1991 Fiscal Year Final Research Report Summary
Purification of platelet-derived factors that stimulate DNA and proteoglycan syntheses by chondrocytes.
| Project/Area Number |
02558019
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Hiroshima University (1991)
Osaka University (1990) |
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Principal Investigator |
KATO Yukio Hiroshima University, School of Dentistry, Professor, 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
ASADA Akira Osaka University, Faculty of Dentistry, Lecturer, 歯学部, 講師 (50028734)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Chondrocyte / Platelet / Growth factor / Proteoglycan synthesis / DNA synthesis |
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| Research Abstract |
Removal of blood clots at sites of bone fracture or injection of an t i-coagulan t delay s heal ing of bone fracture. This suggests platelets may contain or release factors that stimulate proliferation and differentiation of chondrocytes and bone cells. In the present study. we purified from the extracts of human platelets two polypeptides that stimulated DNA and proteoglycan syntheses in chondfocyte cultures. In SDS-PAGE, they migurated at positions corresponding to Mr. 10000-12000 and 28000-29000. Thesefactors differed from FGF. TGF-beta and PDGF with respects to their biological and physicochemical properties. Thus. the novel growth factors from human platelets will be useful for promoting healing of bone fracture.
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