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1991 Fiscal Year Final Research Report Summary

Abnormality of signal Transduction via T-cell receptors mediated by retrovirus infection

Research Project

Project/Area Number 02670283
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionEhime University

Principal Investigator

YASUKAWA Masaki  Ehime University School of Medicine, Associate Professor, 医学部, 助教授 (60127917)

Co-Investigator(Kenkyū-buntansha) INATSUKI Akira  Ehime University School of Medicine, Senior Resident, 医学部附属病院, 医員
HORIUCHI Takahiko  Ehime University School of Medicine, Assistant Professor, 医学部, 助手 (90219212)
Project Period (FY) 1990 – 1991
KeywordsImmunodeficiency / Retrovirus / T-cell receptor / Cytotoxicity / signal transduction
Research Abstract

In order to clarify the mechanism of immunodeficiency mediated by human retrovirus infection, we investigated the functional alterations of T cells following infection with human T lymphotropic virus type I (HTLV-I), which is the causative agent of adult T-cell leukemia. Herpes simplex virus-specific CD4+ and CD8+ human cytotoxic T-cell (CTL) clones and T-cell receptor-gammadelta + T-cell clones were established. These clones were infected with HTLV-I by co-culture with a HTLV-I-producing T cell line. During the continuous culture of HTLV-I-infected T-cell clones, two distinct phases were observed in terms of cytotoxic activity and expression of the CD3-TCR complex. Early after HTLV-I infection, CTL lost their cytotoxic activity, but this was restored by the addition of lectin. At this time, no differences were observed in the expression of various surface molecules between HTLV-I-infected and uninfected parent cells, except for increased expression of CD25 on HTLV-I-infected cells. On the other hand, late after HTLV-I infection, the cytotoxicity, of HTLV-I-infected cells was almost completely lost, even in the presence of lectin, and expression of the CD3-TCR complex on the cell surface was markedly decreased. Concomitant with the decreased expression of CD3-TCR complex, a decrease in the elevation of cytoplasmic Ca2+ concentration induced by anti-CD3 and anti-TCR monoclonal antibodies was also observed. Our present findings thus show that T-cell functions are profoundly affected by HTLV-I infection through at least two distinct phases.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Akira Inatsuki: "The effect of human T cell leukaemia virus type I infection on a herpes simplex virus-specific CD8+ cytotoxic T cell clone" British Journal of Haematology. 77. 311-314 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaki Yasukawa: "Functional heterogeneity among herpes simplex virus-specific human CD4+ T cells" Journal of Immunology. 146. 1341-1347 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaki Yasukawa: "Proliferation of double-negative (CD4-CD8-) T cells bearing T-cell receptor- in a haemophiliac with human immunodeficiency virus type 1 infection and factor VIII inhibitor" British Journal of Haematology. 79. 372-376 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaki Yasukawa: "Human T-cell leukemia virus type I (HTLV-I) infection of T cells bearing T-cell receptor-:effects of HTLV-I infection on cytotoxicity" International Journal of Cancer. 50. 431-437 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Inatsuki, Masaki Yasukawa, and Yuzuru Kobayashi: "The effects of human T cell leukaemia virus type I infection on a herpes simplex virus-specific CD8+ cytotoxic T cell clone." British Journal of Haematology. 77. 311-314 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaki Yasukawa, Akira Inatsuki, Takahiko Horiuchi, and Yuzuru Kobayashi: "Functional heterogeneity among herpes simplex virus-specific human CD4+ T cells." Journal of Immunology. 146. 1341-1347 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaki Yasukawa, Takaaki Hato, Mitsuru Matsumoto, Kiyonori Takada, Shigeru Fujita, and Yuzuru Kobayashi: "Proliferation of double-negative (CD4-CD8-) T cells bearing T-cell receptor-alphabeta in a haemophiliac with human immunodeficiency virus type 1 infection and factor VIII inhibitor : functional properties of double-negative T-cell receptor-alphabeta + T Cells." British Journal of Haematology. 79. 372-376 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaki Yasukawa, Akira Inatsuki, Yoshihiro Yakushijin, and Yuzuru Kobayashi: "Human T-cell leukemia virus type I (HTLV-I) infection of T cells bearing T-cell receptor gammadelta : effects of HTLV-I infection on cytotoxicity." International Journal of Cancer. 50. 431-437 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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