1992 Fiscal Year Final Research Report Summary
CHROMOSOME MAPPING OF MUTANT GENE CONCERNING GENETIC DISEASE IN MICE USING DNA POLYMORPHISMS
| Project/Area Number |
02454526
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY |
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Principal Investigator |
WATANABE Tomomasa INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY, DEPARTMENT OF BIOCHEMISTRY, HEAD OF GROUP, 生化学部, 室長 (10100174)
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| Co-Investigator(Kenkyū-buntansha) |
MASAKI Shigeo INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY, INSTITUTE FOR DEV, 生化学部, 主任研究員 (10157175)
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| Project Period (FY) |
1990 – 1992
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| Keywords | mouse / DNA polymorphism / RFLP / chromosome mapping / backcross / MRL strain / autoimmune disease / linkage |
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| Research Abstract |
The lpr (lymphoproliferation) gene, which is an autosomal recessive gene causing autoimmune disease with features of human systemic lupus erythematosus and iventually death from severe immune-complex glomerulonephritis, has been mapped on mouse chromosome 19. To determine the exact chromosomal location of the lpr gene, the interspecific backcross was carried out by the mating (MRL/MpJ-lpr/lpr x MOL-TIT)x MRL/MpJ-lpr/lpr. Ly-44 (lymphocyte differentiation antigen-44) and Tdt (terminal deoxynucleotidyl transerase) were used as molecular marker genes on chromosome 19. RFLP of Ly-44 (lymphocyte differentiation antigen-44) and Tdt were in the case of BamHI. The following order of genes is proposed with the distances between genes given in parenthese: centromere - Ly-44 - (19.3cM) - lpr - (6.1cM) - Tdt - telomere. Therefore, the lpr gene is mapped at position 23 on mouse chromosome 19.
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[Publications] Sakai,Y.,Miyawaki,S.,Shimizu,A.,Ohno,K.and Watanabe,T.: "A mdacular genetic linkage map of mouse chromosome 18,including spm,Grl-1,Fim-21c-fms,and Mbp." Biochemical Geneties. 29. 103-113 (1991)
Description
「研究成果報告書概要(和文)」より
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[Publications] Watanabe,T.,Sakai,Y.,Miyawaki,S.,Shimizu,A.,Koiwai,O.and Ohno,K.: "A mdacular genetic linkage map of mouse chromosome 19,induding the lpr,Ly-44,and Tdt genes." Biochemical Geneties. 29. 325-335 (1991)
Description
「研究成果報告書概要(和文)」より
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[Publications] Shimizu,A.,Sakai,Y.,Chno,K.,Masaki,S.,Kawano,R.,Takahashi,Y.,Miyaxhita,N.and Watanabe,T.: "A molecular genetic linkage map of mouse chromosome 10,including the Myb,S100b,Pah,Sl.and IFg genes." Biachemical Genetics. 30. 529-535 (1992)
Description
「研究成果報告書概要(和文)」より
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[Publications] Watanabe, T., Ohno, K., Shimizu, A., Sakai, Y., Takahashi, M. and Takahashi, N.: "Mapping of the Hox-3.1 and Myc-1.2 genes on chromosome 15 of the mouse by restriction fragment length variations." Biochemical Genetics. 28. 257-266 (1990)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sakai, Y., Miyawaki, S., Shimizu, A., Ohno, K. and Watanabe, T.: "A molecular genetic linkage map of mouse chromosome 18, including spm, Gr1-1, Fim-2/c-fms, and Mbp." Biochemical Genetics. 29. 103-113 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Watanabe, T., Sakai, Y., Miyawaki, S., Shimizu, A., Koiwai, O. and Ohno, K.: "A molecular genetic linkage map of mouse chromosome 19, including the lpr,Ly-44, and Tdt genes." Biochemical Genetics. 29. 325-335 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shimizu, A., Sakai, Y., Ohno, K., Masaki, S., Kuwano, R., Takahashi, Y., Miyashita, N. and Watanabe, T.: "A molecular genetic linkage map of mouse chromosome 10, including the Myb, S100b, Pah, Sl, and Ifg genes." Biochemical Genetics. 30. 529-535 (1992)
Description
「研究成果報告書概要(欧文)」より
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