誘導するのは、その遺伝子の上流に応答配列が存在し、ホルモンと同様の作用機序に基づくことが判明した(橋本)このように遺伝子にシス配列とトランス因子が関与していることが示されてきているが、一方ではDNAの存在様式、即ちクロマチン構造変化がホルモン作用発現に重要であることが示唆され(大場、市原)、新しい手法で研究を進展させたいと考えている。また、ステロイドホルモンをはじめいくつかのホルモンは単に一遺伝子の発現制御というレベルではなく、細胞増殖に制御に働いており、この機構についても解析をしている(松本、杉本)。 2.<研究発表と申請書作成準備>___ーー(1)まず、班員全員による研究発表を京都で行い、討論および今後の方向について活発な意見交換を行った。(2)その後、少人数の班会議を大阪および東京で数回開き、研究方針を立て、重点領域研究の申請書作成を行った。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] H.Yasuda: "The difference in murine cdc2 Kinase activity between cytoplasmic and nuclear fractions during cell cycle" Biochem.Biophys.Res.Commun.172. 371-376 (1990)
[Publications] H.Inoue: "Srrucutures of regulatory regins in the human cytochroma pー450_<scc> (desmolase) gene" Eur.J.Biochem. (1991)
[Publications] T.Murakami: "Promotor and 11ーkilobase upstream enhancer elements responsible for hepatoma cell specific expression of the rat ornithine transcarbamylase gene" Mol.Cell.Biol.10. 1180-1191 (1990)
[Publications] T.Oda: "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine pyruvate aminortansferase of rat liver" J.Biol.Chem. 265. 7513-7519 (1990)
[Publications] E.Ogata: "Control of parathyroid hormone production:Views from a molecular and biological approach" Kidney Int.(1991)
[Publications] T.Usui: "Expression of the human proーopiomelanocortin gene introduced into a rat glial cell line" J.Mol.Endocrinol.4. 169-175 (1990)
[Publications] T.Osumi: "Proliferation of peroxisomes and induction of peroxisomal bーoxidation enzymes in rat hepatoma H4IIEC3 by ciprofibrate" J.Biochem.108. 614-621 (1990)
[Publications] S.Tsuzuki: "Effect of a high protein diet on the gene expression of a trypsinーsensitive cholecystokinin(CCK) releasing peptide(montor peptide) in the pancreas" Eur.J.Biochem.(1991)
[Publications] Chiseko NODA: "Developmental and growth related regulation of expression of serine dehydratase mRNA in liver" Biochem.Biophys.Res.Commun.168. 335-342 (1990)
[Publications] Akira ICHIHARA: "Regulation of protein and amino acid metabolisms in cultured hepatocytes" Nutrition:Protein and Amino Acids. 49-66 (1990)
[Publications] K YAMADA: "Identification and characterization of hepatocyteーspecific regulatory regions of the rat pyruvate kinase Lーtype gene.The synergistic effect of multiple elements" J.Biol.Chem.265. 19885-19891 (1990)
[Publications] K.Yamada: "Tissue specific expression of rat pyruvate kinase L/chloramphenicol acetyltransferase fusion gene in transgenic mice and its regulation by diet and insulin" Biochem.Biophys.Res.Commun.171. 243-249 (1990)
[Publications] C.Noda: "Organization and structure of the 5'flanking region of the rat serine dehydratase gene" J.Biochem.168. 622-628 (1990)
[Publications] A.Tanaka: "Growth stimulation by androgens,glucocorticoids or fibroblast growth factors and the blocking of the stimulated growth by antibody against basic fibroblast growth factor in proteinーfree culture of Shionogi carcinoma 115 cells" J.Steroid Biochem.Mol.Biol.37. 23-29 (1990)
[Publications] T.Watanabe: "Complete nucleotide sequence of human reg gene and its expression in normal and tumoral tissues.The reg protein,pancreatic stone protein and pancreatic thread protein are one and the same product of the gere" J.Biol.Chem.265. 7432-7439 (1990)
[Publications] C.Noda: "Identification of glucocorticoidー and cyclic AMP responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes" Biochem.Biophys.Res.Commun.(1991)

1990 Fiscal Year Annual Research Report

ホルモンによる遺伝子発現調節および病態に関する研究

Principal Investigator

市原 明 徳島大学, 酵素科学研究センター, 教授 (40035374)

Research Products

[Publications] H.Yasuda: "The difference in murine cdc2 Kinase activity between cytoplasmic and nuclear fractions during cell cycle" Biochem.Biophys.Res.Commun.172. 371-376 (1990)

[Publications] H.Inoue: "Srrucutures of regulatory regins in the human cytochroma pー450_<scc> (desmolase) gene" Eur.J.Biochem. (1991)

[Publications] T.Murakami: "Promotor and 11ーkilobase upstream enhancer elements responsible for hepatoma cell specific expression of the rat ornithine transcarbamylase gene" Mol.Cell.Biol.10. 1180-1191 (1990)

[Publications] T.Oda: "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine pyruvate aminortansferase of rat liver" J.Biol.Chem. 265. 7513-7519 (1990)

[Publications] E.Ogata: "Control of parathyroid hormone production:Views from a molecular and biological approach" Kidney Int.(1991)

[Publications] T.Usui: "Expression of the human proーopiomelanocortin gene introduced into a rat glial cell line" J.Mol.Endocrinol.4. 169-175 (1990)

[Publications] T.Osumi: "Proliferation of peroxisomes and induction of peroxisomal bーoxidation enzymes in rat hepatoma H4IIEC3 by ciprofibrate" J.Biochem.108. 614-621 (1990)

[Publications] S.Tsuzuki: "Effect of a high protein diet on the gene expression of a trypsinーsensitive cholecystokinin(CCK) releasing peptide(montor peptide) in the pancreas" Eur.J.Biochem.(1991)

[Publications] Chiseko NODA: "Developmental and growth related regulation of expression of serine dehydratase mRNA in liver" Biochem.Biophys.Res.Commun.168. 335-342 (1990)

[Publications] Akira ICHIHARA: "Regulation of protein and amino acid metabolisms in cultured hepatocytes" Nutrition:Protein and Amino Acids. 49-66 (1990)

[Publications] K YAMADA: "Identification and characterization of hepatocyteーspecific regulatory regions of the rat pyruvate kinase Lーtype gene.The synergistic effect of multiple elements" J.Biol.Chem.265. 19885-19891 (1990)

[Publications] K.Yamada: "Tissue specific expression of rat pyruvate kinase L/chloramphenicol acetyltransferase fusion gene in transgenic mice and its regulation by diet and insulin" Biochem.Biophys.Res.Commun.171. 243-249 (1990)

[Publications] C.Noda: "Organization and structure of the 5'flanking region of the rat serine dehydratase gene" J.Biochem.168. 622-628 (1990)

[Publications] A.Tanaka: "Growth stimulation by androgens,glucocorticoids or fibroblast growth factors and the blocking of the stimulated growth by antibody against basic fibroblast growth factor in proteinーfree culture of Shionogi carcinoma 115 cells" J.Steroid Biochem.Mol.Biol.37. 23-29 (1990)

[Publications] T.Watanabe: "Complete nucleotide sequence of human reg gene and its expression in normal and tumoral tissues.The reg protein,pancreatic stone protein and pancreatic thread protein are one and the same product of the gere" J.Biol.Chem.265. 7432-7439 (1990)

[Publications] C.Noda: "Identification of glucocorticoidー and cyclic AMP responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes" Biochem.Biophys.Res.Commun.(1991)

市原明徳島大学, 酵素科学研究センター, 教授 (40035374)