1991 Fiscal Year Final Research Report Summary
Experimental Study on Pathogenesis of Congenital Hydrocephalus Caused by Myxoviruses
| Project/Area Number |
02670435
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SHIMADA Morimi Shiga University of Medical Science, Department of Pediatrics, Professor., 医学部, 教授 (00079873)
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| Co-Investigator(Kenkyū-buntansha) |
TAKANO Tomoyuki Shiga University of Medical Science, Department of Pediatrics, Junior lecturer, 医学部, 助手 (80236249)
OHNO Masaki Shiga University of Medical Science, Department of Pediatrics, Junior lecturer, 医学部, 助手 (50194254)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Congenital hydrocephalus / Mumps virus / Parainfliuenza virus / Hamster |
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| Research Abstract |
In this experiment, possible brain pathology of suckling hamsters, who had mumps virus or parainfluenza virus type 3 infection during their fetal or early postnatal period, was studied histologically and histochemically.
1. Two-day, 10-day and 30-day-old suckling hamsters were inoculated intracerebrally with 2TCD_<50>(10 ul) of mumps virus. Subsequently all animals developed the mild or sever hydrocephauls. However, hydrocephalus was more pronounced in those animals inoculated earlier.
2. The first pathological sign was noticed on the third day of inoculation as the infiltration of inflammatory cells on the ependymal cell layer. Later on 5 days of inoculation, mild ventricular dilatation become apparent. The aqueductal canal became stenotic after 10 days of inoculation and the dilatation of the lateral ventricles and 3rd ventricle developed rapidly. The neurosis and denuding of the ependymal cell surrounding the ventricular system were followed.
3. In the histochemical examination on virus, mumps virus antigen was detected partly on the choroid plexus and ependymal cells surrounding the ventricular system. All ependymal cells became antigen positive on the 4th day of inoculation.
4. Intraperitoneal inoculation of parainfluenza virus type 3 also caused hydrocephalus in the same pathological processes. Incidence of hydrocephalus was, however, less frequent than that after mumps virus inoculation.
5. Intravenous inoculation of parainfluenza virus to the pregnant hamster could cause hydrocephalus to their offsprings by the transplacental infection.
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Research Products
(13 results)
