• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1991 Fiscal Year Final Research Report Summary

Mesoderm induction and gene expression by activin A (=EDF)

Research Project

Project/Area Number 02640562
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionYokohama City University

Principal Investigator

ASASHIMA Makoto  Dept. of Biology Yokohama City University Professor, 文理学部, 教授 (00090564)

Project Period (FY) 1990 – 1991
Keywordsactivin / mesoderm induction / Xenopus / follistatin / EDF / TGF-beta super family / activin-binding protein / embryonic induction
Research Abstract

Summary. Recently the mesoderm-inducinig effects of the transforming growth factor beta (TGF-beta)family, of proteins have been widely examined. In an attaint to elucidate the functions of these proteins, porcine inhibin A and activin A(erythroid differentiation factor ; EDF)were examined. Treatment of explants with activin A led to differentiation of mesodermal derivatives such as mesenchyme, notochord, blood cells and muscle, but inhibin A had a much lesser effect. The niesodernial differentiation induced by activin A was also comfirmed by analyses using a polyclonal antibody against muscle myosin. By indirect immufluorescence analysis, the differentiation of muscle blocks was observed in the activin-Atreated explants, whereas no differentiation was observed in inhibin-A-treated and control explants. These findings confirm that this protein of the TGF-beta family, has mesoderm-inducing ability.
Activin A, a member of the transforming growth factor beta superremily, has recently been f … More ound to have potent mesoderm-inducing activity on isolated early Xenopus animal-cap cells. We measured the activin activity of the Xenopus egg extract by using an erythroid-differentiating test with Friend leukemia cells. The results showed that an activin homologue is, indeed, contained in unfertilized eggs and blastulae of Xenopus laevis in a considerable amount. This activity was eluted at the same retention time as human activin A when fraction2ted by reversed-phase HPLC. Furthermore, the fraction containing erythroid-differentiating factor activity had mesoderm-inducing activity on Xenopus animal-cap cells. The mesoderm-inducing activity of this traction was suppressed when coincubated with follistatin, an activin-binding protein. These results suggest that an endogenous activin may be a natural mesoderm-inducing factor acting In Xenopus embryogenesis.
The induction of mesoderm is an important process in early amphibian development. In recent studies, activin has become an effective candidate for a natural mesoderm-inducing factor. In the present study, we show that follistatin, an activin-binding protcin puriFied from porcine ovary, inhibits the mesoderm-inducing activity of recombinant human activin A(rh activin A). which is identical to the crythroid differentiation factor(EDF). The quantity of follistatin required for effective suppression of activin was more than three-fold that of activin(w : w). Follistatin also inhibited the mesoderminducing activity of the vegetalizing factor purified from chick embryos, suggesting that the vegetalizing factor is closely related to activin. Less

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Asashima,M.,H.Nakano,H.Uchiyama,T.Nakamura,Y.Eto,D.Ejima,S-I.Nishimatsu,N.Ueno and K.Kinosita: "The presence of activin (Erythroid Differentiation Factor) in Unfertilized eggs and blastulae of Xenopus laevis." Proc.N.A.S.88(15). 6511-6514 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asashima,M.,H.Nakano,H.Uchiyama,H.Sugino,T.Nakamura,Y.Eto,D.Ejima,M.Davids,S.Plessow,I.Cichoka & K.Kinosita: "Follistatin inhibits the mesoderm-inducing activity of activin A and the vegetalizing factor from chicken embryo." Roux's Arch.Dev.Biol.200(1). 4-7 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariizumi,T.,N.Moriya,H.Uchiyama and M.Asashima: "Concentration Dependent Inducing Activity of Activin A." Roux's Arch.Dev.Biol.200. 230-233 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asashima,M.,H.Uchiyama,H.Nakano,Y.Eto,D.Ejima,M.Davids,S.Plessow J.Born,P.Hoppe,H.Tiedemann and H.Tiedemann: "The vegetalizing factor from chicken embryos:Its EDF (activin)-like activity." Mechanism of Cell Development. 34. 135-141 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukui,A.,A.Tashiro,H.Koyama,Y.Iwamura and M.Asashima: "A new cell line (XTY) from a tumor of Xenopus laevis." Experientia. 48. 87-91 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariizumi,T.,K.Sawamura,H.Uchiyama and M.Asashima: "Dose and time-dependent mesoderm induction and outgrowth formation by activin A in Xenopus laevis." Int.J.Develop.Biol.35. 407-414 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] "Mesoderm induction in early amphibian embryos by activin A and its related gene products in Xenopus laevis.In:Frontiers in muscle reserch.eds.E.Ozawa et al." Elsevier Science Pubrishers B.V., 12 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 駒崎 伸二,浅島 誠: "細胞とバイオサイエンス(I);形態形成" 朝倉書店, 129-136 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Asashima, H. Nakano, H. Uchiyama, H. Sugino, T. Nakamura, Y. Eto, D Ejima, S. Nishimatsu, N. Ueno and K. Kinoshita.: "The Presence of Activin (Erythroid Differentiation Factor in Unfertilized Eggs and Blastulae of Xenopus laevis." Proc. Nat. Acad. Sci.88(15). 6511-6514 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima, H. Uchiyama, H. Nakano, Y Eto, D. Ejima, H. Sugino, M. Davids, S. Plessow, J. Born, P. Hoppe, H. Tiedemann and H. Tiedemann.: "The Vegetalizing Factor from chicken embryos : its EDF (activin A)-like activity." Mechanisms of Development. 34. 135-141 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima, H. Nakano, H. Uchiyama, H. Sugino, T. Nakamura, Y. Eto, D. Ejima, M. Davids, S. Plessow, I. Cichoka and K. Kinoshita.: "Follistatin inhibits the mesoderm inducing activity of activin A and the vegetalizing factor from chicken embryo." Roux's Archives Develop. Biol. 200(1). 4-7 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Ariizumi, N. Moriya, H. Uchiyama and M. Asashima: "Concentration Dependent Inducing Activity of Activin A." Roux's Archiv. Develop. Biol. 230-233 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Ariizumi, K. Sawamura, H. Uchiyama and M. Asashima: "Dose and time-dependent mesoderm induction and outgrowth formation by activin A in Xenopus laevis." Int. J. Develop. Biol.35(4). 407-414 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N. Ueno, M. Asashima, S. Nishimatsu, A. Suzuki and K. Murakami: "Mesoderm induction in early amphibian embryos by activin A and its related gene products in Xenopus laevis." Elsevier Science Publishers B. V. Frontiers in muscle research. 17-28 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N. Moriya, Y. Kudo, H. Uchiyama, A. Fukui and M. Asashima: "Xenopus Cell Lines have Inducing Activities on Presumptive Ectoderm of Cynops Embryo." Journal of Yokohama City University. 5, 1-13. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Fukui, A. Tashiro, H. Koyama, Y. Iwamura and M. Asashima: "A new cell line (XTY) from a tumor of Xenopus laevis." Experientia. 48. 87-91 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima, K. Shimada and C. J. Pfeiffer: "Magnetic Shielding Induces Early Developmental Abnormalities in the Newt, Cynops pyrrhogaster." Bioelectromagnetics. 12. 215-224 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima, H. Nakano, K. Shimada, K. Kinoshita, K. Ishii, H. Shibai and N. Ueno: "Mesodermal induction in early amphibian embryos by activin A (erytroid differentiation factor)." Roux's Archives Develop. Biol.198. 330-335 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima, H. Nakano, H. Uchiyama, M. Davids, S. Plessow, B. Loppnow-Blind, P. Hoppe, H. Dau and H. Tiedemann: "The Vegetalizing Factor Belongs to a Family of Mesoderm Inducing Proteins Closely Related to Erythroid Differentiation Factor." Naturwissenschaften. 77. 389-391 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nakano, K. Kinoshita, K. Ishii, H. Shibai and M. Asashima: "Activities of Mesoderm-Inducing Factors Secreted by Mammalian Cells in Culture." Develop. Growth & Differ. 32(2). 165-170 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. J. Pfeiffer, H. Pyle and M. Asashima: "Blood Cell Morphology and Counts in The Japanese Newt (Cynops pyrrhogaster)." Journal of Zoo and Wildlife Medicine. 21(1). 56-64 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. J. Pfeiffer, M. Asashima, C. H. Cho and K. Shimada: "Observations on cardiac ultrastructure of the adult Japanese newt, Cynops pyrrhogaster." J. Submicrosc. Cytol. Pathol.22(1). 17-26 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. J. Pfeiffer, M. Asashima: "Gastric Leiomyomas in the Japanese newt, Cynops pyrrhogaster Ultrastrctural observation." J. Comp. Pathol.102. 79-87 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Asashima: "Embryonic Induction and Cell Differentiation During the Amphibian Development." Zoological Science of Japan of Japan. 7, No. 6. 1015-1016 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi