1991 Fiscal Year Final Research Report Summary
Studies on biosynthesis and reception of gibberellin-related antheridiogens in gametophytes of schizaeaceous ferns
| Project/Area Number |
02660133
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMANE Hisakazu The University of Tokyo Fac. of Agric. Assistant, 農学部, 助手 (80090520)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Schizaeaceae / Anemia phyllitidis / Lygodium japonicum / biosynthesis / antheridiogen / antheridic acid / 3d-hydroxy-9.15-cyclo-GA_9 / 9.15-cyclo-GA_9 |
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| Research Abstract |
[ ^2H_2]9, 15-Cyclo-GA_9 and [ ^2H_2]3alpha-hydroxy-9, 15-cyclo-GA_9 were fed to prothallia of the fern, Anemia phyllitidis. The metabolites from these feeds were subjected to full scan GC/MS to confirm the conversion of [ ^2H_2]9, 15-cyclo-GA_9 into [ ^2H_2]3alpha-hydroxy-9, 15-cyclo-GA_9 and [ ^2H_2]antheridic acid, and of [ ^2H_2]3alpha-hydroxy-9, 15-cyclo-GA_9 into [ ^2H_2]antheridic aced. These results indicated that antheridic acid is biosynthesized from 9, 15-cyclo-GA_9 via 3alpha -hydroxy-9, 15-cyclo-GA_9 in A. phyllitidis. 3alpha -Hydroxy-9, 15-cyclo-GA_9 was shown to be a native antheridiogen. On the other hand, the conversion of [ ^2H_2]9, 15-cyclo-GA_9 into [ ^2H_2]GA_<73> methyl ester(GA_<73>-Me) was not observed. A novel diterpene hydrocarbon as well as ent-kaurene were detected by full scan GC/MS of the methanol extracts from prothallia of A. phyllitidis, A. flexuosa (the main antheridiogen was also antheridic acid) and Lygodium japonicum. The possibility that the novel diterpene is a precursor of antheridic acid and/or GA_<73>-Me is now under investigation. It was also shown that dark-grown protonemata of L. japonicum incorporated GA_4-Me (active in inducing antheridium formation in L. japonicum protonemata at 10^<-10> M) 3 times more than GA_<20>-Me (ca 1/10^<-4> less active than GA_4-Me).
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