1992 Fiscal Year Final Research Report Summary
Genetic Ecology of Bradyrhizobium japonicum in soils
| Project/Area Number |
02455007
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | IBARAKI UNIVERSITY |
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Principal Investigator |
ASAMI Teruo IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: PROFESSOR, 農学部, 教授 (20011877)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMISAWA Kiwamu IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: ASSOCIATE PROFESSOR, 農学部, 助教授 (70167667)
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| Project Period (FY) |
1990 – 1992
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| Keywords | Bradyrhizobium / root nodule / soybean / host specificity / fingerprint / signal / genetic ecology |
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| Research Abstract |
The isolates of Bradyrhizobium japonicum indigenous to six field in Japan were characterized by using four DNA hybridization probes from the species; nifDK, nodDYAB, hup, RS-alpha, and RS- beta. Diversity in RS-specific hybridization (RS-fingerprint) were observed. The RS-fingerprints were correlated with the nif and hup genotypes, and with the location of field including cropping history. According to nodDYAB hybridization, the field isolates were classified into three main group A, B and C. The group A and B produced similar nod metabolites including NodBj-V with methyl fucose. The group C isolates, which were separated only from siratro, were incompatible with soybeans,and produced different nod metabolites. As for nodulation, we constructed simple relationship. As a result, we established the method of genetic ecology for indigenous bradyrhizobia.
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