1992 Fiscal Year Final Research Report Summary

Proliferation of double negative (DN) gammadelta T cells in reseponse to allogeneic MHC antigen.

Research Project

Project/Area Number	02670206
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Immunology
Research Institution	Nagasaki University
Principal Investigator	NAKAYAMA Eiichi Nagasaki University School of Medicine, Associate Professor., 医学部, 助教授 (60180428)
Co-Investigator(Kenkyū-buntansha)	MIENO Masahiro Nagasaki University School of Medicine, Assistant Professor., 医学部, 助手 (60039576)
Project Period (FY)	1990 – 1991
Keywords	T-cells / Double negative / gammadelta
Research Abstract	1. In TL transgenic mouse strain C3H.Tg.Tla^a-3.1, gammadelta T cells start to increase from three weeks after birth and reach to about 30% of total T cells in peripheral lymphoid tisene. It was demonstrated that gammadelta T cells in these mice respond to proliferate in stimulation with immobilized anti-TCR delta mAb. 2. Spleen cells from C3H.Tg.Tla^a-3.1 mice proliferated in response to mitomycin C (MMC) treated BALB/c stimulator cells. In these cultures, proliferative response was observed with gammadelta as well as alphabeta T cells. However, when alphabeta T cells were eliminated before MLC, no prolifenation was observed with gammadelta T cells. Thus, proliferative response of gammadelta T cells was dependent on alphabeta T cells. gammadelta T cells recovered after MLR culture showed no cytotoxicity against ^<51>Cr-labeled BALB/c target cells.