1992 Fiscal Year Final Research Report Summary
Immunohistochemical analysis of neuronal networks in brain using antibody for neurotransmitter receptors
| Project/Area Number |
02557011
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
KURIYAMA Kinya Kyoto Pref.Univ.of Med.Pharmacol.,Professor, 医学部, 教授 (20079734)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATSURA Masashi Kyoto Pref.Univ.of Med.Pharmacol.,Instructor, 医学部, 助手 (80204452)
HIROUCHI Masaaki Kyoto Pref.Univ.of Med.Pharmacol.,Instructor, 医学部, 助手 (70181196)
TAGUCHI Jun-ichi Kyoto Pref.Univ.of Med.Pharmacol.,Instructor, 医学部, 助手 (50188132)
|
|---|
| Project Period (FY) |
1990 – 1992
|
| Keywords | GABA_Areceptor antibody / Receptor purification / GABA_B_receptor antibody / Monoclonal antibody / Baclofen affinity column / Immunoaffinity column / Immunohistochemistry / Electron Microscopic immuno-cytochemistry |
|---|
| Research Abstract |
Immunohistochemical procedures for cerebral GABA_A and GABA_B receptors using specific monoclonal antibodies have been developed. The results obtained are as follow. 1)Albin o rabbits were immunized against partially purified GABA_A receptor complex from the bovine brain using baclofen-affinity column, and the specific antibody for GABA_A receptor complex was obtained. 2) Mice were immunized against partially purified GABA_B receptor protein using baclofen-affinity column, and the monoclonal antibody which recognizes 80KDa GABA_B receptor was obtained. 3) The immunoaffinity column using GABA_B receptor monoclonal antibody was obtained, and complete purification of the GABA_B receptor protein(80KDa) was achieved using this immunoaffinity column. 4) The purified GABA_B receptor possessed pharmacological properties as GABA_B receptor and exhibited the inhibition of cAMP formation when it was reconstituted in phospholipid vesicles with purified adenylate cyclase and Gi/Go type of GTP binding protein. 5) Immunohistochemical studies using above GABA_A receptor and GABA_B receptor antibodies revealed that the localization of GABA_A receptor and GABA_B receptors at GABAergic synapses was different. 6) It has been confirmed that these immunohistochemical procedures are useful for the analysis of GABAergic pathways in the brain.
|
