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1992 Fiscal Year Final Research Report Summary

Detection of Beneficial Nematodes in Japan and Systematized Nematode Application

Research Project

Project/Area Number 02506001
Research Category

Grant-in-Aid for Developmental Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionSaga University

Principal Investigator

ISHIBASHI Nobuyoshi  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (30039333)

Co-Investigator(Kenkyū-buntansha) HATSUKADE Masayoshi  Shizuoka University, Faculty of Agric., Professor, 農学部, 教授 (40091152)
IWAHANA Hidenori  Tokyo University of Agric. & Technology, Professor, 農学部, 教授 (90038240)
SHONO Toshio  Tsukuba Univ., Inst. of Agric. & Forestry. Professor, 農学部, 教授 (80011922)
TOJO Sumio  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (50011911)
KONDO Eizo  Saga University, Faculty of Agric., Associate Prof., 農学部, 助教授 (60039336)
Project Period (FY) 1990 – 1992
Keywordsbeneficial nematodes / biological control / entomopathogenic nematodes / fungivorous nematodes / mass-production / mixed application / nictating behavior / symbiotic bacteria
Research Abstract

Entomopathogenic nematodes :
1) Steinernematid nematodes distribute throughout Japan, while heterorhabditids localize in subtropical (Okinawa) and warm temperate area along the Pacific coast. These beneficial nematodes were found in woodlands and grasslands rather than from tilled fields.
2) Life cycle of a tylenchid nematode parasitizing reproductive organs of the adult Japanese pine sawyer was clarified. The nematode induces mock oviposition to adult females of the insect, eventually bringing the decline of the population density of insects. Fungivorous stages of this nematode can be mass-produced with a fungus growing on artificial medium. The inseminated adult females penetrate the larvae of the beetle, maturating synchronizingly with the gonadal development of the rost insect, and lay eggs in the host ovaries.
3) Host-finding behavior of S. carpocapsae was investigated. A simple and reprodicible bioassay was established by using exshathed infective juveniles and insect plasma. This b … More ioassay reflects the host preference or infectivity of nematodes. The attractive agent in host insect plasma was determined to be a peptide.
4) Factors affecting the growth of steinernematid nematodes were investigated. The nematodes can continue the development of the 1st the generation-type adults as long as they ate transferred individually onto fresh medium.
5) Both infective juveniles and their symbiotic bacteria destroy the defense mechanism of host insects ; both of the two agents suppress prophenol oxidase cascade activation and cellular defense in the larval haemolymph of insects.
6) Nictating juveniles killed host insects faster than did non-nictating ones. Insecticticides that enhance nictating behavior at certain concentrations can be used for mixed application with nematodes for the integrated control of insect pests. Some insecticides are non-toxic to nematodes at 100 ppm some enhance the nictating behavior, suggesting the compatibility with nematodes.
7) Antibacterial and protease activity of symbiont bacteria are responsible for the pathogenicity of entomopathogenic nematodes. The symbiont bacteria with high metabolic activity can be isolated, leading to the mass-production of nematodes with high killing-activity against insects.
Fungivorous nematode, Aphelenchus avenae: For the mass production of nematodes, the mixture (1:1) of dregs from orange juice production and wastepotatoes from potato chips production was the best substrate for nematodes and fungi as food. This mixture (8g dw) with 60% water content produced 5x10^5-1x10^6 nematodes 30 days after inoculation with100 nematodes. Maintenance of nematodes as seed should be conducted by changing the fungus species as the subculture host. B.cinerea (non-vilulent isolate) is recommended as the food of nematodes at the final production for use. In soil application of A. avenae (5x10^4/185 ml soil/pot) for controlling pre-emergence damping-off of cucumber seedlings caused by R. solani AG-4, F. oxsporum f. sp. lagenariae, Pythium sp., Phytophthora nicotiana var. parasitica, there were no significant differences in the controlling efficacy of nematodes. giving 81-85% germination, while each fungus species alone without nematodes gave no germination. Less

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Khlibsuwan,W.,Ishibashi,N.,and Kondo,E.: "Effects of enzymes,chemicals,and temperatura on Steinernema carpocapsae attraction to host plasma." Journal of Nematoloqy(USA). 24. 482-488 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Khlibsuwan,W,Ishibashi,N.,and Kondo,E.: "Response of Steinernema carpocapsae infective juveniles to the plasma of three insect species." Journal of Nematoloqy(USA). 24. 156-159 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi,N.,and Takii,S.: "Effects of insecticides on movement,nictation,and infectivity of Steinernema carpocapsae" Journal of Nematoloqy(USA). 25. 204-213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi,N.,and Choi,D-R.: "Bioloqical of soil pests by mixed application of entcmopathoqenic and fungivorous nematodes." Joural of Nematoloqy(USA). 23. 175-181 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Choi,D-R.,and Ishibashi,N.: "Life-cycle parameters of Aphelenchus avenae on Botrytis cinerea and Fusarium oxysporum." Japanese Journal of Nematoloqy. 21. 1-5 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoo,S.,Tojo,S.,and Ishibashi,N.: "Suppression of the prophenoloxidase in the larval haemolymph of the turnip moth,Agrotis segetum,by the entomopathogenic nematode and its symbiont bacterium." Journal of Insect Physioloqy(UK). 38. 915-924 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柏尾具後: "ハスモンヨトウに対するSteineruemd carpocapsaeの殺虫効果に及ぼす線虫散布後の温湿度条件の影響" 九州病害虫研究会報. 38. 136-138 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamanaka,Hagiwara,Nishimura,Tanabe,and Ishibashi: "Biochemical and physiological characteristics of Xenorhabdus spp.,symbiotically associated with entomopathogenic nematodes including Steinernema Kushidai." Archives of Microbiology(Germany). 158. 387-393 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo,E.,and Ishibashi,N.: "Dependency of three steinernematid nematodes on their symbiotic bacteria for growth and propagation." Japanese Journal of Nematology. 21. 11-17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,Hori,Asano,Ogiwara,Sato,Ohba,and Iwahana: "Insecticidal spectrum of a novel isolate of Bacillus thringiensis Serovar.japonensis." Biological Control(USA). 2. 138-142 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogura,N.,and Kosaka,H.: "Life cycle of Contortylenchus sp.associated with the Japanese pine sawyer beetle." Proceeding of the 19th IUFRO World Congress.1990.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogura,N.,and H.Kosaka: "Biology of a tylenchid nematode parasitic on the Japanese pine sawyer,Monochamus alternatus." Nematologica(The Netherland). 37. 455-469 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi,Nibuyoshi: "Nematodes and Biological Control of Insect Pestsed.by R.Bedding,R.Akhurst,and H.K.Kaya" CSIRO,Australia, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi,N.and Kondo,E.: "Entomopathogenic Nematodes in Biological Controled.by R.Gaugler and H.K.Kaya" ORC,Boca Raton,FL., 365 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩花秀典(編者): "バイオ農薬,水産薬の開発と利用(江草.駒田.岩花編)" CMC,東京, 334 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石橋信義: "“昆虫寄生性線虫"「バイオ農薬,水産薬の開発と利用」" CMC.東京, 334 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi, N., and S. Takii: "Effects of insecticides on movement, nictation, and infectivity of Steinernema carpocpasae." J.Nematol (USA). 25. 204-213 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Khlibsuwan, W., N. Ishibashi, and E. Kondo: "Effects of Enzymes, Chemicals, and temperture on Steinernema carpocapsae." J.Nematol. 24. 482-488 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Khlibsuwan, W., N. Ishibashi, and E. Kondo: "Response of Steinernema carpocapsae infective juveniles to the plasma of three insect plasma." J.Nematol. 24. 156-159 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Khlibsuwam, W., and N. Ishibashi: "Evidences and partial characterization of entomopathogenic nematodes' attractant in host insect plasma." J.Nematol. 25.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishibashi, N., and D-R. Choi: "Biological control of soil pests by mixed application of entomopathogenic and fungivorous nematodes" J.Nematol. 23. 175-181 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Choi, D-R., and N. Ishibashi: "Life-cycle parameters of Aphelenchus avenae on Botyutis cinerea and Fusarium oxysporum." Japn. J Nematol. 21. 1-5 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoo, S., S. Tojo, and N. Ishibashi: "Suppression of the prophenoloxidase cascade in the larval haemolymph of the turnip moth, Agrotis segetum, by the entomopathogenic nematode, Steinernema carpocapsae, and its symbiont bacterium, Xenorhabdus nematophilus." J. Insect Physiol (UK). 38. 915-924 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamanaka, S., A. Hagiwara, Y. Nishimura, H Tanabe,and N. Ishibashi: "Biochemical and physiological characteristics of Xenorhabdus species symbiotically associated with entomopahogenic nematodes including Steinernema kushidai and their pathogenicity against Spodoptera litura (Lepodoptera : noctuidae)" Arch. Microbiol.158. 387-393 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, E., and N. Ishibashi: "Dependency of three steinernematid nematodes on their symbiotic bacteria for growth and propagation." Japn. J. nematol.21. 11-17 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogura, N., and H. Kosaka: "Biology of a tylenchid nematode parasitic on the Japanese pine sawyer, Monochamus alternatus" Nematologica. 37. 455-469 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishibashi. N.: Integrated Control of Soil Pests by Steinernema carpocapsae.in "Nematodes and Biological Control of Insect Pests". ed by R. Bedding, R. Akhurst, and H. K. Kaya., (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishibashi, N., and E. Kondo.: Bebavior of Infective Juveniles in "Entomopathogenic Nematodes in Biological Control" ed. by R. Gaugler and H. K. Kaya., 365 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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