• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1991 Fiscal Year Final Research Report Summary

Molecular biological studies on the neurovirulence of murine coronavirus

Research Project

Project/Area Number 02660322
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionNational Institute of Neuroscience, NCNP

Principal Investigator

TAGUCHI Fumihiro  National Institute of Neuroscience, NCNP, Chief researcher, 神経研究所, 室長 (30107429)

Co-Investigator(Kenkyū-buntansha) IKEDA Toshio  National Institute of Neuroscience, NCNP, Postdoctral Fellow, 神経センター・神経研究所, 流動研究員
Project Period (FY) 1990 – 1991
KeywordsCoronavirus / Neurovirulence / Spike (S) protein / Recombinant virus / 細胞融合
Research Abstract

A highly neurotropic murine coronavirus JHM strain has been studied as a model of viral agent to cause acute and subacute encephalomyelitis with demyelination. As a viral factor involving in the neurovirulence. the spike(S)protein comprising the projections on the virion surface is considered to be deeply involved. To study the mechanisms by which JHM strain exhibits the neurovirulence, we have done the molecular biological analysis of S protein of variant JHM viruses showing different neurovirulence. We first of all have isolated a highly neurovirulent variant, cl-2, for rats. This variant has been shown to have a larger S protein as compared with other variants with avirulence. We have isolated the cDNA from mRNA3 encoding the larger S protein and sequence analysis has identified that the larger S protein has 141 amino acid clusters which is missing in a small S protein of avirulent viruses. The 141 amino acid cluster may be implicated with high neurovirulence, although the precise role of the amino acids is yet unclear. We further studied on the larger S protein by expressing in mammalian cells with recombinant vaceinia viruses. The S protein expressed showed no difference in molecular size, immunogenicity or antigenicity as compared with the authentic S protein. Furthermore, the expressed S protein exhibited the fusionability in various cultured cells. These results suggest that the S protein expressed by recombinant vaceinia virus is appropriate for the study of biological activities of the S protein. Experiments are now in progress to see the neurovirulence of JHMV using the recombinant vaccinia viruses with large S protein cDNA.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Taguchi,F.,Ikeda,T.,and Shida,H.: "Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV Variant d-2." Journal of general Virology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatsubrara,Y.,Watanabe,R.,and Taguchi,F.: "Neurovirulence of six different murine coronavirus JHMV variants for rats." Virus Research. 20. 45-58 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takase-Yoden,S.,Kikuchi T.,Siddell,S.,and Taguchi,F.: "Localidation of neutralijing epitopes on the S_1 polypeptide of the murine coronavirus peplomer glycoprotein." Viros Research. 18. 99-108 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tagehi,F.,Yoden,S.,and Siddell,S.: "Expression of the spike proteins of murine coronavirus JHMV using baculovirus vectors." Advances of experimental and biological Medicine. 276. 211-215 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taguchi, F., Ikeda, T., Shida, H.: "Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2." Journal of general Virology. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsubara, Y., Watanabe, R., Taguchi, F.: "Neurovirulence of six different murine coronavirus JHMV variants for rats." Virus Research. 20. 45-58 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takase-Yoden, S., Kikuti, T., Siddell, S., Taguchi, F.: "Localization of neutralizing epitopes on SI polypeptide of the murine coronavirus peploner glycoprotein" Virus Research. 18. 99-108 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taguchi, F, Yoden, S., Syddell, S.: "Expression of the spike protein of the murine coronavirus JHMV using baculovirus vectors" Advances of experimental and biological Medicine. 276. 211-215 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi