1992 Fiscal Year Final Research Report Summary
Expression of BMP family proteins in mammalian cells and production of monoclonal antibodies
| Project/Area Number |
02558017
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
代謝生物化学
|
|---|
| Research Institution | University of Tsukuba, Institute of Applied Biochemistry |
|---|
Principal Investigator |
UENO Naoto University of Tsukuba, Institute of Applied Biochemistry, Assistant professor, 応用生物化学系, 講師 (40221105)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WAKIMATSU Mitsuhiro Takeda Chemical Industries Inc., Senior research investigator, 筑波研究所(現開拓研究所), 主任研究員
|
|---|
| Project Period (FY) |
1990 – 1992
|
| Keywords | BMPs / bone formation / cartilage formation / TGF-beta / osteoporosis |
|---|
| Research Abstract |
We have screened a genomic DNA library of Xenopus laevis with rat activin betaA gene as a probe. We have isolated 5 independent genes and one of the genes was found to be encoding a protein closely related to mammalian activin. Interestingly, the rest of the gene contained an amphibian counterpart of mammalian bone morphogenetic proteins, which was purified based on their ability to induce ectopic bone formation in vivo. Eventually, we isolated cDNAs of Xenopus BMP-2,-4 and -7. In order to obtain pure BMP proteins and supply them for experimental and clinical studies, we tried to express BMP proteins in mamalian cells. We have found that expression rate is relatively high(20 ug/L) when BMP-4 cDNA is introduced into CHO cells with pSD(X) expression vector. On the other hand, we successfully raised polyclonal antibodies against BMP-2 peptide and beta-galactosidase/BMP-4 fusion protein. The former antibody (Ab383) was found to react with BMP-4 as well as BMP-2 and the latter (Ab97) was specific to BMP-4. We partially purified the BMP-4 expressed in CHO cells based on its immunoreactivity. The final preparation of the recombinant Xenopus BMP-Xenopus BMP-2 has been shown be indistinguishable from mammalian BMP-2 biochemically ann biologically. Therefore, Xenopus BMPs are thought to be a promising molecule to develop drugs for bone fracture and osteoporosis. In addition, our antibodies are demonstrated to be useful to screen BMPs in the extract of bovine bone.
|
-
-
-
-
-
-
-
-
[Publications] Ueno, N., Shoda, A., Takebayashi, K., Suzuki, A., Nishimatsu, S., Kikuchi, T., Wakimasu, M., Fujino, M. and Murakami, K.: "Identification of bone morphogenetic protein-2 in early Xenopus laevis embryos." Growth Factors. 7. 233-240 (1992)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
