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1991 Fiscal Year Final Research Report Summary

Studies on the mechanism of leucocytosis and hypercalcemia associated with head and neck cancer

Research Project

Project/Area Number 02454457
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionOsaka University

Principal Investigator

SAKUDA Masayoshi  2nd Dept. of Oral-and Maxillofacial Surg. Facul. of Dentist., Osaka Univ. ; Professor, 歯学部, 教授 (00028755)

Co-Investigator(Kenkyū-buntansha) OHMAE Masatoshi  2nd Dept. of Oral-and Maxillofacial Surg. Fucul. of Dentist., Osaka Univ. ; Resi, 歯学部附属病院, 医員
YOSHIKAWA Fumihiro  2nd Dept. of Oral-and Maxillofacial Surg. Facul. of Dentist., Osaka Univ. ; Assi, 歯学部, 助手 (80183698)
NAKAZAWA Mitsuhiro  2nd Dept. of Oral-and Maxilliofacial Surg. Facul. of Dentist., Osaka Univ. ; Ass, 歯学部附属病院, 助手 (70217701)
Project Period (FY) 1990 – 1991
KeywordsHead and Neck Cancer / leucocytosis / hypercalcemia / paraneoplastic Syndrome
Research Abstract

In this studies, the mechanism underlying leucocytosis and hypercalcemia associated with malignancy was investigated.
In this investigation of leucocytosis and/or hypercalcemia occasionally occurred in patients with oral malignancies, some patients have only one of them, whereas others have both. These results demonstrate that these paraneoplastic syndromes result from different mechanisms.
In accordance with this fact, tongue cancer of a patient with hypercalcemia was inoculated into nude mice and, at the same time, cultured in vitro. These tumor cells secrete high level of PGE_2, which is revealefl, to play important roles in bone resorption. Furthermore, high level of PGE_2 or Ca^<2+> in culture medium promoted DNA synthesis of tumor cells. In the further studies used VX_2 cancer cells, the cells secreted PGE_2 and also parathyroid hormone-related protein (PTHrP). These humoral factors are involved in pathogenesis of hypercalcemia. Increase of serum PGEP_2 and PTHrP results in enlargement of bone marrow cavity of parietal bone. Administration of indomethacin or antagonist of PTHRP, PTHRP analogue, prevented a bone resorbing activity of these factors. Both PGE_2 and PTHRP promoted Vxg cell growth as autocrine growth factors. Stimulation of VX_2 cell proliferation by PGE_2 or ETHRP was closely correlated with a transient elevation of intracellular free Ca^<2+> by influx of extracellular free Ca^<2+>.
It is, therefore, suggested that induction of the hypercalcemia is an essential event for VX_2 cells to maintain an environment which favors their autonomous growth.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 中澤 光博、他: "頭頚部扁平上皮癌患者の臨床的研究ー高齢者と若壮年者の比較検討ー" 日本口腔外科学会雑誌. 36. 94-100 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Yoneda,et al.: "Freqency of Hypercalcemia-Leukocytosis Syndrome in Oral Malignancies" Cancer. 168. 617-622 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Yoneda,et al.: "Occurance of hypercalcemia and Leukocytosis with Cachexia in Human Squamous Carcinoma of the Maxilla in Athymic Mude Mice:A Novel Experimetal model of Three Concomitant Paraneoplastic Syndromes" Journal of Clinical Oncology. 9. 468-477 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中澤 光博、他: "下顎歯肉扁平上皮癌の顎骨吸収様式と予後との関連性" 日本口腔科学会雑誌. 40. 589-599 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fumihiro Yoshikawa,et al.: "Production of Self-Migration Stimulating Factor by Metastatic Human malignant Melanoma Cells Established in Culture" Research Communications in Chemical Pathology and Pharmacology. 73. 21-29 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川 文弘、他: "口蓋原発悪性黒色腫由来の初代培養細胞の性状について" 日本口腔外科学会雑誌. 37. 1797-1801 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhiro Nakazawa, et al.: "The clinical study of head and neck squamous cell carcinoma Examination of the young patients and aged patients" Jpn. J. Oral Maxillofacial. Surg.36. 1296-1307 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Yoneda, et al.: "Frequency of Hypercalcemia-Leukocytosis Syndrome in Oral Malignancies" Cancer. 168. 617-622 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Yoneda, et al.: "Occurance of hypercalcemia and Leukocytosis with Cachexia in a human squamous Carcinoma of the Maxilla in Athymic Nude Mice : A Novel Experimental model of Three Concomitant Paraneoplastic Syndromes" J. Clinical Oncology. 9. 468-477 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhiro Nakazawa et. al.: "The Relationship between Prognosis and the Mode of Bone Resorption by Carcinomas Involving the Mandible" J. Jpn. Stomatol. Soc.40. 589-599 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumihiro Yoshikawa et al.: "Production of Self-Migration Stimulating Factor by Metastatic Human malignant Melanoma Cells Established in Culture" Res. Commun. in Chemical Pathology and Pharmacology. 73. 21-29 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumihiro Yoshikawa et al.: "Cheractor of primary culture cells derived from oral malignant melanoma" Jpn. J. Oral Maxillofacial. Surg.37. 1797-1801 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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