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1992 Fiscal Year Final Research Report Summary

Roles of cellular signal transduction in the defensive mechanism of rabbit stomach

Research Project

Project/Area Number 02670295
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionTokyo University

Principal Investigator

OTA Shinichi  Tokyo University, 2nd Dept, Internal Medicine,, 医学部(病), 助手 (30185269)

Co-Investigator(Kenkyū-buntansha) TERANO Akira  Tokyo University, 2nd Dept, Internal Medicine, associate professor, 医学部(病), 講師 (50155470)
Project Period (FY) 1990 – 1992
KeywordsRabbit gastric mucous cells / Primary culture / mucus secretion / Ca^<2+> / CAMP / ATP / prostaglandin / Defense mechanism
Research Abstract

We investigated the roles of cellular signal transduction system in the defensive mechanism of rabbit somach using primary culture gastric mucous cells. Rabbit gastric epithelial cells were isolated by enzyme digestion and cultured in F-12 medium with 10% FBS. Cultured cells were mainly composed of mucous cells, as assessed by electronmicroscopy and PAS staining. These cells produced prostaglandin (PG)E_2. Influx of extracellular Ca^<2+> and activation of protein kinase C induced stimulation of PGE_2 release, while modulation of cAMP had no effect. Acid secretagogues such as histamine, carhachol, or gastrin did not alter PGE_2 release, while deoxycholic acid markedly increased PGE_2 release mainly through the influx of extracellular Ca^<2+>. Mucus secretion was also stimulated by the increase of intracellular Ca^<2+>, but not by cAMP. ATP, purinergic P2 receptor agonist increased cellular Ca^- and stimulated mucus secretion and PGE_2 release.
In conclusion, intracellular Ca^<2+> might be an important factor in the activation of rabbit gastric defensive mechanism.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Shinichi OTA,et al: "A monolayer culture of gerstric mucous cells from adult rabbits" Gastroenterologia Japonica. 25. 1-7 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi OTA,et al: "Protective effect of tauroursodeoxycholate against chenodeoxychdate-induced damage" Digestive Disenses & Sciences. 36. 409-416 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi OTA,et al: "The effects of acid secretayogues on protective agents of cells form adult rabbits in vitro" American Jounal of Clinical Gerstroanterology. 14(Su. 156- 161 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi OTA,et al: "Roles of Ca2+ and proteinkinase C in the requlation of Prostaglandin E2 release by cultured rabbit gertric cells" Digestive Diseased & Sciences.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.HATA,et al: "Stimulation of prostaglandin E2 relaese from cultured rabbit gerstric cells by sodim deoxycheltb through the in lux of extra cellum Ca2+" Gastro enterology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi Ota, Akira terano, Hideyuki Hiraishi, Hiroyuki Mutoh, Ryo Nakada, Yasuo Hata, Junji Shiga, Tsuneaki Sugimoto: "A monolayer culture of gastric mucous cells from adult rabbits." Gastroenterologia Japonica. 25. 1-7 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinichi Ota, Hiroaki Tsukahara, Akira Terano, Hideyuki Hiraishi, Hiroyuki Mutoh, Yasuo Hata, Tsuneaki Sugimoto: "Protective effect of tauroursodeoxy cholate against chenodeoxycholate-induced damage to cultured rabbit gastric cells." Dig Dis Sci. 36. 409-416 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Ota, Y. Hata, H. Hiraishi, H. Mutoh, A. Terano, T. Sugimoto: "The effects of acid secretagogues on protective agents of gastric cells from adult rabbits in vitro." Am J Clin gastroenterol. 14(Suppl.1). S156-S161 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinichi Ota, Yasuo Hata, Akira Terano, Hideyuki Hiraishi, Hiroyuki Mutoh, Tsuneaki Sugimoto: "Roles of Ca^<++> and protein kinase C in the regulation of prostaglandin E_2 release by cultured rabbit gastric epithelial cells." Dig Dis Sci.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Hata, S. Ota, A. Terano, O. Kohmoto, K. Yoshiura, K. Okano, T. Sugimoto: "Stimulation of prostaglandin E2 release from cultured rabbit gastric cells by sodium deoxyxholate through the influx of extracellular Ca^<2+>." Gastro enterology.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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