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1992 Fiscal Year Final Research Report Summary

Research on isolation and culture of forest tree species

Research Project

Project/Area Number 02660154
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 林学
Research InstitutionThe University of Tokyo

Principal Investigator

IDE Yuji  The Univ. of Tokyo, Fac. of Agric, Assoc. Prof., 農学部(林), 助教授 (90213024)

Project Period (FY) 1990 – 1992
KeywordsProtoplasts / Betula platyphylla / Quercus acutissima / Acacia mangium / Betula grossa / isolation / Colony formation / Callus formation
Research Abstract

In this research project, isolation and culture of protoplasts from forest tree species were investigated.
Tree species belonging to Fagaceae and Betulaceae which are major broad leaved tree species in Japanese forests and Acacia mangium which is most promising tree species for tropical afforestration were used for research materials.
As the results, protoplasts were successfully isolated from Quercus acutissima, Betula platyphylla var japonica, Betula grossa, Betula schmidtii, Zelkova serrata and Acacia mangium.
And successful callus regeneration was induced by the culture of protoplasts isolated from somatic embryos of Quercus acutissima.
By this research, basic components of enzymes solution and protocols for protoplast isolation from woody plants.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 井出 雄二: "試験管内で継代培養されたシラカンバとミズメの葉からの葉肉プロトプラストの単離" 101回日林論. 491-492 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 原口 雅人: "クヌギ不定胚からのプロトプラストの単離" 102回日林論. 383-384 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji IDE: "Isolation and culture of mesophyll protoplasts from in vitro subcultured Japanese white birch" Bull.Tokyo Univ. For.84. 53-58 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji IDE: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures" Bull.Tokyo Univ. For.85. 43-48 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji IDE: "Callus formation from protoplasts isolated from somatic embryos of Quercus acutissima" J.Jpn.For.Soc.74. 109-113 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Ide,: "Isolation of mesophyll protoplasts from in vitro sub-cultured palantlets of Betula platyphylla var. japonica and Betula grossa." Trans. 101th Mtg. Jpn. For. Soc.491-494 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masato Haraguchi: "Protoplast isolation from adventitious embryos of Quercus acutissima." Trans. 102th Mtg. Jpn. For. Soc.383-389 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji Ide,: "Isolation and culture of mesophyll protoplasts from in vitro subcultured Japanese white birch." Bull. Tokyo Univ. For.84. 53-58 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji Ide,: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures." Bull. Tokyo Univ. For.85. 43-48 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji Ide,: "Callus formation from protoplasts isolated from somatic embryos of Qurcus acutissima." J. Jpn. For. Soc.74. 109-113 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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