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1992 Fiscal Year Final Research Report Summary

N_2-fixation in marine cyanophyte Trichodesmium cultured under artificial conditions

Research Project

Project/Area Number 02640530
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionTokai University (1992)
Okazaki National Research Institutes (1990-1991)

Principal Investigator

OHKI Kaori  Tokai Univ., Professor School Mar. Sci.Technol., Dept. Mar.Sci, 海洋学部, 教授 (90101104)

Project Period (FY) 1990 – 1992
KeywordsCyanophyte / Trichodesmium / N_2-fixation / Nitrogenase / nif H, D, K / Regulation / Fe-protein / FeMo-protein
Research Abstract

Marine, pelagic cyanophyte, Trichodesmium, able to fix N_2 under aerobic conditions in the light. Mechanisms for the maintenance of N_2-fixation activity in Trichodesmium under aerobic conditions were studied. Results and further view are as follow : 1. N_2-fixation of Trichodesmium is regulated at least two different levels, biosynthesis of nitrogenase and post-translational modification of the Fe-protein of nitrogenase. 2. Examination by immuno-electron microscopy showed that Trichodesmium did not develop the special cells (heterocysts) which were the site of aerobic N_2-fixation in cyanophyte. Almost all cells contain nitrogenase. 3. The high homology of the primary structure of the Fe-protein of nitrogenase between Trichodesmium and other N_2-fixing organisms was found by sequencing of the structure gene (nif H). Sequencing of the structure genes of the FeMo-protein of nitrogenase (nif D,H) is under progress. 4. N_2-fixation activity of Trichodesmium is maintained by the light. Activity was abruptly lost when cells were transferred from light to the dark. The Fe-protein of nitrogenase became inactive form in the dark, and light was required to change from inactive to active form. Protein synthesis inhibitors inhibit the activation process by light. These results suggest that (1)light is required to maintain the nitrogenase activity not only to supply ATP and/or reductant by photosynthesis but also keeping the Fe-protein active form, and that (2)proteinous factor(s) which turns over rapidly acts during the activation process. Determination of proteinous factors(s) and further analysis of the effect of light on transcriptional and translational levels of nitrogenase are required.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] K.Ohki: "Light-dependent maintenance of active nitrogenase in the non-heterocystous cyanophyte Trichodesmium sp.NIBB1067." Progress in Photosynthesis Research (ed.N.Murata),Kluwer Academic Publishers,Dordrecht.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ohki: "Regulation of nitrogenase activity in relation to the light-dark regime in the filamentous non- heterocystous cyanobacterium Trichodesmium sp.NIBB1067." J.Gen.Microbiol.138. 2679-2685 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ohki: "Trichodesmium: Establishment of culture and characteristics of N_2-fixation." Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs.(Eds.Carpenter,E.J., Capone,D.G.and Rueter,J.G.) Kluwer Academic Publishers,Dordrecht.307-318 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.P.Zehr: "Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB1067." J.Bacteriol.173. 7059-7062 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.P.Zehr: "Arrengement of nitrogenase structural genes in an aerobic filamentous non-heterocystous cvanobacterium." J.Bacteriol.173. 7055-7058 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ohki: "Regulation of nitrogen-fixation by different nitrogen sources in the marine non-heterocystous cyanobacterium Trichodesmium. sp. NIBB1067." Arch.Microbiol.156. 335-337 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ohki: "Experimental study of Trichodesmium, a nitrogen-fixing cyanophyte in tropical and subtropical sea areas." Marine Biology. It's accomplishment and feature prospect (Eds.Mauchline,J. and Nemoto,T.) Hokusensha,. 209-219 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.P.Zehr: "Antisira to nitrogenase generated from an amplified DNA fragment from natural populations of Trichodesmium sp." Appl.Environ.Microbiol.56. 3527-3531 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.G.Rueter: "The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of Trichodesmium (cyanophyceae)." J.Phycol.26. 30-36 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ohki: "Light-dependent maintenance of active nitrogenase in the non-heterocystous cyanophyte Trichodesmium sp. NIBB1067" Progress in Photosynthesis Research (ed. N.Murata Kluwer Academic Publishers).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ohki: "Regulation of nitrogenase activity in relation to the light-dark regime in the filamentous non-heterocystous cyanobacterium Trichodesmium sp. NIBB1067" J.Gen.Microbiol. 138. 2679-85 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ohki: "Trichodesmium : Establishment of culture and characteristics of N_2-fixation. (1992)" Marine Pelagic Cyanobacteria : Trichodesmium and Other Diazotrophs. (eds. Carpenter et al. Kluwer Academic Publishers). 307-318 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.P.Zehr: "Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB1067" J.Bacteriol. 173. 7059-62 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.P..Zehr: "Arrengement of nitrogenase structural genes in an aerobic filamentous non-heterocystous cyanobacterium. (1991)" J.Bacteriol. 173. 7055-58 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ohki: "Regulation of nitrogen-fixation by different nitrogen sources in the marine non-heterocystous cyanobacterium Trichodesmium. sp. NIBB1067" Arch. Microbiol. 156. 335-37 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ohki: "Experimental study of Trichodesmium, a nitrogen-fixing cyanophyte in tropical and subtropical sea areas" Marine Biology. It's accomplishment and feature prospect (Eds. Mauchline & Nemoto, Hokusensha). 209-219 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Zehr: "Antisera to nitrogenase generated from an amplified DNA fragment from natural populations of Trichodesmium sp" Appl.Environ.Microbiol. 56. 3527-31 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.G.Rueter: "The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of Trichodesmium (Cyanophyceae)" J.Phycol. 26. 30-36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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