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1992 Fiscal Year Final Research Report Summary

Regeneration of the cerebellofugal projections and reconstruction of the projections by grafting of embryonic brain structures.

Research Project

Project/Area Number 02454121
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KAWAGUCHI Saburo  Dept. Integ. Brain Sci., Fac. Med., Kyoto Univ., Associate Prof., 医学部, 助教授 (70024635)

Project Period (FY) 1990 – 1992
KeywordsSpinal cord injury / Spinal cord grafting / Cerebellar grafting / Regeneration of CNS pathway / Reconstruction of CNS circuitries / Functional recovery / 神経回路の再構築
Research Abstract

In contrast to the current concept that regeneration of the mammalian CNS axons is very limited and has no functional significance, the present studies revealed that marked, functionally significant regeneration of CNS axons occurred after complete transection of the spinal cord in the rat and that either the cerebellum or the spinal cord segments in neonatal rats were replaceable.
1)Spinal Cord Regeneration The spinal cord of neonatal rats was transected completely at a level of the lower thoracic segments. One to two months after transection, neural connections beyond the transection were examined by retrograde labeling with fluorescence dyes injected into the lumber enlargement or the upper cervical segments. In the animals operated on before 18 days post partum, the descending and ascending pathways traversed the site of transection were indistinguishable from normal. Locomotor function of these animals was also indistinguishable from normal.
2)Replacement of the Cerebellum The whole … More cerebellum or hemicerebellum of neonatal rats was replaced with embryonic homologous structures. Homotopically grown grafts became as large as the normal cerebellum exhibiting normal cytoarchitecture and formed input and output connections indistingusihable from normal. The grafts, thus, appeared to have established neural integrity with the host brain in a manner very similar to normal ontogenesis.
3)Replacement of the Spinal Cord The lower thoracic segments of the neonatal rats were replaced with embryonic homologous structures. The grafted embryonic spinal cord segments grew and united with the host spinal cord at both the rostral and caudal stumps to form a seamless spinal cord. Anterograde and retrograde labeling with tracer demonstrated that neural connections hardly distinguishable from normal were formed between the lumber enlargement and upper brain structures traversing the graft. These animals walked, ran, and climbed with coordination of fore- and hindlimbs in a manner strikingly similar to normal. Less

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Okuda,B.,Kawaguchi,S.,Yamamoto,T.,Samejima,A.: "Changes in the cerebello-cerebral response in the parietal cortex following ablation of the motor cortex in the cat:early occurrence and persistence." Neurosci.Res.8. 1-11 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川口 三郎: "哺乳動物における中枢神経伝導路の再生." 神経科学レビュー. 4. 100-132 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi,S.,Murata,M.,Kurimoto,Y.: "Ontogenesis of the cerebellofugal projection in the rat." Dev.Brain Res.61. 285-289 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi,S.: "Regeneration and reconstruction of neural connections in the cerebellum." Neurosci.Res.Suppl.17. S14 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi,S.,Murata,M.,Iwashita,Y.: "Spinal cord regeneration after complete transection in neonatal rats." Neurosci.Res.Suppl.17. S185 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwashita,Kawaguchi,S.,Murata,M.: "Replacement of the spinal cord in neonatal rats by embryonic homotopic structures." Neurosci.Res.Suppl.17. S185 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi,S.: "Reconstruction of mammalian central neural circuity.In T.Ono,L.R.Squire,M.E.Raichle,D.I.Perrett,M.Fukuda(Eds),″Brain Mechanisms of Perception and Memory:From Neuron to Behavior″" Oxford University Press,

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawaguchi, S., Murata, M., Kurimoto, Y.: "Ontogenesis of the cerebellofugal projection in the rat." Neurosci. Res.9 Suppl.11. S34 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda, B., Kawaguchi, S., Yamamoto, T., Samejima, A.: "Changes in the cerebello-cerebral response in the parietal cortex following ablation of the motor cortex in the cat: early occurrence and persistence." Neurosci. Res.8. 1-11 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawaguchi, S., Murata, M., Kurimoto, Y.: "Ontogenesis of the cerebellofugal projection in the rat." Dev. Brain Res.61. 285-289 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawaguchi, S., Murata, M., Kurimoto, Y.: "Homotopical grafting of embryonic hemicerebellum in cerebellectomized neonatal rats." Neurosci. Res.Suppl.14. S38 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurimoto, Y., Kawaguchi, S., Murata, M.: "Cerebellotectal projection in the rat: anterograde and retrograde WGA-HRP study." Neurosci. Res.Suppl.14. S76 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurimoto, Y., Kawaguchi, S., Murata, M.: "Plasticity of the cerebellotectal projection after neonatal hemicerebellectomy in the rat." Jpn. J. Physiol.41 Suppl.S264 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murata, M., Kawaguchi, S., Kurimoto, Y.: "Afferent into grafted cerebellum in rat revealed by anterograde PHA-L method." Neurosci. Res.Suppl.16. S92 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurimoto, Y., Kawaguchi, S., Murata, M.: "Reorganization of the cerebellotectal projection after" Jpn. J. Physiol.42 Suppl.S224 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawaguchi, S.: "Regeneration and reconstruction of neural connections in the cerebellum." Neurosci. Res.Suppl.17. S14 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawaguchi, S., Murata, M., Iwashita, Y.: "Spinal cord regeneration after complete transection in neonatal rats." Neurosci. Res.Suppl.17. S185 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwashita, Y., Kawaguchi, S., Murata, M.: "Replacement of the spinal cord in neonatal rats by embryonic homotopic structures." Neurosci. Res.Suppl.17. S185 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawaguchi, S.: "Reconstruction of mammalian central neural circuitry." In T. Ono, L. R. Squire, M. E. Raichle, D. I. Perrett, M. Fukuda (Eds), "Brain Mechanisms of Perception and Memory: From Neuron to Behavior" Oxford University Press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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