• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Process and characterization of LDL modified to be incorporated into macrophages

Research Project

Project/Area Number 02670481
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionKochi Medical School

Principal Investigator

KODAMA Hajime  Kochi Medical School, Department of Dermatology, Professor., 医学部, 教授 (00089905)

Co-Investigator(Kenkyū-buntansha) IKEDA Mitsunori  Kochi Medical School, Department of Dermatology, Research Associate., 医学部, 助手 (70212785)
HIRATA Yasuhiko  Kochi Medical School, Department of Dermatology, Research Associate., 医学部, 助手 (50238367)
OHKAWA Kozo  Kochi Medical School, Department of Dermatology, Research Associate., 医学部, 助手 (80168872)
Project Period (FY) 1990 – 1992
KeywordsXanthoma / Foam cell / LDL / Atherosclerosis / Macrophage / Lipid peroxide / Antioxidants / Hyaluronic acid
Research Abstract

Xanthoma develops by the infiltration of lipid-laden foam cells similarly to atherosclerosis. To clarify the precise processes of foam cell infiltrating lesions, xanthoma is more competent than atherosclerosis in the visual inspection and taking the lesional specimens. The following results were obtained by using rabbit experimental xanthoma tissues. 1) From the experimental xanthoma tissues, more negatively charged LDL than native LDL was obtained and it was revealed to transform macrophages to foam cells. These cationized lipoproteins may contribute to the recruitment of foam cells in xanthoma tissues. 2) Human LDL was made more negatively charged and contained a larger amount of lipid peroxides than native LDL by incubation with experimental xanthoma tissues that were made by intradermal injections of carrageenan on diet -induced hypercholesterolemic rabbits. Mouse peritoneal macrophages incorporated more oxidized LDL than native LDL and the macrophages transformed to foam cells. Th … More e oxidization was inhibited by several antioxidants, for instance superoxide dismutase, butylated hydroxytoluene, alpha-tocopherol and catalase. No different inhibitory effects were observed among these antioxidants. These findings indicate that extravasated LDL is oxidatively modified and contributes to foam cell formation in xanthoma tissues. However, the precise mechanisms of the oxidization were not revealed. 3) Intradermal injections of hyaluronic acid induced infiltration of foam cells on hypercholesterolemic rabbits . Hyaluronic acid formed soluble complexes in vitro with lipoproteins of human whole serum and also with isolated LDL and VLDL of hypercholesterolemic rabbits. Extravasated LDL and VLDL might form soluble complexes with hyaluronic acid and receive oxidative modification in the dermis. It was suggested that the oxidized lipoproteins induced infiltration of macrophages and the macrophages transformed to foam cells by incorporating the oxidized lipoproteins. 4) The studies on the following subjects have to be continued: a)expression of monocyte adhesion molecules on the endothelial cells by the LDL modified by incubation with the experimental xanthoma tissues, 2)characterization of apolipoproteins and lipid moieties of lipoproteins that have been modified by the experimental xanthoma tissues. Less

  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Hajime.Kodama,Mitunori.Ikeda,Rouzou.Ookawa,Yasuo.Yamamoto: "Hyaluronic acid as a foam cell inducing factor in the rabbits of two distinct type of hypercholesterolemia." 18th World Congress of Dermatology,1992,6,17,New York.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kouzou Ookawa,Mitunori Ikeda,Hajime Kodama: "Foam cell inducing activity of low density lipoprotein rnodified by experimintal xanthoma tissues." 18th World Congress of Dermatology,1992,6,17,New York.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kouzou Ookawa,Mitunori Ikeda,Hajime Kodama: "Oxidative modification of low density lipoprotein in experimental xanthoma tissues." Journal of Dermatological Science(学会抄録). 4. 133 (1992)

    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi