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1991 Fiscal Year Final Research Report Summary

Distribution and physiological function of atrial and brain natriuretic peptides in lower vertebrates.

Research Project

Project/Area Number 02640587
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionKanagawa Dental College

Principal Investigator

UEMURA Haruko  Kanagawa Dental College, Department of Dentistry, Professor, 歯学部, 教授 (00147985)

Co-Investigator(Kenkyū-buntansha) TEZUKA Yoko  Kanagawa Dental College, Department of Dentistry, Assistant, 歯学部, 助手 (10214313)
NAKAMURA Sumio  Kanagawa Dental College, Department of Dentistry, Lecturer, 歯学部, 講師 (80104496)
HIROHAMA Tohru  Kanagawa Dental College, Department of Dentistry, Lecturer, 歯学部, 講師 (90101211)
Project Period (FY) 1990 – 1991
KeywordsAtrial natriuretic peptide peptide / Brain natriuretic peptide / Endothelin / Intragranular colocalization / Drinking behavior / Phylogeny
Research Abstract

1. Atrial and brain natriuretic peptides (ANP, BNP).
(1) The distribution of binding sites for ANP was studied by whole-body autoradiography in medaka, which was injected [ ^<125>I]-rat ANP intraperitoneally. High levels of radioactivity were found in the gill. ANP may act on the gill and play some roles, such as regulation of water and salt balance in fish. (2) By the double immunogold labeling, it was found that ANP and BNP coexist in the same secretory granules of atrial and ventricular cardiocytes in the representative animals of all vertebrate classes (two species each of teleosts and amphibians, and one species each of reptiles, birds and mammals). In most of animals examined, the immunoreactivity for ANP was stronger than that for BNP, but the opposite was true in the medaka and the soft-shelled turtle. Both peptides may be secreted simultaneously into circulation to play some coordinated functions. (3) Porcine BNP elicited a significant increase in water intake, when administered intraperitoneally (5 and 10 mug/bird) or intracerebroventricularly (0.3 mug/birds) in the water-replete Japanese quail. BNP may act on the brain to stimulatd drinking behavior.
2. Endothelin (ET)
(1) Immunoreactive ET was estimated in plasma of nonmammalian vertebrates (one species each of cyclostomes, elasmobranchs, amphibians, reptiles and birds and two species of teleosts, employing radioimmunoassay for ET-1. Levels of immunoreactive ET-1 were similar to those of human in all animals (mean : 3.3-9.6 pg/ml), except that it was rather low in the hagfish (0.7 pg/ml). These results suggest an endocrine function of the peptide in these species. (2) Immunoreactive ET-1 was found in the hypothalamo-hypophysial system of the representative animals of all vertebrate classes. Further, the double immunogold labeling revealed in rats that immunoreactive ET-1 coexisted with neurohypophysial hormones in the secretory granules of the vasopressinergic and oxytocinergic axons, respectively.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Haruko Uemura: "Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma examined by electron microscopy,immunohistochemistry and radioimmunoassay." Cell and Tissue Research. 260. 235-247 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruko Uemura: "Immunoreactive and bioactive atrial natriuretic peptide in the carp heart." Zoological Science. 8. 885-891 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumio Nakamura: "Atrial natriuretic peptide and brain natriuretic peptide coexist in the secretory granules of human cardiac myocytes." American Journal of Hypertension. 4. 909-912 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoko Kasuya: "Distribution of immunoreactive atrial and brain natriuretic peptides in the geart of the newt,Cynops pyrrhogaster." Acta Histochemica et Cytochemica. 25. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoko Tezuka: "Dipsogenic action of brain natriuretic peptide and endothelin-1 in the Japanese quail,Coturnix coturnix japonica." Zoological Science. 9. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruko Uemura: "Immunoreactive endothelin in plasma of nonmammalian vertebrates." Journal of Cardiovascular Pharmacology. 17(Suppl.7). S414-S416 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小林 英司: "新生理科学大系20巻(分担執筆)" 医学書院, 8 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasegawa,K.,K.Fujii,Y.Kasuya,H.Uemura and H.Kobayashi: "Neuroendocrinology:New Frontiers" Brain Research Promotion,London,Tubingen, 6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uemura, H., M. Naruse, T. Hirohama, S. Nakamura, Y., Kasuya and T. Aoto.: "Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma and radioimmunoassay" Cell Tiss. Res.260. 235-247 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uemura, H., M. Naruse, Y. Takei, S. Nakamura, T. Hirohama, K. Ando and T. Aoto.: "Immunoreactive and bioactive atrial natriuretic peptide in the carp heart" Zoological Science. 8. 885-891 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, S., M. Naruse, K. Naruse, M. Kawana, T. Nishikawa, S. Hosoda, I. Tanaka, T. Yoshimi, I. Yoshihara, T. Inagami, H. Uemura and H. Demura.: "Atrial natriuretic peptide and brain natriuretic peptide coexist in the secretory granules of human cardiac myocytes." American Journal of Hypertension. 4. 909-912 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasuya, Y., T. Hirohama and H. uemura.: "Distribution of immunoreactive atrial and brain natriuretic peptides in the heart of the newt, Cynops phrrhogaster" Acta Histochemica et Cytochemica. 25(3).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tezuka, Y., H. Kobayashi and H. Uemura.: "Dipsogenic action of brain natriuretic peptide and endothelin-1 in the Japanese quail, Coturnix coturnix japonica." Zoological Science. 9(3).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uemura, H., M. Naruse, K. Naruse, T. Hirohama, H. Demura and Y. Kasuya.: "Immunoreactive endothelin in plasma of nonmammalian vertebrates." Journal of Cardiovascular Pharmacology. 17(Suppl. 7). S414-S416 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasuya, Y., H. Kobayashi and H. Uemura.: "Endothelin-like immunoreactivity in the nervous system of invertebrates and fish." Journal of Cardiovascular Pharmacology. 17(Suppl. 7). S463-S466 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, S., M. Naruse, K. Naruse, H. Demura and H. Uemura.: "Immunocytochemical localization of endothelin in cultured bovine endothelial cells." Histochemistry. 94. 475-477 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasuya, Y., H. Kobayashi and H. Uemura.: "The distribution of an immunoreactive endothelin-like substance in the nervous system of the nereid, Neanthes diversicolor (Annelida)." Cell Tiss. Res.261. 485-492 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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