1991 Fiscal Year Final Research Report Summary
Studies on the Signal Transduction in T Lymphocytes via Antigen and Lymphokine Receptors.
Project/Area Number |
02454194
|
Research Category |
Grant-in-Aid for General Scientific Research (B)
|
Allocation Type | Single-year Grants |
Research Field |
Immunology
|
Research Institution | Jichi Medical School |
Principal Investigator |
MINATO Nagahiro Jichi Medical School, Dept. of Medicine, Associate Prof., 医学部, 助教授 (40137716)
|
Co-Investigator(Kenkyū-buntansha) |
HATTORI Masakazu Hokkaido Univ., Dept. of Veterinary Medicine, Assistant Prof., 獣医学部, 助手 (40211479)
|
Project Period (FY) |
1990 – 1991
|
Keywords | Interleukin 2 / IL 2 receptor / Transgenic mice / CD45 / Phosphatidylinositol / Signal transduction / DNA-binding protein |
Research Abstract |
By the analysis of human IL 2R(alpha)-transgenic mice, it was shown that the functional murine IL 24(beta)was expressed on normal resting T cells of limited subsets, including CD8^+ qlpha/beta, CD4^-8^- gamma/delta T cells and CD3^- NK cells, but not on the major CD4^+ alpha/beta T cells. Once these T cells are activated by IL 2, they exhibit several characteristic changes in the surface molecules. It was indicated that IL 2-activated T cells but not resting T cells, express high molecular weight Ly 5 isoform. This Ly 5 isofrom was shown to be derived from the full-length, unspliced MRNA as in the case of B cells, resulting in the change in the extracellular domain of-Ly 5 molecules. It was also shown that IL 2-activated T cells rapidly lose the expression of newly defined B7 surface antigen, which is normally expressed in the vast majority of resting T cells. Cloning and expression of B7. cDNA indicated that the B7 antigen was a new member of phosphatidylinositol(PI)-linked molecules. It was thus suggested that IL 2stimulation resulted in the -rapid activation of PI-PLC, which then cleaved. off the surface B7 molecules. Biological significance of the phenomenon remained to be seen. Finally, we have cloned a new gene(spa-1)which was specifically induced by the stimulation of T cells with either IL 2 or anti-TCR antibodies. Spa-1 encoded a 35 Kd protein with many kinase phosphrylation sites and probable DNA binding capacity. Since the expresssion of spa-1 gene showed general correlation with the proliferative status of T cells, it was implied that the product was somehow involved in the regulation of T cell proliferation by lymphokines and antigens.
|
-
-
-
-
-
-
-
[Publications] Hattori, M., Okazaki, H., Ishida, Y., Onuma, M., Kano, S., Honjo, T., and Minato, N.: "Expression of murine IL-2 R beta chain on thymic and splenic lymphocyte subpopulations as recealed by the IL 2-induced proliferative response in human IL-2 Ralpha chain transgenic mice." J. Immunol.144. 3809 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Kubota, H., Okazaki, H., Onuma, M., Kano, S., Hattori, M., and Minato, N.: "Identification and gene cloning of a new phosphatidylinositol-linked antigen expressed on mature lymphocytes." J. Immunol.145. 3924 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sawada, R., Ohsshi, K., Anaguchi, H., Okazaki, H., Hattori, M. n Minato, N., and Naruto, M.: "Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes." DNA and cell biol.9. 213 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Ogawa, Y., Okazaki, H., Sudo, T., Ohno, N., Itho, M., Hattori, M., Iizuka, M., and Minato, N.: "High moleculer weight Ly-5 isoforms expressed on T cells : Activation-dependent expression." Intl. Immunol.3. 39 (1991)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Kubota, H., Okazaki, H., Hattori, M., Kano, S., Onuma, M., and Minato, N.: "CD3+4-8- alpha/beta T cells in the normal bone marrow exhibit biased V beta gene usage. Probable involvement of IL 3 in their generation." J. Immunol.
Description
「研究成果報告書概要(欧文)」より