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1992 Fiscal Year Final Research Report Summary

Genetic Ecology of Bradyrhizobium japonicum in soils

Research Project

Project/Area Number 02455007
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionIBARAKI UNIVERSITY

Principal Investigator

ASAMI Teruo  IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: PROFESSOR, 農学部, 教授 (20011877)

Co-Investigator(Kenkyū-buntansha) MINAMISAWA Kiwamu  IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: ASSOCIATE PROFESSOR, 農学部, 助教授 (70167667)
Project Period (FY) 1990 – 1992
KeywordsBradyrhizobium / root nodule / soybean / host specificity / fingerprint / signal / genetic ecology
Research Abstract

The isolates of Bradyrhizobium japonicum indigenous to six field in Japan were characterized by using four DNA hybridization probes from the species; nifDK, nodDYAB, hup, RS-alpha, and RS- beta. Diversity in RS-specific hybridization (RS-fingerprint) were observed. The RS-fingerprints were correlated with the nif and hup genotypes, and with the location of field including cropping history. According to nodDYAB hybridization, the field isolates were classified into three main group A, B and C. The group A and B produced similar nod metabolites including NodBj-V with methyl fucose. The group C isolates, which were separated only from siratro, were incompatible with soybeans,and produced different nod metabolites. As for nodulation, we constructed simple relationship. As a result, we established the method of genetic ecology for indigenous bradyrhizobia.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kiwamu Minanisawa: "Genetic Relatedness of Bradyrhizobium japonicum Field Isolates as Revealed by Repeated Saguencos and Various Other Characteristics" Applied and Environmental Microbiology. 58. 2832-2839 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 南沢 究: "インドール酢酸生産能によるダイズ根粒菌の系統分類法" 日本土壌肥料学雑誌. 63. 459-462 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 南沢 究: "ダイズ根粒菌の系統と生態" 土の微生物. 40. 37-46 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Minamisawa, T. Seki, S. Onodera, M. Kubota, T. Asami: "Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics" Appl. Environ. Microbiol.58. 2832-2839 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Minamisawa, H. Iino, N. Saito, S. Onodera, Y. Nakatsuka, S. Nakazima, M. Kubota, T. Asami: "Determination of genotype group of Bradyrhizobium japonicum by indole-3-acetic acid production." Jpn. J. Soil Sci. Plant Nutr.63. 459-462 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Minamisawa: "Phylogeny and ecology of Bradyrhizobium japonicum" Soil Microorganisms. 40. 37-46 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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