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1991 Fiscal Year Final Research Report Summary

Effect of Humoral Factors on the Induction and Expression of Superoxide Dismutases during Development

Research Project

Project/Area Number 02670431
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionYamanashi Medical College

Principal Investigator

ASAYAMA Kohtaro  Yamanashi Medical College, Pediatrics, Fellow, 医学部, 助手 (70129310)

Co-Investigator(Kenkyū-buntansha) UCHIDA Norihiko  Yamanashi Medical College, Pediatrics, Associate Fellow, 医学部, 医員
HAYASHIBE Hidemasa  Yamanashi Medical College, Pediatrics, Associate Fellow, 医学部, 医員
Project Period (FY) 1990 – 1991
KeywordsFree Radicals / Superoxide / Superoxide Dismutases / Fetal Development / Hormone / Active Oxygen Species / Immunohistochemical Staining / Radioimmunoassay
Research Abstract

We investigated developmental profile of copper-zinc and manganese superoxide dismutases (CuZnSOD and MnSOD) in rat fetuses. At the 12th fetal day, both SODs had already been expressed in cardiomyocytes, but not in other tissues. Most organs acquired SODs soon after the period of organogenesis. There was a general trend of richness of both SODs in the epithelial linings and metabolically active sites, although differential distribution between the two SODs also existed. Most tissues accumulated SODs during late gestation, whereas breathing atmospheric oxygen during early extrauterine life did not appreciably intensify the expression of SODs. We also investigated human sutopsy lungs obtained from fetuses and newborns with various gestational ages. Both SODs appeared soon after the development of the epithelia of bronchiles and alveoli. Type II pneumocytes were rich in MnSOD. Thus, MnSOD content was increased in the lungs with bronchpulmonary dysplasia which was associated with hyperplas … More ia of type II pneumovytes.
Dexamethasone (DEX) administered before delivery to the mother is known to protect against hyperoxic lung injury to the premature newborns, by virtue of accelerating maturation of their lung antioxidant enzymes. We investigated the effect prenatal exposure to exogenously administered DEX on the SODs of the fetal lungs and kidneys. Maternal DEX accelerated the maturation of SODs in the fetal lung and kidneys, suggesting significant modification of the expression of SOD by the hormone. This effect was dependent on the dose and timing of the DEX administration.
We studied antioxidant mechanism of human serum. The numerous antioxidants contained in serum are classified either as preventive antioxidants or chain-breaking antioxidants. The former was assayed by the inhibitory activity of autoxidation of phospholipids in brain homogenate, and was found to be higher in children than in adults. The activity was decreased in children with either insulin-dependent diaetes mellitus or chronic renal failure. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Asayama K: "Immunohistochemical localization of copper zine and manganese superoxide disimutases in murine tissues." J.Active Oxygen & Free Radicals. 2. 122-129 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asayama K: "peroxisomal oxidases in vavious tissues of diahetic rats." Dinbetes Res Clin Practice. 11. 89-94 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dobashi K: "Effect of diabetes mellitus induced by streptozotocin on rencl superoxide dismutases in rat." Virchow Archiv B (Cell Pathology). 60. 67-72 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asayama K: "Immunohisto chemical study on peninatal development of rat superoxide dismutases in lung and kidney." Pediatr Res. 29. 487-491 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asayama K: "Concentration of lipid peroxide in serum lipoproteins of insulin-dependent diabetic children." Acta Pediatr.Jpn. 33. 369-374 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内田 則彦: "ヒト血清中抗酸化能の検討・第一編、測定系の検討および小児と成人の比較" 日本小児科学会雑誌. 95. 1944-1950 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asayama K: "High-density lipoprotein (HDL), HDL2, and HDL3 cholesterol concentrations determined in serum of newborns, infants, children, adolescents and adults by use of a micromethod for combined precipitation, ultracentrifugation." Clin Chem. 36-1. 129-131 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokota S: "Peroxisomes of the rat cardiac and soleus muscles increase after starvation." Histochemistry. 93-2. 287-293 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama k: "Oxidative muscular injury and its relevance to hyperthyroidism." Free Radic Bilo Med. 8-3. 293-303 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashibe H: "Prenatal development of antioxidant enzymes in rat lung, Kidney and heart : Marked increase in immunoreactive superoxide dismutases, glutathione peroxidase and ctalase in the kidney." Pediatr Res. 27-5. 472-475 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchida N: "Antioxidant enzymes and lipoperoxide in blood in patients with Kawasaki disease." Acta Pediatr Jpn. 32-3. 242-248 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Antioxidant enzymes and lipoperoxide in blood in uremic patients." Free. Radic. Biol. Med. 9-2. 105-109 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Munim A: "Immunohistochemical localization of copper-zinc and manganese superoxide dismutases in human tissues." Yamanashi Med J. 5-4. 181-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Differential development of antioxidant enzymes in liver of female and castrated and non-castrated male rats." J Clin Biochem Nutr. 9-2. 87-92 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Immunohistochemical localization of copper zinc and manganese superoxide dismutases in murine tissues." J Active Oxygen & Free Radicals. 2-1. 122-129 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Peroxisomal oxidases in various tissues of diabetic tats." Diabetes. Res. Clin. Practice. 11-1. 89-94 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dobashi K: "Effect of diabetes mellitus induced by streptozotocin on renal superoxide dismutases in rat : immunoreactivity and histochemical study." Virchow Archiv B. 60-1. 67-72 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Peroxisomal beta-oxidation in liver and muscles of gold-thioglucose-induced obese mice : correlation with body weight." Int J Obes. 15-1. 45-49 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Immunohistochemical study on perinatal development of rat superoxide dismutases in ling and kidney." Pediatr. Res.29-5. 487-491 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Concentration of lipid peroxide in serum lipoproteins of insulin-dependent diabetic children." Acta Pediatr Jpn. 33-2. 369-374 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asayama K: "Effect of dexamethasone on antioxidant enzymes in fetal rat lung and kidneys." Biol Neonate. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dhaunsi GS: "Demonstration of Cu-Zn superoxide dismutase in rat liver peroxisomes : Biochemical and immunochemical evidense." J Biol Chem. (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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