1992 Fiscal Year Final Research Report Summary
Molecular biological study on genesis and proliferation of renal malignant tumor
| Project/Area Number |
02454373
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
HAYASHI Satoru Keio Univ., Sch. of Med. Dept. of Urology, Assistant, 医学部, 助手 (60180965)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Makoto Tokyo Dental College. Dept. of Urology, Professor, 歯学部, 教授 (40051586)
SAITO Shiro Keio Univ., Sch. of Med. Dept. of Urology, Assistant, 医学部, 助手 (80170504)
BABA Shiro Keio Univ., Sch. of Med. Dept. of Urology, Assistant Professor, 医学部, 講師 (00051889)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Renal Cell Carcinoma / Chromosom / G-banding technique / Transitional Cell Carcinoma / H-ras oncogene / Point Mutation |
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| Research Abstract |
We started this study in aiming to clarify the mechanism of genesis and proliferation of renal malignant tumors by means of molecular biology technique utilizing tissue materials surgically extirpated from the patients with renal cell carcinoma.
In the 1st year, chromosomal analysis was carried out on 8 tumors of renal cell carcinoma. There were relatively high incidence of numerical aberrations in #3 and #7 chromosoms, and a lesser incidence of constitutional aberrations, i.e. long-arm aberrations in #2 and #6 chromosoms.
In the 2nd year, DNAs were extracted from these 8 tumors and then subjected to evaluation of various oncogene expression. However, no significant abnormaility was found. On the other hand, it was successful to detect over expression of H-ras oncogene and its point mutation by means of polymerase chain reaction(PCR) method in 2 out of 50 bladder cancers (4%). In this method, the DNA segments including codon 12 were amplified by PCR, and the reaction products were examined for their susceptibility to the restriction enzyme, and by dot blot hybridization assay with oligonucleotide probes.
Point mutations were detectable in small amounts of DNAs isolated from fresh or frozen tumor tissues, urine cells and paraffin sections. These results indicated that our method was not superior as screening,but very useful in following up the readily positive cases. Particularly emphasized is feasibility of urine cells as test samples because of their non invasiveness to the patients and easy repeatability.
In the 3rd year, we made efforts to try to publicate our results.
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[Publications] Hata,M., Baba,S., Saito,S., Tachibana,M., Deguchi,N., Jitsukawa,S. and Tazaki,H.: "Chromosomal analysis of renal cell carcinoma" Jpn. J. of Urology. 81(9). 1389-1395 (1990)
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「研究成果報告書概要(欧文)」より
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[Publications] Hata,M., Baba,S., Tachibana,M., Deguchi,N., Jitsukawa,S. and Tazaki,H.: "Kidney preserving surgery for renal cell carcinoma in patients with a solitary kidney or bilateral renal tumors" Jpn. J. of Urology. 82(3). 412-419 (1991)
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[Publications] Nakashima,J., Tachibana,M., Baba,S., Deguchi,N., Jitsukawa,S., Hata,M., and Tazaki,H.: "Studies on the production of endogenous cytokines in patients with renal cell carcinoma" Jpn. J. of Urology. 83(7). 1110-1117 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Saito,S., Makoto,H., Fukuyama,R., Sakai,K., Kudoh,J., Tazaki,H. and Shimizu,N.: "Detection of H-ras gene point mutations in 50 cases of bladder carcinoma" Cancer Research.
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「研究成果報告書概要(欧文)」より
