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1992 Fiscal Year Final Research Report Summary

Cell proliferation and cell kinetic studies in skin cancers.

Research Project

Project/Area Number 02670484
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionSapporo Medical College

Principal Investigator

SAGA Kenji  Sapporo Medical College, Department of Dermatology, Assistant professor, 皮膚科学講座, 講師 (10153925)

Co-Investigator(Kenkyū-buntansha) EGUCHI Hiroaki  Sapporo Medical College, Department of Dermatology, Instructor, 皮膚科学講座, 助手 (90223643)
Project Period (FY) 1990 – 1992
KeywordsBasal cell carcinoma / Bowen's disease / Bromodeoxyuridine / DNA labeling index / Flow cytometry / AgNOR
Research Abstract

We studied cell kinetics in human skin cancer using the following three methods, (1) in vitro bromodeoxyuridine labeling, (2) DNA quantification by flow cytometry, and (3) silver staining of nucleolar organizer region (AgNOR).
The DNA synthesizing cells in benign and malignant keratotic diseases including psoriasis, seborrheic keratosis, Bowen's disease and basal cell carcinoma as well as normal skin were studied to understand the pathophysiology of these diseases. Cells in S-phase were labelled by incubating small pieces of excised skin tumors in the tissue culture medium containing 100 muM 5-bromo-2'-deoxyuridine (BrdUrd) under 3 atmospheric pressures of 95%O_2-5%CO_2 for one hour at 37゚C. Incorporated BrdUrd was demonstrated with an immunohistochemical method using anti-BrdUrd monoclonal antibody.
We analyzed the DNA labelling using the labelling index and labelling pattern. The labelling pattern was examined to determine if the polarity in labelling was preserved or lost. Although ma … More lignant diseases tended to show higher DNA labelling index, neither DNA labelling index nor the loss of polarity alone was associated with the malignancy. However, high DNA labelling index concomitant with the loss of polarity in labelling in the tumor seemed to be characteristic of the malignancy.
We studied the presence of DNA-aneuploidy using flow cytometry mainly in squamous cell carcinoma and malignant melanoma. Both diseases tended to show DNA-aneuploidy in advanced diseases. Intra-epidermal carcinomas including Bowen's disease did not show DNA-aneuploidy with flow cytometry, however it does not necessarily mean the absence of DNA-aneuploidy in these diseases. It could be possible that diploid pattern due to surrounding normal cells covered the DNA-aneuploidy due to small number of malignant cells.
We applied AgNOR, which stained acidic protein associated with rRNA transcription, to malignant melanomas and basal cell carcinomas. In melanoma and basal cell carcinoma, the number of AgNOR was significantly higher than melanocytic nevi and normal epidermis respectively. Less

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Morimoto Y: "In vitro DNA synthesis of Keratinocytes in normal human skin,psoviasis,seborrherc Keratosis,Bowen's disease and basal cell carcinoma." British Journal of Dermatology. 125. 9-13 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森元 洋介: "正常皮膚および上皮性皮膚疾患におけるS期細胞の同定" Skin Cancer. 7. 270-275 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morimoto Y, Saga K, Bando M, Takahashi M: "In vitro DNA synthesis of keratinocytes in normal human skin, psoriasis, seborrheic keratosis, Bowen's disease and basal cell carcinoma. Br J Dermatol" 125. 9-13 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morimoto Y, Tanaka M, Saga K, Takahashi M: "Identification of S-phase cells in normal epidermis and various epithelial skin diseases." Skin Cancer [in Japanese]. 7. 270-275 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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