1992 Fiscal Year Final Research Report Summary
Chemotherapy of malignant glioma - understanding of cellular and molecular biology and its clinical application
| Project/Area Number |
02454336
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
ARITA Norio Osaka Univ., Dept. of Neurosurg, 医学部, 助手 (80159508)
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| Co-Investigator(Kenkyū-buntansha) |
平賀 章寿 大阪大学, 医学部, 助手
OHNISHI Takanori Osaka Univ., Dept. of Neurosurg, 医学部, 助手 (70233210)
HAYAKAWA Toru Osaka Univ., Dept. of Neurosurg, 医学部, 教授 (20135700)
HIRAGA Syoju Osaka Univ., Dept. of Neurosurg
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| Project Period (FY) |
1990 – 1992
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| Keywords | brain tumor / glioma / chemotherapy / drug resistance / carcinogensis / p53 / extracellular matrix / tenascin |
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| Research Abstract |
ENU-induced in vitro carcinogenesis of rat fetal astroglia
To investigate glial carcinogenesis in vitro, fetal rat brain cells were cultured and exposed to ENU ( - 200mu g/ml). Cells were passaged weekly thereafter. When mutant colonies appeared. the cells were cloned. Both primary cultured and ENU-treated transformed cells were positive for GFAP and vimentin, but population of A2B5 positive cells was less than 5 %, thus indicating that these cells were astroglial in origin. The mutant colonies appeared 7 weeks after ENU treatment. The cloned mutant glial cell line formed large colonies in low(2%) serum medium and showed tumorigenicity in nude mice. By immunocytochemistry. p53 protein was never detected in non-transformed glial cells, but was positive in ENU-transformed cells. Norhern blot analysis showed over-expression of p53 mRNA in transformed cells. These results indicate that p53 mutation is related to transformation of rat fetal astroglias by ENU in vitro.
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