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1993 Fiscal Year Final Research Report Summary

Pulsed Electric Dichroism Spectrophotometer with Multichannel Detection System and Higher-Order Structure of Natural and Synthetic DNA

Research Project

Project/Area Number 02405008
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YAMAOKA Kiwamu  Hiroshima U., Fac.Sci., Professor, 理学部, 教授 (40033815)

Co-Investigator(Kenkyū-buntansha) FUKUDOME Kiyohiro  Hiroshima U., Fac.Sci., Assistant, 理学部, 助手 (50183588)
OCHIAI Hiroshi  Hiroshima U., Fac.Sci., Asst.Prof., 理学部, 助教授 (50033840)
YAMAMOTO Osamu  Hiroshima U., Fac.Sci., Professor, 理学部, 教授 (80034627)
Project Period (FY) 1990 – 1993
KeywordsPulsed Electric Dichroism / Double-Stranded Helical Structure / Synthetic Polydeoxyribonucleotides / Plasmid DNA / Ultraviolet Linear Dichroism Spectrum / Electric Field Orientation Mechanism / Electric Fluorescence Dichroism / DNA-Dye Complexes
Research Abstract

1. Design and Construction of the Apparatus
A novel high-speed pulsed electric dichroism spectrophotometer was designed and completed in this laboratory with the aid of the title grant as cited above, together with the computer softwares for connecting the high-voltage electric pulse delivery system and the optical detecting system with the spectrophotometer, which is abbreviated as the IMPAD-SPED (instant multichannel photodiode array detecting single-pulsed electric dichroism) spectrophotometer. This commercial spectrophtometer was, however, found to have an inherent drawback that is associated with the ultraviolet stray light below 300 nm. Attempts were made to remove this difficulty as well as a problem of the secondary emission from the image intensifier of the signal detector in collaboration with the manufacturer, but ended unsuccessfully. Thus, the quantitative measurement of electric linear dichroism spectra was limited to samples, such as DNA-dye complexes, which show strong a … More bsorption spectra in the visible and near UV (300-700 nm) regions.
2. Electric Linear Dichroism (ELD) Spectra Measurements
Ethidium bromide (EtBr) and the mono- and diacetylated derivatives, bound to sonicated and fractionated low molecular weight calf thymus DNA as well as large plasmid DNA,were studied with the IMPAD-SPED instrument. The ELD spectra showed that the plane of the bound dye is inclined as well as tilted with respect to the double-stranded DNA helical axis. Poly (A) ・Poly (U) -EtBr complexes are now being worked out for publication. A detailed analytical method of ELD spectra was developed for structural determination. With the conventional techniques, the B**Z transition of Poly (dG-dC) duplex and the triple helix formation of Poly (A) ・2Poly (I) were studied.
3. Presentation of Research Achievements
In the fiscal 1994 year, totally 7 papers were presented at scientific meetings of Polymer Society Japan, Colloid and Surface Chemistry Symposium, Biophysics Society of Japan, and Nucleic Acids Symposium. During the same period, totally 9 papers were published as shown in the reverse page. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] M.Tanigawa et al.: "Molecular Weight Distribution and Intrinsic Viscosity of Sonicated and Successively Fractionated Double-Stranded Deoxyribonucleic Acid and Polyribonucleotides." Polymer Journal. 26. 291-302 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Ojima et al.: "Determination of Molecular Weight Distribution and Spectroscopic and Viscometric properties of Sonicated Triple-Stranded Polyribonucleotides." Polymer Journal. 26. 303-314 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaoka et al.: "Reversing-Pulse Electric Birefringence of Disklike Suspension in the Low Electric Field Region: An Extension of the Ion-Fluctuatin Model." Journal of Chemical Physics. 101. 1625-1631 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaoka et al.: "Reversing-Pulse Electric Birefringence of Montmoril-lonite Particles Suspended in Aqueous Media." Chemistry Letters. 563-566 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaoka and N.Takata: "A Novel High-Speed Pulsed Electric Dichroism Spectro-photometer with Multichannel Photodiode Detection System. Construction of Apparatus and Application to DNA-Dye Complex Solution." Chemistry Letters. 1503-1506 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaoka et al.: "Electro-Optics in Dispersed Systems. II. Reversing-Pulse Electric Birefringence of Polystyrene Latex Particles in Aqueous Media." Journal of Colloid and Interface Science. 168. 349-355 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tanigawa and K.Yamaoka: "Electro-Optical and Hydrodynamic Properties of Synthetic Polyribonucleotides in Solutions As Studied by Electric Birefringence." Bulletin of Chemical Society of Japan. 68. 481-492 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukudome et al.: "Sonication,Fractionation,and Molecular Weight Distribution of Alternating Polydeoxyribonucleotides: Poly(dG-dC)・Poly(dG-dC) and Poly(dA-dT)・Poly(dA-dT)." Polymer Journal. 27. 101-110 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Ojima et al.: "Structural Determination of Nucleic Acids in Solution by Electric Linear Dichroism Method." Journal of Science of the Hiroshima University,Series A,. 59. 119-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukudome, K.Iwasaki, S.Matsumoto, and K.Yamaoka: "Undesirable Contamination of DNA Electrophoresed Through Polyacrylamide and Agarose Gels As Revealed by Reversing-Pulse Electric Birefringence Signals." Biopolymers. 31. 1455-1458 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka: "Electric Field Orientation of Double-Stranded Nucleic Acids in Aqueous Solutions As Studied by Electric Linear Dichroism, in Colloid and Molecular Electro-Optics 1991." IOP Publishing Ltd, Bristol. 233-238 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka, M.Kimura, and M.Okada: "Electric Field Orientation of Ionizable Polymers with Three Different Electric Dipole Moments. I.New Orientation Function and the Steady-State Electric Birefringence of Poly (L-alpha-glutamic acid) in Methanolic and Aqueous Solutions." Bull.Chem.Soc.Jpn.65. 129-139 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka, K.Fukudome, and K.Matsuda: "Electric Field Orientation of Nucleic Acids in Aqueous Solutions. 3. Non-Kerr-Law Behavior of High Molecular Weight DNA at Weak Fields As Revealed by Electric Birefringence and Electric Dichroism." J.Phys.Chem.96. 7131-7136 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tanigawa, N.Mukaiyama, S.Shimokubo, K.Wakabayashi, Y.Fujita, K.Fukudome, and K.Yamaoka: "Molecular Weight Distribution and Intrinsic Viscosity of Sonicated and Successively Fractionated Double-Stranded Deoxyriboucleic Acid and Polyribonucleotides." Polymer J.26. 291-302 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Ojima, K.Fukudome, and K.Yamaoka: "Determination of Molecular Weight Distribution and Spectroscopic and Viscometric Properties of Sonicated Trple-Stranded Polyribonucleotides." Polymer J.26. 303-314 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka, M.Tanigawa, and R.Sasai: "Reversing-Pulse Electric Birefringence of Disklike Suspension in the Low Electric Field Region : An Extension of the Ion-Fluctuation Model." J.Chem.Phys.101. 1625-1631 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka, R.Sasai, and N.Ikuta: "Reversing-Pulse Electric Birefringence of Montmorillonite Partocles Suspended in Aqueous Media." Chem.Lett.563-566 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka and N.Takata: "A Novel High-Speed Pulsed Electric Dichroism Spectrophotometer with Multichannel Photodiode Detection System. Construction of Apparatus and Application to DNA-Dye Complex Solution" Chem.Lett.1503-1506 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaoka, K.Fukudome, S.Matsumoto, and Y.Hino: "Electro-Optics in Dispersed Systems. II.Reversing-Pulse Electric Birefringence of Polystirene Latex Particles in Aqueous Media" J.Colloid and Interface, Sci.168. 349-355 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tanigawa and K.Yamaoka: "Electro-Optical and Hydrodynamic Properties of Synthetic Polyribonucleotides in Solutions As Studied by Electric Birefringence." Bull.Chem.Soc.Jpn.68. 481-492 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Fukudome, Y.Kumamoto, and K.Yamaoka: "Sonication, Fractionation, and Molecular Weight Distribution of Alternating Polydeoxyribonucleotides : Poly (dG-dC) ・Poly (dG-dC) and Poly (dA-dT) ・Poly (dA-dT)." Polymer J.27. 101-110 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Ojima, K.Fukudome, and K.Yamaoka: "Structural Determination of Nucleic Acids in Solution by Electric Linear Dichroism Method." J.Sci.Hiroshima Univ., Ser.A. 59. 119-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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