1992 Fiscal Year Final Research Report Summary
IgA specific helper cytokines in IgA nephropathy
| Project/Area Number |
02670289
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内科学一般
|
|---|
| Research Institution | TOKAI UNIVERSITY |
|---|
Principal Investigator |
SAKAI Hideto Tokai University, Dept.of Medicine, Professor of Medicine, 医学部・内科, 教授 (80102846)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAGAME Mitsunori Tokai Univ.Dept.of Medicine Instructor of Medicine, 医学部・内科, 助手 (10210212)
MIURA Masahiko Tokai University, Dept.of Medicine, Assistant Professor of Medicine, 医学部・内科, 講師 (00190596)
|
|---|
| Project Period (FY) |
1990 – 1992
|
| Keywords | IgA nephropathy / Hyperproduction of IgA / Switch T cell / IL-4 / IL-5 / TGF-beta / IL-6 |
|---|
| Research Abstract |
It is well known that IgA nephropathy is mediated by IgA-dominant immune complexes although the pathogenesis for inducing such immune complexes is unknown.
The aim of the present study was to elucidate cytokines which are responsible for increased production of IgA observed in patients with IgA nephropathy and in some of their relatives. Fifty six patients with IgA nephropathy and 96 family members were studies. Fifty four healthy adults were served as controls. Peripheral blood mononuclear cells (PBMC) were separated and B-cell rich fraction as well as Talpha4 cells (i.e.IgA specific helper T cells) were concentrted by immune absorption technique. Interleukin 4, 5 aad 6 (IL-4, IL-5, IL-6) and tissue growth factor (TGF-beta) were cocultured with these B and T cell cultures alone or in combined forms. IgG, IgA, and IgM-bearing cells in these cultures were quantitated by flow cytometry. Concentration of IgG, IgA, IgM and IgE were measured by ELISA.
There was no significant increase of IgA production in cultures with IL-5, IL-6 and TGF-beta. However, IL-4 induced significant increase in IgA-bearing cells and supernatant IgA in cultures with this cytokine with and without addition of IL-6.
IL-6 did not induce IgA-specific helper activity when added to lymphocyte cultures alone but significantly enhanced IgA production when added with IL-4. It is concluded that IL-4 is the cytokine which specifically induce IgA in humans while IL-5 and TGF-beta show such activity in mice. Further sutdies are warranted to explore clinical significance of IL-4 and IL-6 in patients with IgA nephropathy.
|
Research Products
(16 results)
-
[Publications] Miyazaki,M.,Endoh,M.,Suga,T.,Yano,N.,Kuramoto,T.,Matsumoto,Y,Eguchi,K,Yagame,M.,Miura,M.,Nomoto,Y.and Sakai,H.: "Rheumatoid factors and glomernlonephritis" Clin.Exp.Immunol.81. 250-255 (1990)
Description
「研究成果報告書概要(和文)」より
-
-
[Publications] Yagame,M.,Suzuki,D.,Watanabe K.,Nakao,E,Eguchi,K,Miyazaki M.,Matsumoto,Y.,Yano N.Kuramoto T.,Sako,H.,Ashitate,M.and Suzuki,K: "Significance of levels of circulating IgA-class immune complexes in discriminont onalysis of patients with IgA nepmopathy before cenel biopsy" Nephron. 57. 433-438 (1991)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Yano,Y.,Miyazaki M.,Endoh,M.,Kuramoto,T.,Eguchi,K.,Yagame M.,Nomoto,Y.and Sakai,H.: "Incresse of CD-23 positive cells in peripheral blood foom patients with IgA niplmopathy and non-IgA prolifemtive glomernlonephritis" Nephron. 60. 404-410 (1992)
Description
「研究成果報告書概要(和文)」より
-
-
-
-
-
[Publications] Miyazaki,M., Endoh,M., Suga,T., Yano,N., Kuramoto,T., Matsumoto,Y., Eguchi,K.,Yagame,M., Miura,M., Nomoto,Y., and Sakai,H.: "Rheumatoid factors and glomerulonaphritis" Clin. Exp. Immunol. 81. 250-255 (1990)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Yagame,M., Suzuki,D., Watanabe,K., Eguchi,K., Miyazaki,M., Matsumoto,Y., Yano,N., Kuramoto,T., Sakai,H., Ashitate,M. and Suzuki,K.: "Significance of levels of circulating IgA-class immune complexes in discrieminant analysis of patients with IgA nephropathy before renal biopsy" Nephron. 57. 433-438 (1991)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yano,N., Miyazaki,M., Endoh,M., Kuramoto,T., Eguchi,K., Yagame,M., Nomoto,Y.and Sakai,H: "Increase of CD-23 positive cells in peripheral biood from patients with IgA nephropathy and non-IgA proliferative glomerulonephritis" Nephron. 60. 404-410 (1992)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
