1992 Fiscal Year Final Research Report Summary
Study on the differentiation of sex organs using female and male cells in heterosporous ferns
| Project/Area Number |
02640538
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物形態・分類学
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| Research Institution | Fukui University |
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Principal Investigator |
MAEDA Masuo Faculty of Education, Fukui Univ., Associated Prof., 教育学部, 助教授 (10020140)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Michiyo Faculty of Education, Fukui Univ., Assistant, 教育学部, 教務職員
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| Project Period (FY) |
1990 – 1992
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| Keywords | ferns / heterospory / sex organ differentiation / cell culture / protoplast / female and male gametophytes / meiosis / microsporocytes |
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| Research Abstract |
(1)Protoplasts isolated enzymatically from juvenile leaflets of sporophytes in fern Lygodium japonicum were inoculated in a MS medium containing NAA 2.7 um, BAP 2.2 um, 0.6 M mannitol, and 0.05 M sucrose. Cell division took place within 8 days of culture with 10-15 cells were observed. About 2 months later, cordate prothallia developed bearing both antheridia and archegonia under hormone-free medium.
(2)Sporophytes in heterosporous fern Salvinia natans All. were aseptically obtained by co-culture of female and male gametophytes derived from two types of spores (megaspores and microspores). Protoplasts were isolated enzymatically from juvenile leaflets of sporophytes. Cell division took place within 6 days of culture.
(3)In vitro multiplication of Marsilea quadrifolia L., a heterospory, was carried out by tissue culture method.
(4)Topoisomerase I was isolated, purified, and studied from microsporocytes of Lilium.
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