1991 Fiscal Year Final Research Report Summary
Intercelluar Ca level measured by IP4BP and PI response
| Project/Area Number |
02454250
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAWAGUCHI Hideaki Assistant Hokkaido University School of Medical Hospital faculty, 医学部附属病院, 助手 (70161297)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Inositol-4phosphate / Intracellular Ca / IP4 binding protein / signal transduction / Phospholipse C |
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| Research Abstract |
It is known that inositol (1,4,5)-trisphosphate (IP_3) stimulates Ca^<2+> release from sarcoplasmic reticulum (SR) in several tissues, but in cardiac myocytes the effect of IP_3 on Ca^<2+> release from sarcoplasmic reticulum is controversial. In this experitment, we have proved that (1,4,5)-IP_3 stimulates Ca^<2+> release from cardiac myocyte sarcoplasmic reticulum by digital-image method in signle cell. In cardiac myocyte treated with saponin, Ca^<2+> release stimulated by 10 muM (1,4,5)-IP_3 was detected by fura-2. In ^<45>Ca^<2+> prelabeled sarcoplasmic reticulum, the maximal Ca^<2+> release was achieved at 10 muM IP_3 for 1 min incubation. The release of Ca^<2+> was higher is SHR sarcoplasmic reticulum than in that of WKY.But it is not clear whether this phenomenon has a significant role in cardiac hypertrophy.
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Research Products
(12 results)
