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1992 Fiscal Year Final Research Report Summary

Studies on thrombolytic enzyme nattokinase

Research Project

Project/Area Number 02558013
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionDepartment of Nutrition & Food Science , Okayama Prefectural Junior College

Principal Investigator

SUMI Hiroyuki  Okayama Prefectural Jun. College, Nutrition, Associate Prof., 食物科, 助教授 (00107814)

Co-Investigator(Kenkyū-buntansha) AKAGI Reiko  Rockefeller University, Medicine, Associate Prof, 食物科(ロックフェラー大学医学部), 助教授 (50150967)
H P Klocking  独国, 国立医科研究所, 教授
NAKAJIMA Nobuyoshi  Okayama Prefectural Jun.College, Nutrition, Acistant Prof., 食物科, 講師 (10198070)
HAMADA Hiroki  Okayama University of Science, Natural Science, Associate Prof., 理学部・基礎理学科, 助教授 (10164914)
KLOCKING H P  Medical Academy Erfurt. Pharmacology, Prof.
Project Period (FY) 1990 – 1993
KeywordsNattokinase / Thrombolytic enzyme / Fibrinolysis / Ferm entation / Natto
Research Abstract

Nattokinase (NK) was prepared from the saline extract of natto as reported previously (H. Sumi et al., Experientia 43: 1110, 1987; Thromb. Haemostas. 62: 5409, 1989). The mol. wt. and pI were about 20,000 and 8.6, respectively. NK not only digested fibrin (with or without plasminogen) but also the plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251). While not reacting with the plasmin titrator p-nitrophenyl-guanidnobenzoate, purified NK reacted with N-trans-cinnamoylidazole, a papain or chymotrypsin titrator, 53.0 % active per molecule.
The amino acid sequence of the purified preparation was determined from 9 peptides obtained by Lys-endopeptidase treatment ; NK was found to be a serine protease consisting of 275 residues with Ala at the N-terminal, and possessing no S-S molecular bond, in which it differs from previously known fibrinolytic enzymes, including urokinase, tissue plasminogen activator (t-PA) and plasmin. It had no "Kringle structure" seen in ordinary fibrinolytic enzyme molecul … More es. NK antibodies prepared in rabbits failed to respond to proteases (subtilisin) thus far isolated from Bacillus subtilis by Ouchterlony and ELISA methods.
By WBCLT method, the poly-Glu rich fraction of natto was found to potentiate the plasma fibrinolysis. A crude material, containing 20,000 NK units and 113 mg/g poly-Glu, prepared from a high-NK natto product, was fed to 15 male Wister rats at 48,000 NK units/kg b.w. daily for 3 months. After dosing or in comparison with the non-dosed group, plasma ELT shortened significantly (p<0.01),and plasma amidolysis (pyro-Glu-Gly-Arg-pNA and H-D-Val-Leu-Lys-pNA,p<0.01) increased. Renal and pulmonary PA activities (p<0.1) increased, whereas APTT and Re-Ca^<++>T unchanged. The enteric coated capsules of NK (2.13 CU/mg of protein) were prepared. EFA was gradually increased from the 1st day to the 8th day after administration. FDP was statistically higher (p<0.001) on the 1st day of NK administration in comparison with pre-administration. The significant increase of t-PA antigen (p<0.005) was also proved over the long te rm (Clinical applications of NK capsule are now in progress under the permission of University Committee for Ethics).
These results demonstrate that NK or NK-rich natto will be useful not only for the treatment of embolism but also for prevention of the disease,since they are safety and also canbemass-produced. Less

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Hiroyuki SUMI: "Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase." Acta Haematologica. 84. 139-148 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroyuki SUMI: "Effect of various pdyamino acids and D-and L-amino acids on the blood fibrinolytic system." Comparative Biochemistry and Physiology. 102B. 159-162 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "納豆キナーゼと線溶系" 化学と生物. 29. 119-123 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "血栓溶解酵素ナットウキナーゼの活性測定法" 日本醸造協会誌. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "血栓性疾患に対する経口線溶療法" Molecular Medicine. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "血栓溶解酵素ナットウキナーゼ" 学術月報. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "機能性食品素材マニュアル" CMC(東京), 360 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須見 洋行: "醗酵飲食品の開発と機能性" 先端科学技術・情報教育センター(東京), 210 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIROYUKI SUMI: "Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase." Acta Haematologica. 84. 139-148 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIROYUKI SUMI: "Effect of various poly amino acids and D- and L-amino acids on the blood fibrinolyt.c system." Comparative Biochemistry and Physiology. 102B. 159-162 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIROYUKI SUMI: "Nattokinase and fibrinolysis." Kagakuto-Seibutsu. 29. 119-123 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIROYUKI SUMI: "Deteimination method of Hirombolytic enzyme nattokinase." Journal of the Brewing Society of Japan. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIROYUKI SUMI: "Oral fibrinolytic therapy for thrombolytic diseases." Molecular Medicine. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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