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1991 Fiscal Year Final Research Report Summary

Strain Variation of Candida albicans as Related to Pathogenicity to the Urinary Tract

Research Project

Project/Area Number 02454369
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionSchool of Medicine, Kanazawa University

Principal Investigator

OHKAWA Mitsuo  Department of Urology, School of Medicine, Kanazawa University, Associated Professor, 医学部, 助教授 (80019596)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Takao  Department of Urology, Kanazawa University Hospital, Research Associate, 医学部附属病院, 助手 (20217706)
TOKUNAGA Shuji  Department of Urology, Kanazawa University Hospital, Research Associate, 医学部附属病院, 助手 (70207554)
Project Period (FY) 1990 – 1991
KeywordsCandida albicans / Strain variation / Serotype / Mycelial form / Urinary candidiasis / IC_<30> / _D-arabinitol / Mannan
Research Abstract

Although candiduria is not an infrequent finding, its clinical significance is uncertain. Therefore, we studied urinary candidiasis experimentally and clinically. Among Candida species, Candida albicans was the most frequently isolated from clinical specimens, followed by Candida tropicalis and Candida glabrata. Serotyping of the C. albicans isolates demonstrated that serotype A strains were more frequently isolated than serotype B ; the isolates of C. albicans serotype B showed a significantly lessened susceptibility to 5-fluorocytosine than those of serotype A, by means of a photo-read broth microdilution method for determination of 30% inhibitory concentration (IC_<30>) . We found that mitochondrial DNA restriction profiles of C. albicans strains isolated from urine specimens can be divided into several types. Ascending pyelonephritis in rats with incomplete ureteral obstruction, developed at a high rate, using both C. albicans serotype A strain and B strain ; the incidences were si … More milar. On the other hand, the incidence of ascending pyelonephritis using a C. glabrata strain and a mutant strain of C. albicans. which are not dimorphic and occur only as yeast cells, decreased significantly compared with those using C. albicans strains. In addition, germinated C. albicans cells were observed to adhere to the urothelium of the rat bladder more easily than yeast-form organisms. These results strongly suggest that the mycelial form is important for tissue invasion to the urinary tract. A clinical study of candiduria patients revealed that disturbance of urine flow, antimicrobial chemotherapy and indwelling urinary catheters are important factors predisposing to candiduria. We demonstrated that there are statistically significant relationships between renal. and urinary Candida colony counts, and between renal Candida populations and grades of renal histological changes, using two new experimental models of ascending. C. albicans pyelonephritis in rats. Based on our experimental and clinical data, serum _D-arabinitol and mannan are considered to be useful markers in distinguishing invasive urinary candidiasis form benign colonization. The data on the serum _D-arabinitol/creatinine ratios in urinary candidiasis patients suggest that 2 weeks or more may be an appropriate duration of treatment for urinary candidiasis. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 大川 光央,徳永 周二: "原因菌別にみた尿路感染症:Candida spp." 泌尿器外科. 4. 345-352 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] S.Tokunaga,M.Ohkawa,M.Takashima,H.Hisazumi: "Clinical significance of mesasurement of serm -arabinitol levels in cadiduria patients" Urologia Intenationalis.

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      「研究成果報告書概要(和文)」より
  • [Publications] S.Tokunaga,M.Ohkawa,T.Nakashima,H.Hisazumi: "Candida antigen detection by a latex agglutination test in candiuria patients" Urologia Internationlis.

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      「研究成果報告書概要(和文)」より
  • [Publications] S.Tokunaga,M.Ohkawa,H.Yamamoto M.Takashima,H.Hisazumi: "Caluculus pyelonephritis due to Candida albicans with special reference to serodiagnostic parameters" Journal of Japanese Association for Infectious Disease.

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      「研究成果報告書概要(和文)」より
  • [Publications] 高島 三洋: "水腎症ラットにおける上行性カンジタ腎孟腎炎の実験的研究" 十全医会誌.

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      「研究成果報告書概要(和文)」より
  • [Publications] M.Ohkawa,S.Tokunaga: "Clinical significance of Candiduria:an update" International Urogynecology Journal.

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      「研究成果報告書概要(和文)」より
  • [Publications] Ohkawa, M., tokunaga, S., Shoda, R., Hisazumi, H.: "Clinical significance of the antibody-coated bacteria test in patients with candiduria." Br. J. Urol.66. 22-25 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M., Sugata, T., Sawaki, M., Nakashima, T., Fuse, H., Hisazumi, H.: "Bacterial and crystal adherence to the surfaces of indwelling urethral catheters." J. Urol.143. 717-721 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M., Tokunaga, S., Takashima, M., Nishikawa, T., Hisazumi, H. Fujita, S., Wheat, W. R.: "In Vitro susceptibility testing of Candida isolates from clinical specimens to four antifungal agents." Chemotherapy (Basel). 36. 396-402 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, S., Ohkawa, M., Takashima, M., Hisazumi, H.: "A clinical study of bacteremia in urology." J. Jpn. Assoc. Infect. Dis.64. 661-667 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M., Tokunaga, S.: "Urinary tract infection caused by respective pathogens : Candida spp." Jpn. J. Urol. Surg.4. 345-352 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M.: "Bacterial adherence to Foley urinary catheters." Int. Urogynecol J.2. 236-241 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M., Tokunaga, S., Takashima, M., Hisazumi, H.: "Candida urinary tract infection with special reference to ascending pyelonephritis." Acta Urol. Jpn.37. 969-974 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, S., Ohkawa, M., Takashima, M., Hisazumi, H.: "Clinical significance of measurement of serum _D-arabinitol levels in candiduria patients." Urol. Int.

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takashima, M.: "An experimental study of ascending Candida albicans pyelonephritis with open hydronephrosis." J. Juzen Med. Soc.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, S., Ohkawa, M., Nakashima, T., Hisazumi, H.: "Candida antigen detection by a latex agglutination test in candiduria patients." Urol. Int.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkawa, M., Tokunaga, S.: "Clinical significance of candiduria : an update." Int. Urogynecol., J.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, S., Ohkawa, M. Yamamoto, H., Takashima, M., Hisazumi, H.: "Calculus pyelonephrisis due to Candida albicans with special reference to serodiagnostic parameters." J. Jpn. Assoc. Infect. Dis.

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      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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