1991 Fiscal Year Final Research Report Summary
Molecular Immunological Analyses of Recognition to T cell For Major and Minor Histocompatibility Antigens
| Project/Area Number |
02670198
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Institute of Medical Science, University of Tokyo |
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Principal Investigator |
TAKIGUCHI Masafumi Institute of Medical Science University of Tokyo, Dept. Tumor Biology Assistant Professor, 医科学研究所, 助手 (00183450)
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| Co-Investigator(Kenkyū-buntansha) |
KARIYONE Ai Institute of Medical Science, University of Tokyo Dept. Immunology Research Asso, 医科学研究所, 教務職員 (50114450)
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| Project Period (FY) |
1990 – 1991
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| Keywords | alloantigen / major histocomoptibility antigen / minor histocompatibility antigen / T cell clone / antigen recognition / HLA antigen / rejection |
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| Research Abstract |
1)Recognition of HLA class I alloantigen by CTL clones.
We investigated the effects of single amino acid substitution of HLA-B35 antigen on recognition of HLA-B35 specific CTL clones. The results demonstrated that recognition of each CTL clones are affected by different amino acid substitutions, indicating that there is a large repertoire of alloreactive T cells for a given HLA class I antigen. Our studies support the concepts that multiple determinants are formed by the binding of various allopeptides to the HLA class I molecules.
2)Recognition of human minor histocompatibility(hmH)antigen by T cell clones.
We investigated the effects of single amino acid substitutions of HLA-B35 antigen on recognition of hmH antigen by hmH specific T cell clones. The substitutions at four residues which are peptide binding sites affects recognition of hmH specific T cell clones, indicating that hmH antigens are recognized as peptides. In fact, we succeeded in isolating the hmH peptide from donor derived B cell lines but not from patient's derived B cell lines.
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