1991 Fiscal Year Final Research Report Summary
Preparation and Properties of a Micro Enzyme Electrode for Creatine
| Project/Area Number |
02640452
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分析・地球化学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
IKEDA Sanae The University of Tokushima, Technical College, Professor, 工業短期大学部, 教授 (20035801)
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| Co-Investigator(Kenkyū-buntansha) |
MOTONAKA Junko The University of Tokushima, Technical College, Assistant, 工学短期大学部, 助手 (40035811)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Creatine / Enzyme Electrode / Creatine Amidinohydrolase / Sarcosine Oxidase |
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| Research Abstract |
A micro enzyme electrode using creatine amidinohydrolase (EC 3.5.3.3) and sarcosine oxidase (EC 1.5.3.1) was developed for measuring creatine. The sensor consisted of platinum and silver wires fixed in a glass tubing with epoxy resin adhesive, and creatine amidinohydrolase and sarcosine oxidase immobilized on their tip by cross-linking method. The calibration curve for measurement of creatine, the effects of pH temperature, the amount of flavin adenine dinucleotide and concomitant compound on the sensitivity, and the life-time of the sensor were examined. Under optimal conditions, the response of the sensor was linear in the concentration of 10 muM - 0.65 muM creatine.
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