1991 Fiscal Year Final Research Report Summary
Design and Synthesis of DNA-Cleaving Molecules
| Project/Area Number |
02453023
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
有機化学一般
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| Research Institution | Kyoto University |
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Principal Investigator |
SAITO Isao Kyoto University, Faculty of Engineering, Professor, 工学部, 教授 (20026082)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Hiroshi Kyoto Univ., Faculty of Engineering. Research Associate, 工学部, 助手 (50183843)
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| Project Period (FY) |
1990 – 1991
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| Keywords | DNA Cleavage / Ene-diyne Antitumor Antibiotics / Artificial Restriction Enzym / Photo-DNA-Cleavage / Hydroxyl Radical Cenerator |
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| Research Abstract |
There has been great interest in designing reagents that bind to DNA duplex selectively at desired sequences. Such molecules equipped with a chemical or photochemical tag capable of modifying or cleaving DNA at the site of binding should have great prospects as "synthetic artificial restriction enzymes" and not least as gene-selective drugs. We have focused on the use of natural DNA-binders such as antitumor antibiotics, DNA-binding proteins or sequence-defined oligonucleotides in combination with photoactivated DNA-cleaving molecules. We have succeeded in designing a new sequence specific "photochemical DNA cleaver" that binds specifically to-5'-GG-3'sequence. This molecule named as "photo-Fenton-reagent" is widely used as a convenient source of hydroxyl radical. We also succeeded in designing a different type of DNA-cleaving molecules based on the conceit obtained from the work on the action mechanism of ene-diyne type antitumor antibiotics such as neocarzinostatin or esperamicin.
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