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1991 Fiscal Year Final Research Report Summary

Mechanisms underlying generation and activation of class II MHC-restricted B lymphocytes and their function

Research Project

Project/Area Number 02454189
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionOsaka University

Principal Investigator

HAMAOKA Toshiyuki  Osaka University Medical School, Professor, 医学部, 教授 (60028529)

Co-Investigator(Kenkyū-buntansha) ONO Shiro  Osaka University Medical School, Lecturer, 医学部, 講師 (80127208)
Project Period (FY) 1990 – 1991
Keywordsclass II MHC / B-lymphocyte / MHC-restriction / Surface Immunoglobulin / B-lymphocyte Repertoire / genetic control / autoantibody / signal Transduction
Research Abstract

1. I-A molecules serve as restriction elements of the class 11 major histocompatibility complex (MHC) -restricted B-B cell interaction involved in the polyclonal B cell differentiation, but I-E molecules do not exhibit such activity. Moreover, cross-linking by anti-I-E mAb of I-E molecules on B cells induces increases in intracellular CAMP levels, whereas such increases are not elicited by cross-linking of I-A molecules, indicating disparate function of I-A and I-E molecules expressed on B cells.
2. The B lymphoma cells expressing relevant I-A molecules function as auxiliary cells in the class II MHC-restricted B cell activation, While neither I-A-positive macrophage lines nor I-A-transfected fibroblasts collaborate with class II NMC-restricted B cells. Thus, B-cell unique signals play a critical role in the B-cell activation.
3. It is shown that there exist at least two distinct mechanisms in the T-independent B cell activation pathway. Anti-trinitrophenyl (TNP) antibody responses induced by LPS require class 11 MHC-restricted B-B cell inter-action but not surface Ig-mediated signaling, whereas those evoked by TNP-LPS con ugates are dependent on signaling through surface Ig but not by class 11 MHC molecules. This implies that signal transduction through surface Ig overcomes the requirement for class 11 MHC-mediated signaling.
4. In the anti-BrMRBC (bromelain-treated mouse red blood cell) autoantibody responses induced by LPS, genotypically low-responder B cells are converted to highresponder phenotype if the low-responder B cells mature under the conditions in which they are able to acquire the restriction specificity for high-responder type of class 11 MHC molecules.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Takahama et al.: "Involvement of I-A-restricted B-B cell Interaction in the rolyclonal B cell differentiation induced by lipopolysaccharide" Advances in Experimental Medicine and Biology In “Endotoxin" Eds.by M.Nakano,A.Nowotony,H.Friedman and T.Klein,Plenum Publish.Corp.256. 427-443 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Takahama et al.: "B-B cell interaction involved in poly clonal B cell activation is restricted by I-A but not by I-Emolecules" International Immunol.2. 63-71 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Miyake et al.: "Monoclonal antibodies to Pgp-1/CD44 block lymphohemopoiesis in long-term bone marrow cultures" J.Exp.Med.171. 477-488 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yoshimoto et al.: "IL-5 up-regulates but IL-4 down-regulates IL-2R expression on a cloned B lymphoma line" J.Immunol.144. 183-190 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nakanishi et al.: "Lymphokine-regulated differential expression of mRNA for p75KDa-IL-2R and p55KDa-IL-2R in a cloned B lymphoma line(BLC1-CL-3 cells)" J.Immunol.145. 1423-1429 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Takai et al.: "Identification of IL-7-dependent bone marrow-derived Thy-1^- B220^- lymphoid cell clones that rearrange and express both immunoglobulin and T cell receptor genes" J.Immunol.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Takahama et al: "Involvement of I-A-restricted B-B cell interaction in the polyclonal B cell differentiation induced by lipopolysaccharide" Advances in Experimental Medicine and Biology. 256. 427-443 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Takahama et al.: "B-B cell interaction involved in polyclonal B cell activation is restricted by I-A but not by I-E molecules" International Immunol.2. 63-71 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Miyake et al.: "Monoclonal antibodies to Pgp-1/CD44 block lympho-homopoiesis in long-term bone marrow cultures" J. Exp. Med.171. 477-488 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yoshimoto et al.: "IL-5 up-regulates but IL-4 down-regulates IL-2R expression on a cloned B lymphoma line" J. Immunol.144. 183-190 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nakanishi et al.: "Lymphokine-regulated differential expression of mRNA for p75KDa-IL-2R and p55KDa-IL-2R in a Cloned B lymphoma line (BLC_1-CL-3 cells)" J. Immunol.145. 1423-1429 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Takai et al.: "Identification of IL-7-dependent bone marrow-derived Thy-1^- B220^- lymphoid cell clones that rearrange and express both immunoglobulin" tor genes.J. Immunol.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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