1991 Fiscal Year Final Research Report Summary
Membrane protein transfer from intact cell to liposome
| Project/Area Number |
02453099
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子物性・高分子材料（含機械材料）
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SUNAMOTO Junzo Kyoto University, Polymer Chemistry, Professor, 工学部, 教授 (80037811)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Toshinori Kyoto University, Polymer Chemistry, Assistant Professor, 工学部, 助手 (00162454)
AKIYOSHI Kazunari Kyoto University, Polymer Chemistry, Assistant Professor, 工学部, 助手 (90201285)
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| Project Period (FY) |
1990 – 1991
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| Keywords | membrane protein / liposome / transfer / liposomal vaccine / cancer cell / boundary lipid / erythrocyte / platelet |
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| Research Abstract |
Perfect extraction of intrinsic membrane proteins from intact cells and reconstitution of them without any denaturation and deactivation are basic requirement in investigation of the function of membrane proteins. For this purpose, artificial cell liposome seem to be a desirable and promising tool in both basic investigation and application. In order to improve the reconstitution efficiency of membrane proteins into liposomes, we have developed a novel artificial boundary lipid, 1,2-dimyristoylamido-1,2-deoxy- phosphatidylcholine (DDPC), of which alkyl chains are linked by an amide bond. When DDPC-containing liposomes were coincubated with intact cells such as erythrocyte, platelet and tumor cell, several membrane proteins were able to be effectively transferred from the intact cell to the liposome. Using this technique, furthermore, we succeeded in development of liposomal vaccine against BALB RVD leukemia cell for tumor surface antigenic proteins (TSAP) transferred from the tumor cells to liposomes.
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