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1991 Fiscal Year Final Research Report Summary

Immunohistochemical study on the expression and localization of the epidermal growth factor receptor during morphogenesis

Research Project

Project/Area Number 02670007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyoto University

Principal Investigator

SHIOTA Kohei  Kyoto Univ., Fac. Med., Dept. Anat., Professor, 医学部, 教授 (80109529)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Makoto  Kyoto Univ., Dept. Anat., Instructor, 医学部, 助手 (30232341)
MORI Chisato  Kyoto Univ., Dept. Anat., Instructor, 医学部, 助手 (90174375)
Project Period (FY) 1990 – 1991
KeywordsEpidermal growth factor receptor / Immunohoistochemistry / Organogenesis / Secondary palate / Organ culture / Mouse fetus / Human embryo / Liver tumor
Research Abstract

1. Immunohistochemical studies using the anti-human EGF receptor(EGF-R)rabbit antibody revealed that fetal mouse palates show regional heterogeneity and temporal sequence in EGF-R expression during development. On days 12 and 13, the immunoreactive products were predominantly positive on the oral and medial edge epithelia but minimally on the epithelium of the vertical shelf. The EGF-R immunoreactivity was less intense in the posterior palate as compared with the midpalatal region. The palatal mesenchyme also showed regional heterogeneity and temporal sequence in EGF-R expression.
2. Explanted palates of day-12 mouse fetuses were cultured in a chemically-defined medium and the effects of all-trans-retinoic acid(RA)were examined. RA was added to the culture medium at the concentrations 3-300 ng/ml. RA inhibited palatal fusion dependently on its concentration, although palatal shelves of -normal size tonned and made contact at 3-30 ng/ml. Scanning electron microscopic studies showed that … More the medial cells of RA-treated palates failed to undergo programmed cell death which occurs in control palates. The oral epithelium of control palates lost microvilli and became squamous after palatal fusion, but RA-exposed cells remained ciliated and simulated nasal-hke epithelial cells.
During in vitro palatogenesis, both palatal epithelium and mesenchyme showed regional heterogeneity and temporal sequence in EGF-R expression. During normal palatal development, the EGF-R immunoreactivity became less intense in the medial epithelia. However, in retinoic acid-treated palates, the medial epithelia were continuously positive for EGF-R immunoreactivity. In retinoic acid-treated palates, medial cells were continuously covered with microvilli which disappeared in untreated palates. The alteration in EGF-R expression and epithelial cell differentiation may be responsible for the altered differentiation of palatal shelves and retinoic acid-induced cleft palate.
3. The localization of EGF-R in normal human embryos(Carnegie stages 15, 16, and 20)was examined immunohistochemically. Embryonic tissues were fixed in 4% paraformaldehyde, embedded in paraffin, and serialy sectioned. The sections were stained by avidin-biodn-peroxidase complex method(Vectastain elite ABC kit)with polyclonal anti-human EGF-R antibody. The immunoreactive products against EGF-R were positive in the periderm, the epithelium of the digestive and respiratory tracts, the notochord, the epithelium of the Ratlike's pouch, the fetal cortex of the adrenal gland, germ cells in the gonad, the myocardium and cartilaginous tissue. In the myocardium, the immunoreactivity was intense at Carnegie stages 15 and 16, but became weaker by stage 20. The regional heterogeneity of EGF-R expression was observed in the Ratlike's pouch. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Shiota,K.et al.: "Expression of the epidermal growth factor receptor in developing fetal mouse palates:An immunohistochemical study" American Journal of Anatomy. 188. 401-408 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiota,K.et al.: "Development of the fetal mouse palate in suspension organ culture" Acta Anatomica. 137. 59-64 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Diewert,V.M.and Shiota,K.: "Morphological Observations in normal primary palate and cleft lip embryos in the Kyoto Collection" Teratology. 41. 663-677 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minote,H.et al.: "Immunohistochemical study on epidermal growth factor receptor during carcinogenesis in the rat liver" Archiv Japonica Chirurgica.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Higashide,S.-I.et al.: "Exprssion of epidemal growth factor receptor after partial pancreatectromy in adult rats: An immunohistochemical study" Archiv Japonica Chirurgica.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiota, K. et al: "Expression of the epidermal growth factor receptor in developing fetal mouse palates : An immunohistochemical study." American Journal of Anatomy. 188. 401-408 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiota, K. et al: "Development of the fetal mouse palate in suspension organ culture" Acta Anatomica. 137. 59-64 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Diewert, V. M. and Shiota, K.: "Morphological observations in normal primary palte and cleft lip embryos in the Kyoto Collection." Teratology. 41. 663-677 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minote, H. et al: "Immunohistochemical study on epidermal growth factor receptor during carchinogenesis in the rat liver" Archiv Japonica Chirurgica.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higashide, S. -I. et al: "Expression of epidermal growth factor receptor after partial pancreatectomy in adult rats : an immunohistochemical study" Archiv Japonica Chirurgica.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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