1993 Fiscal Year Final Research Report Summary
Biologically active substances produced by Bacteroides gingivalis and their periodontopathogenicity
| Project/Area Number |
02404078
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAKAMURA Ryo The University of Tokushima, School of Dentistry, Professor, 歯学部, 教授 (30034169)
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| Co-Investigator(Kenkyū-buntansha) |
MORIOKA Masami The University of Tokushima, School of Dentistry Research Associate, 歯学部, 助手 (90243708)
HAYASHI Hiroyuki The University of Tokushima, School of Dentistry Research Associate, 歯学部, 助手 (80243707)
HINODE Daisuke The University of Tokushima, School of Dentistry Assistant Professor, 歯学部・附属病院, 講師 (70189801)
NAGATA Atsushi The University of Tokushima, School of Dentistry Assistant Professor, 歯学部・附属病院, 講師 (70228021)
SATO Makoto The University of Tokushima, School of Dentistry Associate Professor, 歯学部, 助教授 (10126229)
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| Project Period (FY) |
1990 – 1993
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| Keywords | Bacteroides gingivalis / Perodontopathogenicity / Hemagglutinin / Trypsin-like enzyme / Band 3 protein / Tissue-cell agglutinin / Cytotoxicity / Vesicle |
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| Research Abstract |
Bacteroides gingivalis produces several types of biologically active substances. The purpose of this investigation is to determine the properties of these substances such as proteases, hemagglutinin and tissue-cell agglutinating factor etc. in association with periodontopathogenicity and cytotoxicity.
Hemagglutinin (HA), which is considered to participate in the bacterial adherence to host tissues, was isolated and purified from the culture supernatant. The receptor protein of the erythrocyte for this HA was clarified to be band 3 protein and the arginine residue of the peptide was concerned in the binding. This HA possessed both the trypsin-like and the tissue-cell aggregating activities, suggesting that hemagglutination may occur as a result of enzyme-substrate reaction between the trypsin-like enzyme and the band 3 protein.
The trypsin-like enzyme produced by B.gingivalis showed the ability of hydrolyzing type I collagen, IgG, IgA and some other naturally occurring proteins. This enzyme also had strong cytotoxicity on human gingival fibroblasts. These facts indicate that the enzyme exhibits pathogenicity not only by destroying periodontal tissue directly but also by decreasing host immune defense ability.
All these biologically active substances are concentrated in the secreted outer membrane vesicles of B.gingivalis and all of them behaved in the same way on several protease inhibitors. These results suggest that the true feature of the pathogenic factor of B.gingivalis could be the trypsin-like protease which associates with the outer membrane vesicles.
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[Publications] Hinode, D., Nagata, A., Hayashi, H., Shimada, J., Sato, M.and Nakamura, R.: "Degradative specificity of three proteases from Porphyromonas gingivalis on mammalian protein." Dent.in Japan. 28. 121-124 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Morioka, M., Kido, R., Hinode, D., Nagata, A., Sato, M.and Nakamura, R.: "Adhesion of Porphyromonas gingivalis to human gingival fibroblast and collagen." J.Dent.Health. 42. 23-34 (1992)
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[Publications] Morioka, M., Kido, R., Nagata, A., Hinode, D., Ichimiya, S., Sato, M., Nakamura, R.: "Study on the cytotoxic activity of Porphyromonas gingivalis for the cultured human fibroblast" J.Dent.Health. 42. 184-190 (1992)
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[Publications] Hinode, D., Nagata, A., Ichimiya, S., Hayashi, H., Morioka, M.and Nakamura, R.: "Generation of plasma kinin by three types of proteases isolated from Porphyromonas gingivalis 381." Arch.Oral Biol.37. 859-861 (1992)
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[Publications] Morioka, M., Hinode, D., Nagata, A., Hayashi, H., Ichimiya, S., Ueda, M., Kido, R.and Nakamura, R.: "Cytotoxicity of Porphyromonas gingivalis toward cultured human gingival fibroblasts." Oral Microbiol. Immunol.8. 203-207 (1993)
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[Publications] Hayashi, H., Morioka, M., Ichimiya, S., Yamato, K., Hinode, H., Nagata, A.and Nakamura, R.: "Participation of an arginyl residue of insulin chain B in the inhibition of hemagglutination by Porphyromonas gingivalis." Oral Microbiol Immunol.8. 386-389 (1993)
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[Publications] Morioka, M., Ueda, M., Yamato, K., Hayashi, H., Hinode, D.Nagata, A.and Nakamura, R.: "Cell-agglutinating activity of Porphyromonas gingivalis for cultured human fibroblasts." J.Dent.Health.44. 116-121 (1994)
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[Publications] Nakamura, R., Hinode, D., Terai, H.and Morioka, M.: Extracellular enzymes of Porphyromonas (Bacteroides) gingivalis in relation to periodontal destruction. in : Periodontal Disease : Pathogens & Host Immune Responses (Edited by Hamada, S., Holt, S.C.and McGhee, J.R.). Quintessence Publishing, Tokyo, 129-141 (1991)
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