1991 Fiscal Year Final Research Report Summary
Genetic control of experimental autoimune uveitis in the mouse
| Project/Area Number |
02454401
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kurume University School of Medicine |
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Principal Investigator |
MOCHIZUKI Manabu Department of Ophthalmology, Kurume University School of Medicine, 医学部, 教授 (10010464)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASAKI Hiroshi Department of Ophthalmology, Kurume University School of Medicine, 医学部, 助手 (90172519)
HIKITA Naofumi Department of Ophthalmology, Kurume University School of Medicine, 医学部, 助手 (80173152)
IWAMI Hiroko Department of Ophthalmology, Kurume University School of Medicine, 医学部, 助手 (30151757)
NISHIDA Hiroshi Department of Ophthalmology, Kurume University School of Medicine, 医学部, 講師 (30131775)
SHIOTANI Nobuyuki Department of Ophthalmology, Kurume University School of Medicine, 医学部, 講師 (90113224)
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| Project Period (FY) |
1990 – 1991
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| Keywords | mouse / retinal soluble antigen / autoimmune uveitis / B10 congenic mouse / Hー2ハプロタイプ |
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| Research Abstract |
A model of experimental autoimmune uveitis(EAU)in the mouse was established by an enhanced immunization(2-4 times injection at an interval of 2-4 weeks)with S-antigen or interphotoreceptor retinoid binding protein(IRBP)using Krebsielia lipopolysaccharide(K03 LPS)as an adjuvant. The incidence of EAU was tested in several strains of B10. BR, B10. A, B10. MBR, B10. A(4R)were found to be high responders to S-antigen-induced EAU as well as to IRBP-induced EAU. On the other hand, B10. A(5R)was non-responder to S-antigen-induced EAU and low-responder to IRBP-induced EAU. These data thus indicate that the susceptibilty to EAU in the mouse was restricted by the immune response genes(H-2), particulady by 1-A^k. The susceptibility to EAU in AKR/J was very low, although H-2 haplotype ws identical to B10. BR. Therefore, it is suggested that non-MHC genes also plays some minor role in EAU.
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