1991 Fiscal Year Final Research Report Summary
Regulation of Human T cell and macrophage functions by interleukin 4
| Project/Area Number |
02670211
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Hiroshima University (1991)
University of Occupational and Environmental Health, Japan (1990) |
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Principal Investigator |
YAMASHITA Uki Hiroshima University, School of Medicine, Professor, 医学部, 教授 (00028680)
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| Co-Investigator(Kenkyū-buntansha) |
NISHINO Hideaki University of Occupational and Environment Health, School of Medicine, Research, 医学部, 助手 (40198487)
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| Project Period (FY) |
1990 – 1991
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| Keywords | IL4 / Kiler T cell / Macrophage / IL1 / IL2 / IL2 receptor / LAK / Fc receptor |
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| Research Abstract |
The effects of interleukin 4 (IL 4) on human T cell and macrophage functions were studied in vitro and the following results were obtained.
(1) The in vitro culture of human peripheral blood lymphocyted with allogeneic lymphocytes induced alloantigen-specific killer T cells. Howerer, the addition of recombinant IL 4 suppressed the induction of killer T cell in a dose-dependent manner.
(2) IL 4 did not suppress the lymphocyte proliferative response induced with alloantigen and mitogen stimulation.
(3) IL 4 worked suppressively on the early step of killer T cell induction but not on the late step and did not suppress the effector phase of killer T cells.
(4) IL 4 suppressed the IL 1 production by macrophages.
(5) IL 4 suppressed the IL 2 production and the IL 2 receptor extression by T cells.
(6) IL 4 also suppressed lymphokine activated killer (LAK) cells induced by the culture with IL 2.
(7) IL 4 enhanced the proliferative responses of monocytes and the exptession of MHC class II antigens.
(8) IL 4 suppressed the differentiation of M 1 and HL 60 cell lines into macrophages induced by LPS, IL 1 and TNF.
(9) IL 4 enhanced the growth of M 1 and HL 60 cell lines.
(10) IL 4 suppressed the bone resorbing activity of mouse fetal bones induced by IL 1.
All these results suggest that IL 4 has suppressive activity on T cell and macrophage functions.
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[Publications] Tanaka, Y., Yamashita, U., Watanabe, K., Nagata, K., Mori, N., Oda, S. and Eto, S.: "Calcium dependency of the production of IL 1 and the expression of IL 1 receptors of adult T cell leukemia cells in vitro." Cancer Research. 50. 4344-4348 (1990)
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[Publications] Watanabe, K., Tanaka, Y., Morimoto, I., Yahata, K., Zeki, K., Fujihira, T., Yamashita, U. and Eto, S: "Interleukin 4 as a potent inhibitor of bone resorption." Biochemical and Biophysical Research Communication. 172. 1035-1041 (1990)
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[Publications] Inamoto, Y., Okuno, F., Saito, K., Tanaka, Y., Watanabe, K., Morimoto, I., Yamashita, U. and Eto, S.: "Effect of hyperbaric oxygenation on macrophage functions in mice." Biochemical and Biophysical Research Communication. 179. 886-891 (1991)
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