1991 Fiscal Year Annual Research Report

ペプチドホルモン受容体調節機構のエイジングに関する研究

Research Project

Project/Area Number	02404007
Research Institution	The University of Tokyo
Principal Investigator	川島誠一郎東京大学, 理学部, 教授 (20011554)
Co-Investigator(Kenkyū-buntansha)	朴民根東京大学, 理学部, 助手 (00228694) 岡良隆東京大学, 理学部, 助手 (70143360) 守隆夫東京大学, 理学部, 助教授 (80011659)
Keywords	ゴナドトロピン受容体 / ダウンレギュレ-ション / 受容体遺伝子クロ-ニング / プロラクチン受容体 / ゴナドトロピン放出ホルモン / ペプチド作動性ニュ-ロン / エイジング / GnRH受容体
Research Abstract	ペプチドホルモン受容体調節機耕のエイジングに関する研究において,平成3年度に以下の成果を得た。 (1)マウスでわれわれが発見したFSH受容体のダウンレギュレ-ションが,マウス一般の現象であるか否かを確かめるため数系統で比較したところ,ICR系雄マウスにC57BL系と同様の現象が認められた。 (2)エイジングに併うマウス子宮のプロラクチン受容体量の変化を調べたところ,2か月齢の若いマウスに比べて,7か月齢の成熟マウスではその量が約1/2に減少していることが分った。しかし,生後1か月から同じ系統のマウスの下垂体を異所性移殖して高プロラクチン血症にしておくと,プロラクチン受容体量の減少は生じなしことも明らかになった。 (3)ラットのゴナドトロピン受容体遺伝子はGータンパク質連結受容体ファミリに属し,このファミリの遺伝子で保存性の高い膜通過部位のDNA配列を用いて,マウスの精巣cDNAライブラリからクロ-ニングを行った。しかし,陽性反応を示した3つのクロ-ンを選別し塩基配列を調べた結果,ラットのゴナドトロピン受容体との共通性は見られなかった。この結果は精巣にはゴナドトロピン受容体以外にもGータンパク質と連結して情報を細胞に伝える受容体が存在することを示唆する。現在ラットとヒトの受容体の中でゴナドトロピン受容体に特異的に保存性の高い部位のcDNAを用い,再クロ-ニングを行っている。 (4)ゴナドトロピン放出ホルモン(GnRH)の産生細胞である終神経細胞は,脊椎動物のペプチド作動性ニュ-ロンの神経修飾作用の研究モデルとして適していることが分った。熱帯魚ドワ-フグ-ラミ-の脳標本を用い,終神経GnRH細胞の細胞内記録と細胞分染色により,終神経細胞の自発放電は膜の内因性の性質に起因していること,神経突起の投射域に存在するGnRH受容体を持つ細胞に作用する可能性が分った。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Kengaku,M.: "Expression of choline acetyltransferase activity in a coーculture of spinal cord and skeletal muscle cells is inhibited by myogenic differentiation inhibitors." Developmental Brain Research. 60. 133-136 (1991)
[Publications] Kan,S.: "Effect of vitamin E on the accumulation of fluorescent material in cultured cerbral cortical cells of mice." Experimental Gerontology. 26. 365-374 (1991)
[Publications] Kengaku,M.: "Role of fibronectin in the inhibitory effect of TGFーβ on choline actyltransferase activity in coーcultures of spinal cord neurons and myotubes." Developmental Brain Research. 61. 281-284 (1991)
[Publications] Haruta,K.: "Changes in arginine vasotocin content in the pituitary of the medaka(Oryzias latipes)during osmotic stress." General and Comparative Endocrinology. 83. 327-336 (1991)
[Publications] Yamase,K.: "Circadian changes in arginine vasopressin level in the suprachiasmatic nuclei in the rat." Neuroscience Letters. 130. 255-258 (1991)
[Publications] Mori,T.: "Coincidence of hyperinsulinemis and hyperglycemia after ectopic pituitary grafting in mice." Zoological Science. 8. 339-343 (1991)
[Publications] Mori,T.: "Induction of uterine adenomyosis by pituitary grafting and retardation of its development by bromocriptineーmesilate(CBー154)in BALB/c mice." In Vivo. 5. 107-110 (1991)
[Publications] Mori,T.: "Animal model of uterine adenomyosis: Is prolactin a potent inducer of adenomyosis in mice?" American Journal of Obstetrics and Gynecology. 165. 232-234 (1991)
[Publications] Singtipop,T.: "Developement of uterine adenomyosis after treatment with dopamine antagonists in mice." Life Science. 49. 201-206 (1991)
[Publications] Oka,Y.: "Ultrastructure of the ganglion cells of the terminal nerve in the dwarf gourami(Colisa lalia)." Journal of Compafative Neurology. 304. 161-171 (1991)
[Publications] IChikawa,M., (Ed.by A.Yokoyama): "Gonadotropinーreleasing hormone neurons in the olfactory system." “Brain control of the reproductive systems,Springer Verlag.(1992)
[Publications] Nagai,T.: "The glosspharyngeal nerve of the Axolotle with carbocyanine dye(DiI)." Neuroscinece Letters.131. 125-128 (1991)
[Publications] Oka,Y., (Ed.by K.Kubota): "The terminal nerve GnRH system of fishーa model system for the study of peptidergic neuromodulation?" Proceedings of the International Symposium on Behavior and Neuroethology,Kyoto Univ.Press. (1991)
[Publications] Amano,M.: "Immunocytochemical demonstration of aslmon GnRH and chicken GnRHーII in the brain of masu salmon,Oncorhynchus masou." Journal of Comparative Neurology. 314. 587-597 (1991)
[Publications] Tanaka,S.: "Differential appearance of the subunits of glycoprotein hormones(LH,FSH and TSH)in the pituitary of bullfrog(Rana catesbeiana)larvae during metamorphosis." General and Comparative Endoソrinology. 84. 318-327 (1991)
[Publications] Miller,M.: "Nuclear pore complex:structure,function,and regulation." Physiological Reviews. 71. 909-949 (1991)
[Publications] 窪川かおる,守隆夫編集: "ホルモン実験ハンドブック I.飼育と手技" 学会出版センタ-, 218 (1991)
[Publications] 窪川かおる,守隆夫編集: "ホルモン実験ハンドブック III.実験のモデル" 学会出版センタ-, 181 (1991)

1991 Fiscal Year Annual Research Report

ペプチドホルモン受容体調節機構のエイジングに関する研究

Principal Investigator

川島 誠一郎 東京大学, 理学部, 教授 (20011554)

Research Products

[Publications] Kengaku,M.: "Expression of choline acetyltransferase activity in a coーculture of spinal cord and skeletal muscle cells is inhibited by myogenic differentiation inhibitors." Developmental Brain Research. 60. 133-136 (1991)

[Publications] Kan,S.: "Effect of vitamin E on the accumulation of fluorescent material in cultured cerbral cortical cells of mice." Experimental Gerontology. 26. 365-374 (1991)

[Publications] Kengaku,M.: "Role of fibronectin in the inhibitory effect of TGFーβ on choline actyltransferase activity in coーcultures of spinal cord neurons and myotubes." Developmental Brain Research. 61. 281-284 (1991)

[Publications] Haruta,K.: "Changes in arginine vasotocin content in the pituitary of the medaka(Oryzias latipes)during osmotic stress." General and Comparative Endocrinology. 83. 327-336 (1991)

[Publications] Yamase,K.: "Circadian changes in arginine vasopressin level in the suprachiasmatic nuclei in the rat." Neuroscience Letters. 130. 255-258 (1991)

[Publications] Mori,T.: "Coincidence of hyperinsulinemis and hyperglycemia after ectopic pituitary grafting in mice." Zoological Science. 8. 339-343 (1991)

[Publications] Mori,T.: "Induction of uterine adenomyosis by pituitary grafting and retardation of its development by bromocriptineーmesilate(CBー154)in BALB/c mice." In Vivo. 5. 107-110 (1991)

[Publications] Mori,T.: "Animal model of uterine adenomyosis: Is prolactin a potent inducer of adenomyosis in mice?" American Journal of Obstetrics and Gynecology. 165. 232-234 (1991)

[Publications] Singtipop,T.: "Developement of uterine adenomyosis after treatment with dopamine antagonists in mice." Life Science. 49. 201-206 (1991)

[Publications] Oka,Y.: "Ultrastructure of the ganglion cells of the terminal nerve in the dwarf gourami(Colisa lalia)." Journal of Compafative Neurology. 304. 161-171 (1991)

[Publications] IChikawa,M., (Ed.by A.Yokoyama): "Gonadotropinーreleasing hormone neurons in the olfactory system." “Brain control of the reproductive systems,Springer Verlag.(1992)

[Publications] Nagai,T.: "The glosspharyngeal nerve of the Axolotle with carbocyanine dye(DiI)." Neuroscinece Letters.131. 125-128 (1991)

[Publications] Oka,Y., (Ed.by K.Kubota): "The terminal nerve GnRH system of fishーa model system for the study of peptidergic neuromodulation?" Proceedings of the International Symposium on Behavior and Neuroethology,Kyoto Univ.Press. (1991)

[Publications] Amano,M.: "Immunocytochemical demonstration of aslmon GnRH and chicken GnRHーII in the brain of masu salmon,Oncorhynchus masou." Journal of Comparative Neurology. 314. 587-597 (1991)

[Publications] Tanaka,S.: "Differential appearance of the subunits of glycoprotein hormones(LH,FSH and TSH)in the pituitary of bullfrog(Rana catesbeiana)larvae during metamorphosis." General and Comparative Endoソrinology. 84. 318-327 (1991)

[Publications] Miller,M.: "Nuclear pore complex:structure,function,and regulation." Physiological Reviews. 71. 909-949 (1991)

[Publications] 窪川 かおる,守 隆夫 編集: "ホルモン実験ハンドブック I.飼育と手技" 学会出版センタ-, 218 (1991)

[Publications] 窪川 かおる,守 隆夫 編集: "ホルモン実験ハンドブック III.実験のモデル" 学会出版センタ-, 181 (1991)

川島誠一郎東京大学, 理学部, 教授 (20011554)

[Publications] 窪川かおる,守隆夫編集: "ホルモン実験ハンドブック I.飼育と手技" 学会出版センタ-, 218 (1991)

[Publications] 窪川かおる,守隆夫編集: "ホルモン実験ハンドブック III.実験のモデル" 学会出版センタ-, 181 (1991)