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1992 Fiscal Year Final Research Report Summary

Surfactant supplement for adult respiratory distress

Research Project

Project/Area Number 02454352
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionKanazawa University

Principal Investigator

KOBAYASHI Tsutomu  School of Med. Anesthe: Associate Prof., 医学部, 助教授 (40019922)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI J  Kanazawa Univ. Hospital: Anesthesiology: Instructor, 医学部・附属病院, 助手 (30227219)
Project Period (FY) 1990 – 1992
KeywordsARDS / Surfactant / Respiratory Failure / Lung Edema / Supplement / Lung Compliance / Hypoxemia
Research Abstract

Pathological process of adult respiratory distress syndrome (ARDS) is largely unknown with 50-70% mortality.
(1) Investigation on the pathological process: An exogenous surfactant was mixed with lung edema fluid sampled from ARDS patients, and administered to the lungs of immature newborn rabbits. When the edema fluid protein-to-surfactant ratio increased from 5.6 to 11.2, the dynamic lung compliance of the animals decreased from 1.08 (mean) to 0.43 ml/cmH_2O/kg (P < 0.05). We conclude that a cause of respiratory failure in ARDS is inactivation of pulmonary surfactant by lung edema that appears during the course.
(2) Treatment of ARDS by surfactant supplement: Respiratory failure induced by administration of E. Coli endotoxin in rats was largely reversed by supplementation of an exogenous surfactant (100 mg/kg); PaO_2 improved from 106 mmHg (mean) to above 400 mmHg (P < 0.05), and diffuse infiltrates in the chest radiogram disappeared. We conclude that surfactant supplementation can reverse the respiratory failure in ARDS.
(3) Artificial surfactant for the supplement therapy: Dipalmitoyl lecithin, unsaturated lecithin (u-L), phosphatidylglycerol and the hydrophobic proteins of porcine surfactant were mixed with various ratios. Dynamic lung compliance of the immature newborn rabbits receiving the mixture consists of all the components was 1.24 ml/cmH_2O/kg (mean), but the value receiving the mixture without u-L was 0.79 ml/cmH_2O/kg (P < 0.05). We conclude that u-L is an important factor for surfactant activity.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kobayashi,Tsutomu: "Exogenous porcine surfactant(Curosurf)is inactivated by monoconal antibody to surfactant-associated hydrophobic protein SP-B." Acta Paediatrica. 81. 665-671 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 陳 衛民: "肺洗淨ラットに対する超音波ネブライザーを用いたサーファクタント補充療法" 日本界面医学会雑誌. 26. 21-28 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 高橋 麗子: "疎水性蛋白質と各種脂質による再構築サーファクタントの生理活性:不飽和ホスファチジルコリンの有用性" 日本界面医学会雑誌. 26. 12-17 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 小林 勉: "肺サーファクタント:その異常が関与する病態" 人工呼吸. 9. 95-100 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Li,Wen-Zi: "Aerosolized surfactant reverses respiratory failure in lung-lavaged rats." Acta Anaesthesiologica Scandinavica. 37. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro,Katsumi: "Surfactant replacement reverses respiratory failure induced by intratracheal injection of endotoxin." Critical Care Medicine. 21. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi,T: "Lung lavage and surfactant replacement for hydrochloric acid aspiration in rabbbits." Acta Anaesthesiol. Scand.34. 216-221 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi,T: "The critical concentration of surfactant in fetal lung liquid at birth." Respir. Physiol.80. 181-192 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Robertson,B: "Structural and functional characterozatopm of porcine surfactant isolated by liquid-gel chromatography." Prog. Respir. Res.25. 237-246 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sun,B: "Aplication of a new ventilator-multi-plethysmograph system for testing efficacy of surfactant replacement in newborn rabbits." Eur. Respir. J.4. 364-370 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi,T: "Activity of pulmonary surfactant after blocking the associated proteins SP-A and SP-B." J. Appl. Physiol.71. 530-536 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Robertson,B: "Experimental neonatal respiratory failure induced by a monoclonal antibody to the hydrophobic surfactant-associated protein SP-B." Pediatr. Res.30. 239-243 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi,T: "In activation of exogenous surfactant by pulmonary edema fluid." Peidatr. Res.29. 353-357 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kurashima,K: "A pilot study of surfactant inhalation for the treatment of asthmatic attack." Jpn. J. Allergol.40. 160-163 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ganzuka,M: "Lung lavage and artificial surfactant replacement therapy: animal experiments and clinical trials for alveolar proteinosis." Jpn. Anaesth. J. Rev.4. 243-246 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi,T: "Exogenous porcine surfactant (Curosurf) is inactivated by monoclonal antibody to the surfactant-associated hydrophobic protein SP-B." Acta Paediatr.81. 665-671 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi,T: "Protein free surfactant composed of phospholipids and tripalmitin: The activity tested in lung-lavaged rabbits." Hokuriku J. Anesth.26. 49-58 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li,W-Z: "Aerosolized surfactant reverses respiratory failure in lung-lavaged rats." Acta Anaesthesiol. Scand.37. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitta,K: "Impairment of surfactant and ventilation by proteins in lung edema fluid." Respir. Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro,K: "Surfactant replacemenet reverses respiratory failure induced by intratra-cheal injection of endotoxin in rats." Critical Care Med.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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