1991 Fiscal Year Final Research Report Summary
Induction of enzymes by light in the mycelium of wood decay fungi
| Project/Area Number |
02660181
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
林産学
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
SUZUKI Toshikatsu Tokyo Univ. of Agri., Fac. of Agri., Instructor, 農学部, 講師 (80096677)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZUMI Toshiro Tokyo Univ. of Agri., Fac. of Agri., Professor, 農学部, 教授 (70011856)
HIGAKI Miyato Tokyo Univ. of Agri., Fac. of Agri., Professor, 農学部, 教授 (50078143)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Light / Aeration condition / Tyromyces palustris / Coriolus versicolor / Enzyme induction / Mass loss / Hydrolytic enzyme / Phenoloxidase |
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| Research Abstract |
* Effects of light and aeration condition on the activities of hydrolytic enzymes in the T. palustris mycelium
(1) When the fungus incubated in a shaking culture for five days with the culture medium containing 4 % glucose, the activity of extracellular xylanase was stronger under the irradiation of a 10 W fluorescent lamp than under no irradiation, but this activity was not increased when incubated without glucose under light irradiation.
(2) The activities of extracellular and cell wall bound hydrolytic enzymes were influenced by light irradiation and aeration conditions with wood meal culture medium. The activities of exo-typed cellulase and beta -glucosidase were enhanced in the mycelium which was cultivated under light irradiation and good aeration condition.
* Effects of light and aeration condition on wood decay by C. versicolor
(1) The mass losses of wood by the fungus were greater when the culturing was performed under lightshielded conditions and 25 mm diameter glass tube (0.223g/m^3 cotton wool for aeration) condition than that under the irradiation of 4-20 W fluorescent lamps. And it was found that the masses lost about 45 % in 90 days of culturing.
(2) The activities of extracellular and cell wall bound hydrolytic enzymes were stronger under light-shielded condition with wood meal culture medium than under light irradiation. The activity of extracellular laccase was enchanced under light irraditation. But the activity of peroxidase (optimum pH 5.0-5.5) was stronger under light-shield condition than under light irradiation.
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