1991 Fiscal Year Final Research Report Summary
On the mechanisms of lectin gene expression in a mushroom, Aleuria aurantia.
| Project/Area Number |
02660080
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
応用生物化学・栄養化学
|
|---|
| Research Institution | Depatment of Horticulture, Chiba University |
|---|
Principal Investigator |
NAGATA Yoshiho Chiba University, Department of Horticulture, Professor, 園芸学部, 教授 (60013322)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Keywords | Aleuria aurantia / Lectin / Primary structrue / Secondary structure / Crystallization |
|---|
| Research Abstract |
1. Nucleotide sequence of Aleuria aurantia lectin(AAL)cDNA.
Complete nucleotide sequence of AAL cDNA was determined. Consequently, it was found that AAL was consisted of 312 amino acid residues, having Pro in N-terminus and Trp in C-terminus. Data of the nucleotide sequence revealed that AAL does not have the signal sequence since there was only one Met residue prior to N-terminal Pro residue.
2. Protein chemical properties of AAL.
The deduced amino- acid sequence shows that AAL- includes six internal homologous regions, and has considerable homology with a hemagglutinin from a Gram-negative bacterium, Myxococcus xanthus. Circular dichroism spectra revealed a very low content of alpha-helical and beta-sheet structures.
3. Crystallization of AAL.
AAL was crystallized and the crystals showed hexagonal bipyramid in shape. Crystallographic parameters were determined by X-ray precession photography.
4. Developmental appearance of AAL during fruiting-body formation
Crude extract prepared from the fruiting-body contained hemagglutinating activity, while that from the mycelia did not. Material that reacted with anti-AAL antibody was also included in the extract of the fruiting-body but not in that of the mycelium. These results sugested that expression of AAL gene is developmental regurated, and related to the formation of the fruiting-body in A. aurantia.
|
-
-
-
-
-
-
[Publications] Fukumori, F., Takeuchi, N., Hagiwara, T., Ohbayashi, H., Endo, T., Kochibe, N., Nagata, Y. and Kobata. A.: "Primary structure of a fucose-specific lectin obtained from a mushroom, Alueria aurantia." J. Biochem.107. 190-196 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nagata, Y., Fukumori, F., Sakai, H., Hagiwara, T., Hiratsuka, Y., Kochibe, N. and Kobata. A.: "Crystallization and characterization of a lectin obtained from a mushroom, Alueria aurantia." Biophys. Biochim. Acta. 1076. 187-190 (1991)
Description
「研究成果報告書概要(欧文)」より
-
-
-
